天津医药

天津医药

• 细胞与分子生物学 • 上一篇    下一篇

SDF-1对糖尿病外周血EPCs功能影响及PI3K/AKT信号转导机制的研究

黎金凤,林安华,邓颖,霍亚南,刘精东,吴明斌,王晨秀   

  1. 江西省人民医院内分泌科
  • 收稿日期:2014-05-26 修回日期:2014-07-18 出版日期:2014-11-15 发布日期:2014-11-15
  • 通讯作者: 霍亚南

Effects of Stromal Cell-Derived-Factor-1on Endothelial Progenitor Cells of Peripheral Blood and Their Relationship with PI3K/AKT Signal Transduction Pathway in Patients with Diabetes

LI Jin feng,LIN An hua,DENG Ying ,HUO Ya’ nan,LIU Jing dong,WU Ming bin,WANG Chen xiu   

  1. Department of Endocrinology,The People's Hospital in Jiangxi Province
  • Received:2014-05-26 Revised:2014-07-18 Published:2014-11-15 Online:2014-11-15
  • Contact: HUO Ya’ nan

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摘要：

【摘要】  目的  观察基质细胞衍生因子-1（SDF-1）对糖尿病外周血内皮祖细胞（EPCs）功能的影响，探讨SDF-1对EPCs的影响是否与PI3K/AKT信号通路有关。  方法  采集糖尿病患者（DM组）和健康对照者（HC组）外周血30mL，提取并培养EPCs。（1）SDF-1干预组加入100 μg/L SDF-1培养液，非干预组加入EGM-2MV培养基，采用Boyden小室和体外血管生成试剂盒观察EPCs的迁移和体外血管生成能力。（2）将培养的EPCs分为空白对照组、1μg/L SDF-1组、10μg/L SDF-1组、100μg/LSDF-1组、单纯AMD3100组及100μg/L SDF-1+AMD3100组，通过Western blot法检测各组EPCs中AKT蛋白的表达水平。  结果  （1）无SDF-1干预时，DM组EPCs迁移和血管形成能力低于HC组，SDF-1干预后，2组的EPCs迁移和血管形成能力均较干预前增强，但DM组增强的幅度高于HC组。（2）同一浓度下，DM组的AKT蛋白表达水平均低于HC组（均P＜0.01）。无论是DM组还是HC组，AKT蛋白的表达均随着加入SDF-1浓度的增加而呈递增趋势（P＜0.05）；100μg/L SDF-1+AMD3100组AKT蛋白的表达水平较100μg/L SDF-1组明显降低（P＜0.05）。  结论   SDF-1可增强外周血EPCs迁移和血管形成能力，对糖尿病患者效果更为明显，且SDF-1对EPCs的影响与PI3K/AKT信号通路有关。

关键词: 基质细胞衍生因子-1;内皮祖细胞, PI3K/AKT;糖尿病

Abstract:

[Abstract]   Objective  To observe the effects of stromal cell-derived-factor-1(SDF-1) on the function of endothelial progenitor cells（EPCs）of peripheral blood in patients with diabetes, and to discuss the effects of PI3K/AKT signaling path?way on the role of SDF-1in EPCs. Methods   The peripheral blood samples (30mL) were collected in10diabetes patients (DM group) and 10healthy controls (HC group). (1) The 100μg/L SDF-1was added in intervention group. EGM-2MV was added in non-intervention group. The Boyden chamber and in vitro angiogenesis kit were used to analyze the migration and in vitro angiogenesis of EPCs. (2) Cultured EPCs were divided into blank control group, 1μg/L SDF-1group,10μg/L SDF-1group,100μg/L SDF-1group, pure AMD3100group and100μg/L SDF-1+AMD3100group. AKT protein expression lev?els of endothelial progenitor cells were detected by Western blot assay in each group.  Results   (1) Without intervention with SDF-1, EPCs’migration and angiogenesis ability were lower in DM group than those in HC group. After intervention with SDF-1, the migration and angiogenesis ability were enhanced in two groups, but the increased level was higher in DM group than that of HC group. (2) Under the same concentration, AKT protein expression level was significantly lower in DM group than that in HC group (P<0.01). AKT protein expression levels were increased with the increased levels of SDF-1in DM group and HC group (P<0.05). AKT protein expression was significantly lower in 100μg/L SDF-1+AMD3100group than that of 100μg/L SDF-1group (P<0.05). Conclusion   SDF-1can increase the chemotactic migration and angiogenesis ability of EPCs in peripheral blood, especially for patients with diabetes. The effects of SDF-1on EPCs were related to the PI3K/AKT signaling pathway.

Key words: Stromal cell-derived-factor-1, Endothelial progenitor cells, Phosphoinositide3-kinase /protein kinase B, Diabetes