天津医药

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山茱萸多糖对HeLa细胞的抑制作用及相关凋亡抑制蛋白表达的影响*

靳祎1,王恩军2   

  1. 1. 保定市裕华东路342号河北大学医学部基础医学院生化教研室
    2. 河北大学医学部
  • 收稿日期:2012-04-23 修回日期:2012-11-05 出版日期:2013-03-15 发布日期:2013-03-15
  • 通讯作者: 靳祎

The Effects of Fructus Corni Polysaccharides on the Proliferation of Human Cervical Cancer Cells and Expressions of Survivin and Bcl-2

  • Received:2012-04-23 Revised:2012-11-05 Published:2013-03-15 Online:2013-03-15

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摘要：

【摘要】目的 探讨山茱萸多糖对人宫颈癌HeLa细胞体外增殖的抑制作用及对相关凋亡抑制蛋白Survivin和 Bcl-2表达的影响。方法 采用MTT法、流式细胞术及免疫组化SP法检测山茱萸多糖对HeLa细胞抑制作用及Sur? vivin和Bcl-2蛋白表达的变化。结果 山茱萸多糖对HeLa细胞抑制作用明显，抑制程度与浓度、时间相关；6.25 g/L、 12.5 g/L、25 g/L的山茱萸多糖对HeLa细胞的凋亡率分别为10.1%、21.6%和28.9%，明显高于对照组的1.2%。山茱萸多糖致使HeLa细胞凋亡抑制蛋白Survivin和Bcl-2的表达量降低。结论 山茱萸多糖对HeLa细胞增殖有较强的抑制作用，能够降低Survivin和Bcl-2的表达，诱导细胞凋亡。

关键词: 山茱萸多糖, 宫颈癌, Hela, Survivin, Bcl-2, 抑制作用, 凋亡

Abstract:

[Abstract] Objective  To explore the inhibitory effect of fructus corni polysaccharides on human cervical cancer cells and expressions of Survivin and Bcl-2 in HeLa cells. Methods  MTT assay was used to determine the cell proliferation. Flow cytometry and immunohistochemistry SP methods were used to detect the apoptosis and protein expressions of Survivin and Bcl-2 in HeLa cells. Results  There was a significant inhibitory effect of fructus corni polysaccharides on the proliferation in HeLa cells. The inhibitory degree was correlated with different concentrations and different action times of fructus cornipolysaccharides. The apoptosis rates of HeLa cells were 10.1%, 21.6% and 28.9% for fructus corni polysaccharides concentrations of 6.25 g/L, 12.5 g/L and 25 g/L. The expressions of Survivin and Bcl-2 in HeLa cells were significantly lower in fructus corni polysaccharides treatment group than those of control group. Conclusion  Fructus corni polysaccharides showed significant inhibitory effects on the proliferation in HeLa cells, which also decreased Survivin and Bcl-2 expressions and induced the cell apoptosis.

Key words: Fructus Corni poLysaccharides, Cervical Cancer, Hela, Survivin, Bcl-2, inhibitory, Apoptosis