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日本血吸虫感染小鼠CD8α-CD11c+型树突状细胞对哮喘抑制作用的肺部病理学研究

路平,刘佩梅   

  1. 天津医科大学寄生虫教研室
  • 收稿日期:2011-12-20 修回日期:2012-03-19 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 路平

The inhibitory effect on asthma by CD8α-CD11c+ dendritic cells from Schistosoma japonicum infected mice

  • Received:2011-12-20 Revised:2012-03-19 Published:2012-07-15 Online:2012-07-15

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摘要： 摘 要：目的 通过过继转移日本血吸虫感染鼠CD8α-CD11c+树突状细胞(DC) ，研究CD8α-CD11c+DC在抑制过敏性哮喘中的作用。方法 用磁珠分选（MACS）方法纯化日本血吸虫感染鼠及正常鼠DC亚群CD8α-CD11c+细胞。将20只BALB/c小鼠随机分为4组，A组为过继转移日本血吸虫感染鼠CD8α-CD11c+ DC并诱发过敏性哮喘组，B组为过继转移正常鼠CD8α-CD11c+ DC并诱发过敏性哮喘组，C组为单纯过敏性哮喘组，D组为生理盐水对照组。A、B两组小鼠经尾静脉过继转移5 ×105 DC，1h后A、B、C3组均开始诱发哮喘。4周后处死小鼠，取小鼠肺组织进行病理学检查。 结果 与B、C 组比较，A组肺部炎症明显减轻， 按Underwood标准，A、B、C 、D 4组总评分分别为7.67±2.34、l0.86±1.35、11.75±2.22、4.5±1.73 ，差异有统计学意义( P<0．05)。结论 日本血吸虫感染鼠CD8α-CD11c+D C明显抑制过敏性哮喘的发生。

关键词: 日本血吸虫, 过敏性哮喘, 树突状细胞, 过继转移

Abstract: Abstract: Objective To study the inhibitory effect on allergic asthma by adoptive transfer of 　　　CD8α-CD11c+ dendritic cells (DC) from Schistosoma japonicum-infected mice． Methods CD8α-CD11c+DC from S．japonicum-infected mice and naive were isolated and purified using microbeads respectively，. Twenty BALB/c mice were divided into four groups randomly．5×105 CD8α-CD11c+DCs from S．japonicum-infected mice were injected into the tail vein of each mouse in Group A，while 5×105 CD8α-CD11c+ DCs of na?ve were injected into the tail vein of each mouse in Group B. One hour after injection，mice in Group A、B and C were sensitized and challenged with ovalbumin to induce asthma．Four weeks later，all the mice were killed，and the left lungs were cut for histopathologica1 analysis．Results Compared with mice in Group B and C，pulmonary inflammation of mice in Group A diminished markedly．According to Underwood’s standard，average total scores of Group A，B，C and D were 7.67±2.34，l0.86±1.35，11.75±2.22and 4.5±1.73 respectively ( P< 0.05)． Conclusion CD8α-CD11c+ DC from S．japonicum infected mice can significantly inhibit allergic asthma．

Key words: Schistosoma japonicum, asthma, dendritic cells, adoptive transfer