天津医药

• 临床论丛 •    

甲胎蛋白异质体在鉴别良恶性肝病中的应用价值

丁贤   

  1. 天津市第三中心医院
  • 收稿日期:2010-03-04 修回日期:2010-05-19 出版日期:2010-09-15 发布日期:2010-09-15
  • 通讯作者: 丁贤

Applicated value of serum AFP-L3 variants assay for differential diagnosis of liver disease

DING Xian   

  • Received:2010-03-04 Revised:2010-05-19 Published:2010-09-15 Online:2010-09-15
  • Contact: DING Xian

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摘要： 摘要 目的: 探讨甲胎蛋白异质体(AFP-L3)在鉴别良恶性肝病中的应用价值。方法: AFP-L3的分离技术采用亲和微量离心柱法,AFP和AFP-L3含量测定采用电化学发光法（ECLIA）,分别对241例AFP阳性肝病患者进行检测。 结果:以AFP-L3/AFP＞10%为AFP-L3阳性判断标准，肝癌患者血清AFP-L3阳性率：AFP＞400ng/ml和AFP＜400ng/ml分别为79.0%和75.9%。肝硬化、慢性肝炎和自身免疫性性肝炎患者AFP-L3阳性率分别为8.7%、7.9%和7.3% 结论:AFP-L3在肝癌诊断和低浓度AFP良恶性肝病鉴别诊断中均有重要的临床应用价值。

关键词: 甲胎蛋白, 甲胎蛋白异质体, 亲和微量离心柱法, 电化学发光法

Abstract: Abstract 0bjective: To investigate the value of serum AFP-L3 variants assay for differential diagnosis of liver disease.Methods : serum AFP-L3 variants were isolated by micro centrifugal Column,and the contents of AFP and AFP-L3 were detected by Elcteochemo luminescence immuno assay(ECLIA). Result：When AFP-L3/AFP＞10% was used as a positive criterion ,in the cases of AFP＞400ng/ml and AFP＜400ng/ml, the positive rate of AFP-L3 were 79.0% And 75.9%, respectively in hepatocellular carcinoma(HCC), which in liver cirrhosis(LC)、chronic hepatitis(CH) and autoimmune hepatitis(AIH) were 8.7%、7.9% and 7.3% ,respectively.Conclusion ：AFP-L3 is very useful in the differential diagnosis of HCC and low AFP level liver disease.

Key words: AFP, AFP-L3, micro centrifugal Column, ECLIA