天津医药

• 实验研究 •    

线形程序性坏死在不同肿瘤局部微环境中分布的初步观察

刘艳荣1,古强2,张丹芳2,孙涛2,张诗武2,赵秀兰2,韩春荣2,孙保存3   

  1. 1. 天津医科大学病理教研室
    2.
    3. 天津医科大学
  • 收稿日期:2009-11-11 修回日期:2009-11-23 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 刘艳荣

The Pilot Study On The Influence Of The Local Tumor Microenvironments On LPPCN Formation

  • Received:2009-11-11 Revised:2009-11-23 Published:2010-04-15 Online:2010-04-15

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摘要： 摘要 目的：观察肿瘤局部微环境对肿瘤细胞线形程序性坏死(linearly patterned programmed cell necrosis ,LPPCN)发生的影响。方法：C57BL小鼠9只，结扎小鼠左后肢股动脉，注射B16 单细胞悬液于左右后肢（右后肢为对照组），构建B16F10小鼠黑色素瘤后肢缺血模型；C57BL小鼠10只，注射B16 单细胞悬液于小鼠腹腔与左后肢，构建小鼠黑色素瘤生物力学模型；HE切片观察不同组间LPPCN分布的差别；免疫组织化学染色检测肿瘤组织基质金属蛋白酶（MMP）-2、MMP-9的表达。结果：缺血组和后肢组LPPCN细胞百分率分别高于对照组和腹腔组，差异均有统计学意义（均P<0.01）；缺血组和后肢组MMP-2、MMP-9阳性细胞百分率分别高于对照组和腹腔组，差异均有统计学意义（均P<0.01）。结论：肿瘤局部缺氧和压力变化可以上调MMP-2、MMP-9的表达并诱导肿瘤细胞发生LPPCN。

关键词: 黑色素瘤, 线形程序性坏死, 动物模型, 缺氧, 肿瘤血管生成

Abstract: Abstract: Objective: To study the influence of the local tumor microenvironments on LPPCN formation(linearly patterned programmed cell necrosis, LPPCN). Methods: The left femoral arteries of 9 C57BL mice were ligated and then B16F10 melanoma cells suspension was injected into the left ischemic skeletal muscles and non- ischemic right controls respectively to build the ischemic animal model; Biomechanical animal model was built by injecting the B16F10 melanoma cells suspension into the the abdominal cavity and left skeletal muscles of 10 C57BL mice.HE staining was performed to compare the LPPCN distribution among groups.Immunohistochemistry was employed to detect the expression of matrix metalloproteinase-2 & 9 .Results: The LPPCN cell numbers in the ischemic group were significantly higher than those in the control group （P<0.01）； and the skeletal muscle group significantly higher than those in the abdominal cavity group（P<0.01）.Similarly,the expression of MMP-2 and MMP-9 was higher in the ischemic group than that in the control group （P<0.01）and the skeletal muscle group significantly higher than those in the abdominal cavity group（P<0.01）.Conclusion:Hypoxia and microenvironment pressure of tumor can increase MMP-2, MMP-9 expression and influence the LPPCN formation.

Key words: Melanoma, LPPCN, Animal model, Hypoxia, Tumor angiogenesis