天津医药

• 论著 •    

影响人囊胚形成因素的研究

鲁振宇1,张云山1,糜若然2   

  1. 1. 天津市中心妇产科医院生殖医学中心
    2. 天津医科大学总医院
  • 收稿日期:2009-07-10 修回日期:2009-11-23 出版日期:2010-04-15 发布日期:2010-04-15
  • 通讯作者: 鲁振宇

Factors affecting human blastocyst formation in vitro

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  • Received:2009-07-10 Revised:2009-11-23 Published:2010-04-15 Online:2010-04-15

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摘要： 摘要 目的：分析影响人囊胚形成的因素。方法：接受试管婴儿治疗的196位患者D3移植后剩余无冻存价值的406个胚胎，在患者签署知情同意书后进入本实验。将胚胎于D3转移至CCM中，每天观察胚胎发育的情况直至受精D5-7并且对囊胚进行分级。结果：共有102个囊胚形成，囊胚形成率为25.12%，优质囊胚有28个，占所有囊胚的27.45%，全部由Ⅱ+级以上胚胎形成。64位有囊胚形成的患者有32人获临床妊娠，临床妊娠率为50%，高于132位无囊胚形成患者28.79%的妊娠率（P<0.05）。结论：D3胚胎细胞数与囊胚形成率呈显著正相关关系，Ⅰ-Ⅱ+级胚胎比Ⅲ-Ⅳ级胚胎囊胚形成率、优质囊胚形成率高。剩余胚胎囊胚的形成对妊娠具有间接的预测意义。

关键词: 胚胎, 囊胚, 妊娠率

Abstract: Factors affecting human blastocyst formation in vitro LUZhenYu ZHANG Yun Shan Miruoran Tianjin Central Hospital of Gynecology & Obsterics, Tianjin 300052, China Abstract Objective: To investigate the factors affecting human blastocyst formation in vitro. Methods: Through sequential culturing the D3 discarded embryos to D5-7, we observed the blastocyst formation and detect the relationship between the D3 embryo cells number, quality grade and the blastosysts quality, the formation of the high-quality blastocysts, providing the academic basis for the blastocyst transplantation. 406 D3 discarded embryos entered this experiment. Cultured these embryos with CCM, observed theirs changes daily till D5-7. Results: 102 blastocysts were formed including 28 high-quality blastocysts using the blastocyst grade standard from Gardener. The blastulation rate was 25.12%. Among 64 patients who have blastocysts, 32 patients pregnancied, the pregnancy rate is higher than whose without blastocysts (50%Vs28.79%, P<0.05). Conclusion: The blastultions rate is positively correlative with the D3 embryo cells numbers. The blastulation rate of the embryos withⅠ-Ⅱ+ degree is high than those with Ⅲ-Ⅳ degree.

Key words: embryo, blastocyst, pregnancy rate