天津医药

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塞来昔布联合PDTC对人胃癌细胞株SGC-7901生长的抑制作用[ 作者单位：300070 天津医科大学研究生院（夏秀丽），天津医科大学第二医院（张志广，李熳，闻淑军）

夏秀丽1,张志广2,李熳3,闻淑军4   

  1. 1. 天津医科大学
    2. 天津医科大学第二医院
    3. 天津医大二院消化科
    4. 医大二院消化科
  • 收稿日期:2010-11-25 修回日期:2011-04-25 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 张志广

The inhibition of celecoxib combined with PDTC on SGC-7901 gastric cancer cells

  • Received:2010-11-25 Revised:2011-04-25 Published:2011-10-15 Online:2011-10-15

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摘要： 摘要 目的：研究塞来昔布及吡咯烷二硫氨基甲酸（PDTC）对人胃癌细胞株SGC-7901的生长抑制作用及其相关机制。方法:分别应用50、100 μmol/L的塞来昔布、PDTC及2药联合（25/25、50/50 μmol/L）处理SGC-7901胃癌细胞，四甲基偶氮唑蓝(MTT)法检测细胞生长抑制率。TUNEL法检测细胞的原位凋亡。RT-PCR检测环氧合酶-2（COX-2）、核因子（NF）-kB、caspase-3 mRNA的表达。结果：塞来昔布、PDTC及2药联合作用于细胞72 h后细胞的生长均受到抑制（P<0.05），25/25 μmol/L的2药联合组与50μmol/L塞来昔布组相比，细胞的生长抑制率无明显差异（P>0.05）。50/50 μmol/L的2药联合组细胞生长抑制率高于100μmol/L的单独用药组（P<0.05）。100μmol/L的单独用药组和50/50 μmol/L的2药联合组在作用于细胞24 h后，细胞凋亡指数均高于阴性对照组，且2药联合组凋亡指数高于单独用药组(P<0.05)。100μmol/L的塞来昔布及PDTC单独作用于细胞12 h后均可见COX-2、NF-kB表达降低(P<0.05)，caspase-3表达升高(P<0.05)，50/50 μmol/L的2药联合组与100μmol/L的单独用药组相比，表达变化更明显(P<0.05)。结论:塞来昔布与PDTC联合应用具有显著抗胃癌细胞增殖，促进其凋亡的作用，其机制可能与抑制COX-2、NF-kB的表达，促进caspase-3表达有关。

关键词: 胃肿瘤, NF-κB, 细胞凋亡, 环氧化酶2, 塞来昔布, 吡咯烷二硫氨基甲酸, SGC-7901

Abstract: Abstract Objective:To investigate the effect of inhibition and its mechanism about the selective COX-2 inhibitor celecoxib， and NF-kB inhibitor pyrrolidine dithiocarbamate (PDTC) on human gastric cancer cell line SGC-7901.Methods:SGC-7901 cells were respectively treated in vitro with celecoxib (50、100 μmol/L)and PDTC(50、100 μmol/L) or combination（25/25、50/50 μmol/L）. The methyl thiazolyl tetrazolium (MTT) assays were used to measure the growth inhibition rate of cells. TUNEL assays were used to measure the in situ apoptosis of gastric cancer cells .RT-PCR assays the expression levels of COX-2、NF-kB、caspase-3. Results:The growth of gastric cancer cells were inhibited after treated 72 hours with celecoxib， PDTC or the combination group (P<0.05)，but the combination groups(25/25 μmol/L)didn't do more than the celecoxib (50 μmol/L)groups. The combination groups(50/50 μmol/L) are more effective than single drugs with 100μmol/L(P <0.05). After treated 24 hours by the groups of celecoxib（100μmol/L） ，PDTC （100 μmol/L）or combination groups（50/50 μmol/L），the apoptosis index was significantly different compared with negative control，and the combination groups had higher effect (P <0.05).the expression of COX-2、NF-kB were decreased (P <0.05)and caspase-3 was increased (P <0.05) when the cells had been exposed to celecoxib （100 μmol/L）or PDTC （100μmol/L）by 12 hours，and compared with them，the combination groups (50/50 μmol/L) had obvious changes(P <0.05).Conclusion:Both the celecoxib and PDTC can inhibite the proliferation and promote the apoptosis of gastric cancer cells.the mechanism may associated with inhibiting the expression of NF-kB、COX-2，then promote the expression of caspase-3.

Key words: stomach neoplasms, nf-kappa b, apoptosis, cyclooxygenase 2, Celecoxib, PDTC, gastric cancer cellsSGC-7901