天津医药

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嗜麦芽窄食单胞菌生物被膜形成的体外抗菌活性研究

梁歌宏1,祁伟2,宋诗铎3,孙二琳4,王哲5,6   

  1. 1. 天津医科大学第二医院 感染性疾病研究所
    2. 天津医科大学第二医院感染性疾病研究所
    3. 天津医科大学第二医院 天津市感染性疾病研究所
    4. 天津医科大学第二医院 天津市泌尿外科研究所
    5. 天津医科大学第二医院 感染性疾病研究所
    6.
  • 收稿日期:2010-11-01 修回日期:2011-03-14 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 梁歌宏

In vitro Susceptibility Assay of 51 Stenotrophomonas Maltophilia and with biofilms to antibiotics

  • Received:2010-11-01 Revised:2011-03-14 Published:2011-08-15 Online:2011-08-15

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摘要： 要 目的：研究天津地区嗜麦芽窄食单胞菌(SMA)感染与耐药现状，观察临床常用抗生素对其形成生物被膜后的作用效果。方法：分析临床分离的非重复51株SMA对12种抗生素（左氧氟沙星、氧氟沙星、环丙沙星、哌拉西林、头孢曲松、头孢他啶、头孢哌酮、头孢哌酮/舒巴坦、头孢吡肟、头孢噻肟、阿米卡星、庆大霉素）的耐药情况，通过建立SMA生物被膜的体外模型，测定对产生物被膜的抑制浓度（BIC），与相应抗生素的最低抑菌浓度（MIC）进行比较。并行扫描电镜观察抗生素作用后细菌生物被膜的形态学变化。结果：51株SMA对12种常用抗生素的耐药率分别是：13.73％；25.49％；37.25％；45.10％；88.25％；60.78％；49.02％；43.14％；45.10％；94.12％；90.20％；82.35％；其中42株SMA利用微量接种针成功建立成熟生物被膜的体外模型；经药敏实验和扫描电镜证实，形成生物被膜后，每种抗生素的耐药菌株数和耐药程度均大幅度增加。结论：本地区分离的SMA具有耐药谱广、耐药率高特点；左氧氟沙星和头孢哌酮/舒巴坦对SMA有较强的抗菌活性。与浮游态细菌相比，形成生物被膜后SMA对抗生素的耐药程度增加。

关键词: 嗜麦芽窄食单胞菌, 生物被膜, 最低抑菌浓度, 生物被膜抑制浓度, 抗生素

Abstract: Abstract Objective: To investigate the current situations and resistance of Stenotrophomonas Maltophiliaclinical strains collected from Tianjin Area, we investigated the in vitro effects of antibiotics on the SMA biofilms. Methods: The distribution and drug resistant situations of clinical strains of SML isolated were analyzed. Subseqently, The model of SMA biofilms in vitro was developed, challenged by antibiotics and calculated biofilm inhibitory concentrations, and to observe the change of biofilms by scanning electron microscopy. Rusults: The resistance rates of SMA were as following: 13.73％；25.49％；37.25％；45.10％;88.25％；60.78％；49.02％；43.14％；45.10％；94.12％；90.20％；82.35％; Of 42 strains which could be formed bacterial biofilms; The drug experiments and scanning electron microscopeand biofilms formation that, after each antibiotic resistance and resistance strains were increased substantially. Conclusion: SMA may be one of the most important pathogenic bacteria in Tianjin Area, with features of high drug resistance rate and multidrug resistance. The susceptibility testings demonstrate, levofloxacin and cefoperazone/sulbactam have high level antimicrobial activity. Comparing to planktonic bacterial growth, SMA growing as biofilms proves to be highly resistant to most antimicrobials.

Key words: Stenotrophomonas Maltophiliaclinical, biofilm, minimal inhibitory concentration, biofilm inhibitory concentration, antibiotics