天津医药

• 实验研究 •    

组织工程自体骨修复兔大块骨缺损

曹晓娜1,熊蔚莉2,李敬华2,胡丽玲3,梁东春4,孙蓓5,左爱军2   

  1. 1. 天津医科大学代谢病医院内分泌研究所
    2.
    3. 天津医大代谢病医院内分泌研究所
    4. 天津医科大学内分泌研究所
    5. 医科大学代谢病医院
  • 收稿日期:2009-12-01 修回日期:2010-01-18 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 曹晓娜

Auto-tissue engineering bone for rabbit segmental bone defects

  • Received:2009-12-01 Revised:2010-01-18 Published:2010-04-15 Online:2010-04-15

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摘要： 目的:研究组织工程自体骨的植入对大块骨缺损修复的影响。方法:培养兔的自体原代骨髓间充质干细胞，制备壳聚糖-明胶网络/羟基磷灰石多孔材料-重组人BMP2（CS-Gel/HA－rhBMP-2）复合材料。将骨髓间充质干细胞作为种子细胞与CS-Gel/HA－rhBMP-2进行复合。截去兔桡骨中段7 mm长度的骨组织，建立骨缺损动物实验模型。实验组（每只兔的右侧）植入CS-Gel/HA-rhBMP-2-种子细胞复合材料，对照组（每只兔的左侧）植入CS-Gel/HA材料，两组均不做内外固定，逐层缝合伤口。分别在术后第4周、8周和12周对缺损处进行X-线评分和组织学评分。结果:术后第4周实验组X-线和组织学评分与对照组相比无明显差别， 8周和12周实验组X-线和组织学评分均优于对照组,具有统计学意义（P<0.05）。结论:实验中所用移植修复材料具有优异的骨传导性、骨诱导性，可以缩短骨缺损的修复时间。

关键词: 骨髓间充质干细胞, 组织工程自体骨, 骨缺损, 修复

Abstract: Objective: Implantation of auto-tissue engineering bone to cure the rabbit segmental bone defects. Methods:Culture the rabbit mesenchymal cell， prepare the auto-tissue engineering bone with CS-Gel/HA－rhBMP-2. Bone mesenchymal stem cells as seed cells and carry out complex CS-Gel/HA-rhBMP-2.The establishment of animal models of bone defect is to amputate forelimb mid-7mm length of the rabbit bone tissue . In experimental group（right side），treat the rabbit segmental bone defect model with CS-Gel/HA－rhBMP-2－seed cell,in control group (left side),the implantation is CS-Gel/HA.Not do internal and external fixation,sew up the wound layer by layer.Evaluate the grade of X-ray and histology after treatment 4,8,12 weeks.Results:There are no significant differences of X-ray grade and histology grade between experiment group and control group at 4 weeks after treatment, but the X-ray grade and histology grade is better in experiment group than in control group at 8,12weeks after treatment，data are statistically significant (p<0.05).Conclusion: The auto-tissue engineering bone can cure the rabbit segmental bone defects.

Key words: Bone mesenchymal cell, Auto-tissue engineering bone, Bone defect, Cure