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冯润荷
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李晓倩 FENG
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冯润荷. 不同剂量过氧化氢对心肌游离线粒体Cyt-c释放的影响*[J]. .
李晓倩 FENG. Different dosage of hydrogen peroxide on myocardial mitochondria Cyt c - free release[J]. .
摘要： 摘要 目的：探索小剂量过氧化氢预处理可减少高钙诱导的细胞色素c释放，从而抑制mPTP的开放。方法：分离大鼠肝脏线粒体，检测不同浓度的Ca2+刺激游离线粒体Cyt-c，Smac释放量；检测不同剂量H2O2干预Cyt-c释放量。结果:小剂量H2O2预处理减少钙超载诱导的Cyt-c释放。结论：小剂量H2O2预处理减少钙超载诱导的mPTP形成与开放，通过抑制线粒体通透转换发挥心肌保护功效。
关键词: 过氧化氢, 细胞色素c, 钙超载, 心肌保护
Abstract: Abstract Objective: to explore small doses of hydrogen peroxide pretreatment can reduce high calcium induced cytochrome c release, thus inhibiting mPTP open. Method: separation of rat liver mitochondria, the detection of different concentrations of Ca2 + to stimulate free mitochondrial Cyt c, Smac release quantity; Detection of different dose of H2O2 intervention Cyt c release quantity. Results: a small dose of H2O2 preconditioning reduces calcium overload induced Cyt c release. Conclusion: small dose of H2O2 preconditioning reduces formation and open, the mPTP induced by calcium overload through inhibiting mitochondrial permeability transition myocardial protection effect into full play.
Key words: hydrogen peroxide, cytochrome c, calcium overload, myocardial protection
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https://www.tjyybjb.ac.cn/CN/Y2014/V42/I3/220
