天津医药

• 论著 • 上一篇    下一篇

脂肪细胞型脂肪酸结合蛋白与妊娠期糖尿病的相关性研究

董胜雯1,韩姹1,高晓丽2,牛秀敏2,3   

  1. 1. 天津医科大学
    2. 天津医科大学总医院
    3.
  • 收稿日期:2011-04-28 修回日期:2011-05-09 出版日期:2011-07-15 发布日期:2011-07-15
  • 通讯作者: 董胜雯

Study on the correlations of adipocyte fatty-acid binding protein and gestational diabetes mellitus

  • Received:2011-04-28 Revised:2011-05-09 Published:2011-07-15 Online:2011-07-15

PDF (PC)

2133

摘要： 目的：本研究通过测定脂肪细胞型脂肪酸结合蛋白（FABP4）在妊娠期糖尿病（GDM）与正常妊娠妇女（NGT）血清和胎盘组织中表达及相互关系。方法：采用酶联免疫吸附试验和免疫组化方法检测GDM与NGT的正常组和超重组血清和胎盘组织中FABP4的表达，检测空腹血糖、血清胰岛素、甘油三酯和总胆固醇水平。采用稳态模型计算胰岛素抵抗指数（HOMAIR）、胰岛素敏感指数（HOMAISI）、胰岛β细胞功能（HOMA-B％），并统计分析相关资料。结果：1.与NGT正常组相比，GDM正常组孕前BMI、FBG、FINS、甘油三酯、总胆固醇、HOMAIR均增高（P＜0.05），HOMAISI、HOMA-B％均低于正常组（P＜0.05）。GDM孕妇中，超重组孕前BMI、FBG、FINS、甘油三酯、总胆固醇、HOMAIR均高于正常组（P＜0.05），HOMAISI、HOMA-B％均低于正常组（P＜0.05）。2.ELISA检测四组的OD值存在明显差异（P＜0.05）。3.GDM正常组胎盘组织中蛋白表达高于NGT正常组，差异有统计学意义（Ｚ=-3.312，P=0.002），GDM超重组FABP4表达高于GDM正常组，差异具有统计学意义（Ｚ=-2.663，P=0.013）。结论：脂肪细胞因子FABP4在GDM发生过程中起了重要的作用。

关键词: 妊娠期糖尿病, 胰岛素抵抗, 体质量, 肥胖, 脂肪细胞型脂肪酸结合蛋白

Abstract: Objective To study the expression of adipocyte fatty-acid binding protein FABP4 in placental tissues and serum of normal and diabetic pregnant women. The relationship among body weight， substances and the insulin resistance index was discussed，.Methods The expression of FABP4 was detected in serum and placental tissues of diabetic pregnant women and normal ones，which are respectively grouping to normal and overwight according to body weight，with the application of enzyme-lingked immunosorbent assay（ELISA）. The fasting blood glucose，insulin levels， triglyeride and total cholesterol were determined separately. The indexes of insulin resistance， insulin sensitivity index， β-cell function， were calculated with homeostasis model （HOMA）. All relevant information was statistical analyzed. Restult 1. The body mass index before pregnancy，fasting blood glucose，fasting insulin，triglyeride，total cholesterol，insulin resistance index of GDM normal group were higher than NGT normal group （P＜0.05）. While the insulin sensitivity index， β cell function of GDM group were lower than NGT normal group. In GDM pregnant women，BMI change value，FBG，FINS，triglycerides，total cholesterol，HOMAIR in the overweight group were higher than normal group （P＜0.05），HOMAISI， HOMA-B% are lower than the normal group. 2.The differences among the four groups of the OD dates were significant（P＜0.05）. 3.Compared with the control normal group，positive cells of GDM normal is higher.The difference was statistically significant（Ｚ=-3.312，P=0.002）. The positive cells of GDM overweight is higher the GDM normal.The difference was statistically significant（Ｚ=-2.663，P=0.013）. Conclusion The adipocytokine- FABP4 may play an important role in the process of GDM.

Key words: gestational diabetes, insulin resistance, body weight, obesity, adipocyte fatty acid binding protein