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纯钛种植体表面的飞秒激光制备及其短期植入研究

李春艳   

  1. 天津医科大学口腔医院
  • 收稿日期:2011-01-27 修回日期:2011-04-26 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 李春艳

The Surface Preparation on Pure Ti Implant Using Femtosecond Laser and Short-term Implants Research

chun-yan LI   

  • Received:2011-01-27 Revised:2011-04-26 Published:2011-09-15 Online:2011-09-15
  • Contact: chun-yan LI

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摘要： 摘要 目的:探讨新的种植体表面处理方法，以提高种植体-骨界面结合强度及促进早期骨结合。方法:应用飞秒激光处理浸没于HA过饱和液中的纯钛试样表面作为实验组，未处理组为对照组，用扫描电镜和能谱仪分析试样表面形貌及元素组成；将试样植入新西兰大白兔股骨4周后制作组织学切片来观察实验组试样的早期成骨性及骨诱导性。结果:扫描电镜下实验组试样表面具有纳米级的孔洞和微米级的珊瑚岛状、沟槽状结构组成的图案化结构，在珊瑚岛状突起中间和沟槽中可见与基体紧密结合的负载物，能谱分析显示试样表面主要由钛、钙及磷元素组成，与基体紧密结合的负载物为钙磷元素，对照组试样表面光滑。组织学结果显示，与对照组相比，实验组试样表面类骨质、钙磷盐的沉积更加活跃。结论:飞秒激光能在浸没于过饱和HA溶液中的试样表面形成多层图案化的拓扑结构，并同时负载Ca/P元素。与光滑表面试样相比，飞秒激光处理的纯钛试样早期成骨的速度快，有利于种植体的初期稳定性。

关键词: 飞秒激光, 动物实验, 组织学研究, 骨整合, 表面形貌

Abstract: Abstract Objective: To elucidate new implant surface treatment methods, in order to enhance bone implant interface bonding strength and promote initial osseointegration. Methods: Pure titanium specimen submerged in HA supersaturated liquid surface processed by femtosecond laser as the test group, untreated group as control group. Surface topography of the samples was examined by SEM and the element concentration of the samples was analyzed through EDX. The samples were implanted in New Zealand rabbits femoral. After 4 weeks, making histological sections to observe experimental samples of initial bone regeneration and osteoconduction. Results: It is found that three-stage hierarchical surface structures are produced on the surface of implants which was composed of grooves, islands, nanoholes under SEM. Remarkably, Ca/P deposition were revealed on the micro-structured surfaces firmly by EDX. The surface of the control group was smooth. Histological results showed the formation of osteoid and the deposition of calcium phosphate in the text group were more active than the control group. Conclusion: The femtosecond lasers can induced topo patterns surface on pure Ti surface in HA suspension and promoted Ca/P element to deposited on the micro-patterns. Contrast with the control group, the samples processed by femtosecond laser were benefit to bone regeneration and initial stabilization.

Key words: femtosecond laser, animal experiment, histology study, osseointegration, surface topography