CD4+CD25+调节性T细胞在不同肝脏肿瘤中的表达及意义

CD4+CD25+调节性T细胞在不同肝脏肿瘤中的表达及意义

王俊,颜秉菊

辽宁医学院附属第一医院

收稿日期:2011-04-11 修回日期:2011-11-08 出版日期:2012-02-15 发布日期:2012-02-15
通讯作者: 颜秉菊

The study about expression and significance of CD4 + CD25 + T cell in different liver tumor

Received:2011-04-11 Revised:2011-11-08 Published:2012-02-15 Online:2012-02-15

王俊,颜秉菊. CD4+CD25+调节性T细胞在不同肝脏肿瘤中的表达及意义[J]. .

摘要/Abstract

摘要： 研究CD4+CD25+调节性T细胞(Treg)在不同肝脏肿瘤中的分布特点，评价其在肿瘤的发生和发展中的作用。方法：采用双重酶标免疫组化染色的方法测定不同肝脏肿瘤中Treg细胞的表达，分析其在肝癌中的表达特点及其意义。结果：正常肝组织、肝局灶性结节状增生(FNH)组中均未发现Treg细胞的表达。肝脏不典型腺瘤样增生(AAH)组、原发性肝癌(HCC)组中有Treg细胞的表达,且HCC组较AAH组增多(P=0.000)。同时本研究还显示在肝癌的癌旁组织中已有Treg细胞浸润，在肝癌组织中Treg细胞数量较癌旁组织增多(P=0.002)。肝癌组织中Treg细胞数量与病人性别、年龄、术前AFP浓度无关，而与肿瘤大小、肿瘤包膜及术前HBV-DNA水平有显著关系(P<0.05)。结论：Treg细胞的表达与肿瘤的发生和发展有关，在肿瘤免疫中起负调节作用，除去或减少肝癌微环境中的Treg细胞有可能提高肿瘤的免疫治疗效果。

关键词: CD4+CD25+调节性T细胞, 肝局灶性结节状增生, 肝脏不典型腺瘤样增生, 原发性肝癌。

Abstract: Objective To study the distribution of CD4 + CD25 + regulatory T cells (Treg) in normal liver tissue,the liver focal nodular hyperplasia(FNH),atypical adenomatous hyperplasia of the liver(AAH) , hepatocellular carcinoma (HCC) and side of cancer. We will evaluate its the role in tumor occurrence and development. Methods The expression of treg cells were measured in 90 cases with double ELISA method of immunohistochemical staining. Analysis of its expression and significance in hepatocellular carcinoma.Rusult Normal liver tissue, FNH group were not found in the expression of Treg cells. AAH and HCC group have the expression of Treg cells. Treg cells of HCC group was signifieantly higher than in AAH group(P=0.000), The expression of Treg Cells was no significant difference between different age and sex and preoperative AFP value (P> 0.05); It is a relationship between treg cells number and the size of tumor, Treg cells of Enveloped group was signifieantly higher than in no capsule group,Comparison between normal and upper groups of Preoperative HBV-DNA was statistically significant difference.(P=0.007).Conclusion The increase of Treg cells favor occurrence and development of the tumor. It is plays a negative regulatory role in the immune of the tumor. Remove or reduce the Treg cells of the liver micro-environment may increase the effect of tumor immunotherapy.

Key words: CD4+CD25+regulatory T cells, The liver focal nodular hyperplasia, Atypical adenomatous hyperplasia of the liver, Hepatocellular carcinoma