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地西他滨联合全反式维甲酸对HL-60细胞增殖及分化的影响

吕海容   

  1. 天津市第一中心医院血液科
  • 收稿日期:2012-03-12 修回日期:2012-09-17 出版日期:2013-02-15 发布日期:2013-02-15
  • 通讯作者: 吕海容

Effect of Decitabine Combined with ATRA on Proliferation and Differentiation of HL-60 Cells

LU Hai rong   

  1. Department of Hematology, Tianjin First Central Hospital, Tianjin 300192, China
  • Received:2012-03-12 Revised:2012-09-17 Published:2013-02-15 Online:2013-02-15
  • Contact: LU Hai rong

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摘要：

【摘要】目的 探讨地西他滨（DCA）和全反式维甲酸（ATRA）对急性早幼粒细胞白血病（APL）细胞株HL-60增殖及分化的影响。方法 HL-60细胞经不同浓度DCA、ATRA、两药单用或联合处理后，用四甲基偶氮唑盐（MTT）比色法观察不同浓度DCA和ATRA对HL-60细胞增殖活性的影响，用硝基四氮唑蓝（NBT）还原试验和流式细胞术观察不同浓度DCA和ATRA对HL-60细胞分化的影响。结果 DCA和ATRA均能够抑制HL-60细胞的增殖，呈时间、剂量依赖性（P < 0.01），并且3.2 mmol·L-1 DCA联合1×10-6 mol·L-1 ATRA组与单用两药比较，其增殖抑制的协同作用明显增强（P < 0.05）。DCA和ATRA均能够促进HL-60细胞的分化，3.2 mmol·L-1 DCA和1×10-6 mol·L-1 ATRA的促分化能力最强，并且两药联合应用具有协同促分化作用。结论 DCA和ATRA均可以抑制HL-60细胞的增殖以及促进 HL-60细胞分化，两者联合应用有协同作用。

关键词: 地西他滨, 全反式维甲酸, HL-60细胞株, 急性早幼粒细胞白血病

Abstract:

[Abstract] Objective  To investigate the effect of demethylation agent decitabine (DCA) and all-trans retinoic acid (ATRA) on proliferation and differentiation of acute promyelocytic leukemia (APL) cell line HL-60. Methods  HL-60 cells were co-cultured with different concentrations of DCA and/or ATRA. The proliferation activity was detected by MTT assay in HL-60 cells. The differentiation ability of HL-60 cells was observed by nitro-blue tetrazolium (NBT) reduction test and flow cytometry. Results  The proliferation of HL-60 cells was inhibited by DCA and ATRA in a dose and time dependent manner (P < 0.01). The concentration of 3.2 mmol·L-1 DCA combined with 1×10-6 mol·L-1 ATRA showed a more obvious inhibition on proliferation of HL-60 cells compared with that of DAC or ATRA alone (P < 0.05). Both DCA and ATRA could promote differentiation of HL-60 cells. The strongest ability in promoting differentiation was found in 3.2 mmol·L-1 DCA group and 1× 10-6 mol·L-1 ATRA group.The combination of DCA and ATRA had synergistic effect. Conclusion  Both DCA and ATRA can inhibit the proliferation and promote the differentiation of HL-60 cells, and the combination of DCA and ATRA had synergis? tic effect.

Key words: Decitabine, all-trans retinoic acid, HL-60 cell line, acute promyelocytic leukemia