天津医药

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转化生长因子-β1对绒毛膜癌JEG-3细胞增殖及Smad3、Smad7表达的影响

许倩1,李玉红2,李晓茹3   

  1. 1. 承德医学院基础医学研究所
    2. 承德医学院基础部
    3.
  • 收稿日期:2009-11-23 修回日期:2010-04-19 出版日期:2010-09-15 发布日期:2010-09-15
  • 通讯作者: 李玉红

Effect of Transforming Growth Factor-β1 on the Proliferation and smad3、 7 protein expression in JEG-3 cells

  • Received:2009-11-23 Revised:2010-04-19 Published:2010-09-15 Online:2010-09-15

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摘要： ［摘要］目的：观察TGF-β1对绒毛膜JEG-3癌细胞增殖能力及其smad3、7蛋白表达的影响。方法：体外培养人绒毛膜癌JEG-3细胞，MTT法检测TGF-β1对JEG-3细胞增殖能力的影响，western blotting 检测smad3、7蛋白的表达。结果：TGF-β1能够以剂量和时间依赖性的方式促进JEG-3细胞的增殖， JEG-3细胞中的Smad3蛋白的含量逐渐升高，且表现出一定的剂量和时间效应， 而Smad7蛋白的含量无显著改变。结论：外源性TGF-β1能够促进JEG-3绒癌细胞的增殖，具有良好的浓度效应和时间效应； Smad7蛋白应答缺失， Smad3蛋白表达异常增高，提示绒毛膜癌JEG-3细胞逃逸TGF-β1介导的生长抑制作用可能是由于TGF-β1/Smads信号通路下游发生异常。

关键词: 转化生长因子β1, 绒毛膜癌细胞, 细胞增殖能力, smad3蛋白, smad7蛋白

Abstract: 【ABSTRACT】Objective：To inwestigate the effect of TGF-β1 on the Proliferation and smad3、7 protein expression in JEG-3 cells.Methods： the human choriocarcinoma cell line JEG-3 were cultured in vitro.After exposed to TGF-β1, the proliferation of JEG-3 cells was measured by MTT assay. The espression of smad3、7 protein was measured by Western blotting .Conclusion：The external TGF-β1 can stimulate the proliferation of JEG-3 choriocarcinoma cells, and showing excellent concentration and timing effect. Smad7 protein response missing, Smad3 protein expression in abnormally high levels, suggesting that choriocarcinoma JEG-3 cells to escape TGF-β1-mediated -mediated growth inhibition may be due to the downstream signaling pathway TGF-β1/Smads exception occurs.

Key words: Transforming Growth Factor-β1, choriocarcinoma cell, proliferation, smad3 protein, smad7 protein