天津医药

• 实验研究 •    

丝素蛋白的相关生物学性能研究

徐广涛1,周新妹2,于海宁3,徐国文2,吴晓薇2,郑 漾2,俞杭斌2,林天送2,陈士票2   

  1. 1. 嘉兴学院医学院
    2.
    3. 浙江大学药学院药学系
  • 收稿日期:2009-12-09 修回日期:2010-01-26 出版日期:2010-09-15 发布日期:2010-09-15
  • 通讯作者: 于海宁

Investigation on the Biological Performance of Silk Protein

  • Received:2009-12-09 Revised:2010-01-26 Published:2010-09-15 Online:2010-09-15

PDF (PC)

2043

摘要： 摘要 目的：检测丝素蛋白的抑菌活性、抗紫外线能力、吸湿性、保湿性及皮肤致敏性，为开发丝素蛋白提供科学的基础数据。方法：通过人工的方法将丝素蛋白制成5种不同大小颗粒，配制相应的溶液。利用平板菌落计数法、吸光度法、饱和碳酸钙溶液吸湿率法、致敏接触及激发接触法分别检测丝素蛋白的各项生物学性能。结果：5种丝素蛋白溶液均有一定抑菌性，对大肠杆菌的生长抑制效果优于对金黄色葡萄球菌。丝素肽、3 μm丝素粉、大分子量丝素肽的抗紫外线时间在1h以上。大分子量丝素肽和丝素肽的吸湿能力较好，48 h吸湿率分别为44.78％和35.30％。3 μm丝素粉和丝素肽的保湿能力最强，48 h保湿率分别为82.29％和78.91％。丝素蛋白液对豚鼠皮肤均无致敏性。结论：丝素蛋白可用于研发新类型的化妆产品。

关键词: 丝素蛋白, 抑菌活性, 抗紫外线能力, 吸湿性, 保湿性, 致敏性

Abstract: Abstract Objective: To detection bacteriostatic activity, uvioresistant ability, hygroscopicity, moisture and dermal sensitization of silk protein and to furnish information for product development of silk protein. Methods: Silk protein was divided into 5 groups of varying size according to artificial, and prepared to solution. To detection related biologic activity by using plate bacterial colony count, absorption, moisture absorption rate of saturated calcium carbonate solution, allergen exposure and stimulate contact. Results: The silk protein had certain antibacterial ability, and the effect of growth inhibitory was excellent on E. coli than Staphylococcus aureus. The uvioresistant time of silk peptide, 3 μm silk protein powder and high molecular weight silk peptide lasted more than 1h. High molecular weight silk peptide and silk peptide had better hygroscopic, and hygroscopicity rate after 48 h were 44.78% and 35.30%. 3 μm silk protein powder and silk peptide had better moisture retention and moisture rate after 48 h were 82.29% and 78.91％. Silk protein was non-allergenicity on guinea-pig skin. Conclusion: The new cosmetics composed of silk protein may be researched and developed.

Key words: silk protein, bacteriostatic activity, uvioresistant ability, hygroscopicity, moisture retention, sensitization