天津医药

• 实验研究 •    

塞来昔布对急性肺损伤大鼠肺组织NF-κB和iNOS的影响

邢志伟1,张建武2,梁玉庚3,吕翠环1,杜月菊1,刘锐4,孙红梅1   

  1. 1. 河北省胸科医院检验科
    2. 河北省胸科医院输血科
    3. 石家庄市长城中西医结合医院
    4. 河北省胸科医院结核三病区
  • 收稿日期:2013-01-09 修回日期:2013-08-05 出版日期:2013-10-15 发布日期:2013-10-15
  • 通讯作者: 邢志伟

Effects of Celecoxib on NF-κB and iNOS in Rat Model of Acute Lung Injury

XING Zhi wei1,ZHANG Jian wu2,LIANG Yu geng3,Lü Cui huan1,DU Yue ju1,LIU Rui 4,SUN Hong mei1   

  1. 1. Department of Clinical Laboratory of Hebei Chest Hospital
    2. Department of BloodTransfusion, Hebei Chest Hospital
    3. Great Wall Chinese and Western Medicine Hospital, Shijiazhuang
    4. The Third Tuberculosis District of Hebei Chest Hospital
  • Received:2013-01-09 Revised:2013-08-05 Published:2013-10-15 Online:2013-10-15
  • Contact: XING Zhi wei

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摘要：

 【摘要】  目的  探讨脂多糖（LPS）所致大鼠急性肺损伤时环氧化酶-2（COX-2）抑制剂塞来昔布对肺组织的核因子-κB（NF-κB）p65和诱导型一氧化氮合酶（iNOS）表达的影响。  方法  将60只大鼠随机分为对照组、LPS组、治疗组和塞来昔布组，每组15只。LPS组尾静脉注射LPS（5mg/kg）复制急性肺损伤模型；治疗组注射LPS复制急性肺损伤模型后30min用塞来昔布灌胃（20mg/kg）；塞来昔布组不造模，于相同时间点用相同剂量塞来昔布灌胃；对照组不造模、不给药，给等量的生理盐水；各组均于3h后放血处死动物。采用蛋白质印迹（Western blot）和逆转录-聚合酶链反应（RT-PCR）方法分别检测肺组织COX-2、NF-κB p65、iNOS蛋白及NF-κB p65、iNOS mRNA的表达。  结果  LPS组与对照组相比COX-2、NF-κB p65和iNOS蛋白及NF-κB p65、iNOS mRNA表达均显著升高（P<0.01）；治疗组NF-κB p65、iNOS mRNA和蛋白表达较LPS组明显降低（P<0.01）。  结论  选择性COX-2抑制剂塞来昔布对急性肺
损伤大鼠有保护作用。

关键词: 急性肺损伤, NF-κB, 一氧化氮合酶, 环氧化酶2抑制剂, 环氧化酶2

Abstract:

[Abstract]   Objective   To investigate the effects of celecoxib on the expressions of nuclear factor-κB (NF-κB) p65
and inducible nitric oxide synthase (iNOS) in rat model of acute lung injury induced by lipopolysaccharide (LPS).  Methods   Sixty rats were randomly divided into control group, LPS group, treatment group and celecoxib group (15rats for each group).To copy the rat model of acute lung injury, LPS（5mg/kg）was injected into the tail vein in LPS group. Celecoxib (20mg/kg）was administered by gavage after30-minute modeling in treatment group. Celecoxib (20mg/kg）was also administered by gavage in celecoxib group. The same volume of normal saline was treated in control group. Rats were sacrificed after3h in four groups. The expressions of cyclooxygenase-2(COX-2), NF-κB p65and iNOS protein were detected by Western blot analysis. The expressions of NF-κB p65and iNOS mRNA were evaluated by reverse transcription polymerase chain reaction (RT-PCR) assay.  Results  Compared with control group, expression levels of COX-2, NF-κB p65, iNOS protein,NF-κB p65 and iNOS mRNA were significantly higher in LPS group (P<0.01). The NF-κB p65, iNOS mRNA and protein expressions were significantly decreased in treatment group than those of LPS group (P<0.01).   Conclusion   The selective COX-2inhibitor celecoxib has a protective effect on acute lung injury in rats.

Key words: Acutelunginjury, NF-kB, nitric oxide synthase, cyclooxygenase2inhibitors, cyclooxygenase 2