天津医药

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mfn2和nm23在不同膀胱组织中的表达

白云   

  1. 河北联合大学生命科学学院
  • 收稿日期:2013-10-31 修回日期:2014-06-03 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 白云

Expression of Mitofusin-2 (mfn2) and nm23 in Different Bladder Tissues

BAI yun   

  • Received:2013-10-31 Revised:2014-06-03 Published:2014-10-15 Online:2014-10-15
  • Contact: BAI yun

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摘要：

【摘要】目的 探讨线粒体融合蛋白-2(mfn2)和肿瘤转移抑制基因（nm23）在膀胱癌组织中的表达及与临床病理特征间的关系。方法 选取65 例膀胱癌标本，其中男50 例，女15 例。TNM 分期：Ⅰ期47 例、Ⅱ期10 例、Ⅲ期5 例、Ⅳ3 例，另择正常膀胱组织、良性膀胱肿瘤标本各15 例作为对照。应用免疫组化SP 法检测mfn2 和nm23 的表达情况，并分析其在膀胱癌组织中的表达与临床病理特征之间的关系。结果 膀胱癌组织中mfn2 的阳性表达率均高于正常和良性膀胱组织，nm23 的阳性表达率均低于正常和良性膀胱组织（均P＜0.05）。膀胱癌组织中mfn2 的表达在不同分化程度、TNM 分期间的差异有统计学意义，在不同年龄、性别、淋巴结转移情况和临床分期间的差异无统计学意义。nm23 在不同TNM 分期、淋巴结转移情况和临床分期间的差异有统计学意义，在不同年龄、性别和分化程度间的差异无统计学意义。结论mfn2 在膀胱癌中高表达，mfn2 与膀胱癌的发生、发展密切相关；nm23 在膀胱癌中低表达，可作为预测膀胱癌转移及其预后的参考指标。

关键词: 膀胱肿瘤, mfn2, nm23, 免疫组织化学

Abstract:

[Abstract] Objective  To research the expression of mitofusin-2 (mfn2) and tumor metastasis suppressor genes （nm23）in bladder cancer cells and its correlation with clinical pathological feature． Methods Immunohistochemistry was used to measure the expression of mfn2 and nm23．Sixty-five cases of bladder cancer were sampled，which include fifty cases of male and fifteen cases of female. TNM stage：Forty-seven cases were in stage I; Ten cases were in stage II; Five cases were in stage Ⅲ; Three cases were in stageⅣ. Other fifteen cases were sampled from normal bladder or benign tumor of bladder as control . All cases were collected from department of pathology，affiliated Hospital of hebei union university. Results  The positive expression rate of mfn2 in bladder cancer tissues was significantly higher than those in normal bladder tissues and benign tumor of bladder（χ2 =32.528，P < 0.05）；The positive expression rate of nm23 in bladder cancer tissues was significantly lower than those in normal bladder tissues and benign tumor of bladder (χ2 =19.719，P<0.05)；the high expression level of mfn2 in bladder cancer was associated with tumor differentiation and TNM stage（P＜0.05），but not correlated with age，sex，lymph node metastasis and clinical grade. The low level of nm23 was associated with TNM stage，clinical grade and LN metastasis. Conclusion  The positive expression rate of mfn2 was increased in bladder cancer. It indicated that there was a close relationship between mfn2 and the occurrence and development of bladder cancer；The expression rate of nm23 was decreased in bladder cancer，it may be a predictor for metastasis and prognosis of bladder cancer.

Key words: urinary bladder neoplasms, mfn2, nm23, immunohistochemistry