雌二醇对HepG2细胞载脂蛋白AⅠmRNA表达的影响

雌二醇对HepG2细胞载脂蛋白AⅠmRNA表达的影响

崔丽¹,毛用敏²,赵莉莉²,崔让庄²

1. 天津医科大学研究生院博士在读；天津市南开医院
2. 天津市胸科医院

收稿日期:2009-12-16 修回日期:2010-02-21 出版日期:2010-10-15 发布日期:2010-10-15
通讯作者: 毛用敏

The Effects of Estradiol on Apolipoprotein AⅠ mRNA Transcription in HepG2 Cells

Received:2009-12-16 Revised:2010-02-21 Published:2010-10-15 Online:2010-10-15

崔丽1 ,毛用敏2 ,赵莉莉2 ,崔让庄2 . 雌二醇对HepG2细胞载脂蛋白AⅠmRNA表达的影响[J]. .

摘要/Abstract

摘要： 目的：探讨雌激素对人肝癌细胞株（HepG2细胞）Apo AⅠmRNA表达的影响。方法：分别将0.01、0.1、1.0、10.0、20.0μmol/L雌二醇与体外培养的HepG2细胞共同孵育24h；选用10μmol/L的雌二醇与HepG2细胞共同孵育0 h、1h、6h、24h、48h；收集细胞提取总RNA，采用逆转录聚合酶链反应(RT-PCR)测定Apo AⅠmRNA表达水平。结果：随雌二醇浓度的增加，HepG2细胞内Apo AⅠmRNA 表达呈现先升高后降低的趋势，以10μmol/L时表达最高，与空白组及0.01、0.1、1μmol/L组相比有明显差异（P<0.05），20μmol/L时Apo AⅠmRNA表达降低；时间刺激试验显示随刺激时间的延长，HepG2细胞中Apo AⅠmRNA 表达呈现先升高后降低的趋势，刺激6h 后表达水平增加，与0 h、1h 表达水平相比差异有统计学意义（P<0.05）；刺激24h后表达水平最高（P<0.05）。结论：雌二醇可在转录水平调节Apo AⅠ基因表达，并呈现部分的剂量和时间依赖性。

关键词: 雌激素、载脂蛋白AⅠ、逆转录聚合酶链反应

Abstract: Objective To observe the effect of estrogen on modulating apolipoprotein AⅠ gene transcription in HepG2 cells. Methods Exposure HepG2 cells to different concentration of estradiol (0.01,0.1,1.0,10.0,20.0 μmol/L) for 24 hours, respectively; HepG2 cells were incubated with estradiol at 10μmol/L concentration for 1,6,24,48 hour. RNA was extracted from HepG2 cells collected at above time points and the expression levels of apoAⅠmRNA was analysized by RT-PCR. Results The level of apoAⅠmRNA expressed in HepG2 cells stimulated by different concentrate of estradiol was in a dose-dependent increased. Compared with 0.01, 0.1, 1μmol/L of estradiol, apoAⅠ mRNA was significantly higher in 10μmol/L of estradiol; A time-course experiment at the 10μmol/L of estradiol indicated that the expression of apoAⅠmRNA was significantly increased at 6 hour(P<0.05)and the highest at 24 hour(P<0.05). Conclusions Estrogen may play an important role in the transcription of apoAⅠgene, the effect for mRNA expression show a dose-dependent and time-dependent partly.

Key words: estrogen、apolipoprotein AⅠ、RT-PCR