天津医药

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50种分枝杆菌标准株分枝菌酸指纹谱特征性研究

王巨存1,赵凤仪1,王学谦1,陈保文2,王国治2,陆芸1   

  1. 1. 天津市天津医院
    2. 中国药品生物制品检定所
  • 收稿日期:2010-02-25 修回日期:2010-05-11 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 王巨存

The characteristic study on the mycolic acids fingerprintings of 50 standard strains of mycobacteria

  • Received:2010-02-25 Revised:2010-05-11 Published:2010-10-15 Online:2010-10-15
  • Contact: WANG Ju-Cun

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摘要： 摘要 目的:用本实验室所建立的高效液相色谱（HPLC）分析分枝菌酸（MA）的方法，构建50种分枝杆菌标准菌株的MA指纹图谱库，并对该库指纹图谱进行比较研究。 方法: 经过分枝杆菌的接种、培养、皂化、酸化，提取到MA。MA衍生化后，采用HPLC进行分析，得到相应的指纹图谱。依据谱图的相对保留时间、相对峰高详细分析与比对其特征性。结果: 通过对上述分枝杆菌MA的色谱分析，构建了50种分枝杆菌标准菌株的MA指纹谱库。上述图谱可分为三组。即：单族峰型（single-cluster peak patterns）、两族峰型（double-cluster peak patterns ）、三族峰型（triple-cluster peak patterns）。每组分别包括10、23和17种。依据谱图的特征性，对50种分枝杆菌进行了分型。在92个临床分离株中，有89株的鉴定结果与已知结果一致，一致率为96.74%。结论: 所构建的指纹图谱全部具有特征性，所建立的方法可对上述分枝杆菌进行快速和可靠的种间鉴定。

关键词: 分枝杆菌, 分枝菌酸, 色谱法 高效液相, 指纹图谱, 特征性

Abstract: Tianjin Hospital,Tianjin 300211,China Abstract Objective: The fingerprintings library of 50 standard strains of mycobacteria was constructed by mycolic acid analysis using high performance liquid chromatography(HPLC) .The characteristic study on the fingerprintings was carried out. Methods: Mycolic acids were extracted ,derivatized and analyzed by the HPLC method after mycobacterium species were cultured ,saponified and acided. The mycolic acids fingerprintings library was set up. On the basis the HPLC patterns of relative retention time and relative peak height, the characteristic of the fingerprintings was analyzed and compared. Results: The mycolic acids fingerprintings library of 50 standard stains of mycobacteria was constructed by mycolic acid analysis using HPLC .These patterns may be divided into three groups,such as single-,double- and triple-clusters peak patterns,each groups comprising 10,23,and 17 species, respectively.50 strains were successfully typing by its characteristics. Of 92 isolates tested,89 were given species names in concordance with the known names. The concordance rate was 96.74%.Conclusions: All of patterns have characteristics. The established method can be used to identify above mycobacterium species rapidly and reliably.

Key words: Mycobacteria, Mycolic acids, Chromatography high performance liquid, Fingerprintings, characteristic