天津医药

• 论著 •    下一篇

宫颈病变组织中PKR与NF-κB p65的表达与磷酸化观察

罗远材,郭路   

  1. 天津市第一中心医院妇产科
  • 收稿日期:2013-03-28 修回日期:2013-07-24 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 罗远材

Expression and Phosphorylation of PKR and NF-κB p65in Cervical Lesions

LUO Yuan cai,GUO Lu   

  1. Department of Gynecology and Obstetrics, Tianjin First Central Hospital
  • Received:2013-03-28 Revised:2013-07-24 Published:2013-11-15 Online:2013-11-15
  • Contact: LUO Yuan cai

PDF (PC)

2091

摘要：

【摘要】 目的  探讨宫颈病变组织内蛋白激酶R（PKR）、核因子（NF）-κB p65的表达及磷酸化的意义，高危型人乳头状瘤病毒（hsHPV）对两者表达及磷酸化的影响，以及三者的关系。  方法  以67例宫颈癌、149例宫颈上皮内瘤样病变（CINⅠ~Ⅲ）、15例正常人宫颈组织为观察对象。检测各组hsHPV阳性表达情况，采用免疫组化SP法检测组织内PKR、磷酸化型PKR（p-PKR）、NF-κB p65和磷酸化型NF-κB p65（p-NF-κB p65）在上述分组中的表达。  结果  231例中hsHPV阳性136例，阴性95例。胞浆中PKR、p-PKR在hsHPV阳性组的表达低于hsHPV阴性组（27.2%和11.0%vs41.1%和21.1%，χ2分别为4.858、4.371，均P＜0.05），在胞浆和胞核中NF-κB p65、p-NF-κB p65在hsHPV阳性组的表达高于hsHPV阴性组（46.3%、25.7%、22.8%和12.5%vs32.6%、14.7%、11.6%和4.2%，χ2分别为4.345、
4.048、4.729、4.650，均P＜0.05）；在胞浆和胞核中NF-κB p65（+）组PKR的阳性表达率低于NF-κB p65（-）组（25.5%vs38.0%和20.4%vs36.3%，χ2分别为3.898、4.396，P＜0.05），p-NF-κB p65（+）组在胞浆中PKR的阳性表达率低于pNF-κB p65（-）组（19.0%vs36.0%，χ2=4.462，P＜0.05）。  结论  hsHPV可能抑制PKR的表达及磷酸化而促进NF-κBp65的表达及磷酸化，NF-κB p65的表达及磷酸化对PKR的表达可能有抑制作用；三者间的调节作用可能与宫颈癌的发生发展有关。

关键词: 宫颈肿瘤, 宫颈上皮内瘤样病变, 蛋白激酶类, eIF-2激酶, 磷酰化, NF-κ

Abstract:

[Abstract]  Objective   To identify the significance of expression and phosphorylation of protein kinase R(PKR) and
nuclear factor NF-κB p65in cervical lesions, and the effect of high-risk human papilloma virus（hsHPV) on expression and phosphorylation of R(PKR) and NF-κB p65.  Methods  A total of67patients with cervical cancer,149patients with cervical intraepithelial neoplasia (CINⅠ-Ⅲ) and15normal control were included in this study. The expression levels of PKR,phosphorylated PKR (p-PKR), NF-κB p65and phosphorylated NF-κB p65(p-NF-κB p65) were detected by immunohistochemical SP method in three groups.  Results  The positive expression rates of PKR and p-PKR in cytoplasm were significantly lower in hsHPV positive group than those in hsHPV negative group (27.2% and11.0%vs41.1% and21.1%，χ2=4.858and4.371，P＜0.05). The positive expression rates of NF-κB p65and p-NF-κB p65in cytoplasm and nucleus were significantly higher in hsHPV positive group than those in hsHPV negative group (46.3% ,25.7% ,22.8% and12.5%vs32.6%,14.7%,11.6% and4.2%,χ2=4.345,4.048,4.729and 4.650 respectively，P＜0.05). The positive expression rates of PKR in kytoplasm and karyon were significantly lower in NF-κB p65(+) group than those in NF-κB p65(-) group (25.5%vs38.0% and20.4%vs36.3%，χ2=3.898and4.396respectively, P＜0.05). The positive expression rate of PKR in kyto?plasm was significantly lower in p-NF-κB p65(+) group than those in p-NF-κB p65(-) group (19.0%vs36.0%,χ2=4.462，P＜0.05).  Conclusion  hsHPV may inhibit the expression and phosphorylation of PKR but promote the expression andphosphorylation of NF-κB p65. The expression and phosphorylation of NF-κB p65may inhibit the expression of PKR. Regulating effects of three may be associated with the generation and progression of cervical cancer.

Key words: uterine cervical neoplasms, cervical intraepithelial neoplasia, protein kinases, eIF-2kinase, phosphory-lation, NF-kappa B