天津医药

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应用DiversiLab系统对肺炎克雷伯菌进行同源性分析

李静   

  1. 天津医科大学总医院医学检验科
  • 收稿日期:2013-01-18 修回日期:2013-07-16 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 李静

The Application of DiversiLab System inKlebsiella pneumoniaeGenotyping

LI Jing   

  1. Department of Clinical Laboratory, General Hospital of Tianjin Medical University
  • Received:2013-01-18 Revised:2013-07-16 Published:2013-11-15 Online:2013-11-15
  • Contact: LI Jing

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摘要：

 【摘要】 目的  应用DiversiLab系统对肺炎克雷伯菌进行同源性分析，为医院感染控制工作提供依据。 方法  对2010年天津一家甲级医院外科同一病房临床分离的8株肺炎克雷伯菌，应用以细菌基因组重复序列PCR技术（rep-PCR）为原理的DiversiLab系统分型技术进行分型，并与脉冲场凝胶电泳（PFGE）分型结果进行比较。  结果  药敏试验结果显示，除K8-02号菌株外，其余7株肺炎克雷伯菌对11种抗菌药物具有相同的药敏谱，且为多重耐药菌；PFGE分析显示此7株菌为同一型；DiversiLab系统同时也显示该7株菌为同一组，K8-02菌为单独一组。  结论  DiversiLab系统的操作简单、快速、可重复性好、结果准确，是一种标准化和自动化系统，并且DiversiLab系统与PFGE方法可获得一致结论

关键词: 克雷伯菌, 肺炎, 电泳, 凝胶, 脉冲场, 细菌分型技术, DiversiLab系统

Abstract:

[Abstract]   Objective  To analyzeKlebsiella pneumoniaeby DiversiLab system, providing scientific evidence for the
control of nosocomial infection.  Methods  Eight strains of non-duplicated clinicalKlebsiella pneumoniaeisolated from the surgical ward in a hospital in2010were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis (PFGE).   Results   Antimicrobial susceptibility test showed that7clinical strains were the same sensitivity to 11antimicrobial agents, except the strain K8-02. And these 7clinical strains were all multi-drug resistant. The result of PFGE showed that7strains were the same pattern. The result of DiversiLab also showed that7strains were the same pattern, and the strain K8-02was another pattern.   Conclusion   DiversiLab system is simple, quick, good repeatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.

Key words: Klebsiellapneumoniae, electrophoresis, gel, pulsed-field, bacterial typing techniques, DiversiLab system