天津医药

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L-丙氨酸对INS-1E细胞胰岛素分泌功能的影响

刘振平1,申晶2,宋晓艳2   

  1. 1. 解放军总参谋部总医院（解放军第309医院）
    2.
  • 收稿日期:2010-01-11 修回日期:2010-09-11 出版日期:2011-02-15 发布日期:2011-02-15
  • 通讯作者: 刘振平

The effect of L-alanine on the Insulin Secretion of clonal beta cell INS-1E Cell

  • Received:2010-01-11 Revised:2010-09-11 Published:2011-02-15 Online:2011-02-15
  • Contact: ZHENPING LIU

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摘要： 摘要：目的：探讨L-丙氨酸对INS-1E细胞分泌胰岛素的刺激作用及其葡萄糖依赖性。材料与方法：INS-1E细胞经传代培养两天后，在Krebs-Ringer缓冲液中37°C培养箱预培养30分钟，再用不同浓度葡萄糖和不同浓度L-丙氨酸的改良Krebs-Ringer缓冲液培养60分钟，然后留取上清液进行胰岛素测定。结果：L-丙氨酸在0.1-20mM范围促进了葡萄糖诱导的INS-1E细胞的胰岛素分泌，随剂量增大而增强；在1.1mM--25mM 葡萄糖范围内，随着葡萄糖浓度的增加，INS分泌量逐渐增加。在10mM L-丙氨酸存在的条件下，在1.1mM/3.3 mM 葡萄糖的情况下未显示增加胰岛素分泌的作用；在6.7mM--25mM葡萄糖范围内，随着葡萄糖浓度的增加，INS分泌量也显示逐渐增加的趋势。结论：本研究显示L-丙氨酸能增加葡萄糖诱导的INS-1E细胞的胰岛素分泌，此作用依赖于一定水平的葡萄糖，随着葡萄糖浓度的增加，INS分泌量逐渐增加。

关键词: L-丙氨酸, 胰岛B细胞, 胰岛素释放

Abstract: Abstract: Objective: To explore the dose- and glucose-dependency of L-alanine induced insulin secretion from INS-1E cells. Materials and Methods: Passage INS-1E cells incubated in the RPMI 1640 for 2 days, then the cells were incubated in the Krebs-Ringer buffer(KRB) with 0.1%BSA for 30 minutes. After preincubation, the cells were incubated for 60 minutes in the modified KRB containing glucose and alanine according to the protocol. After the incubation the medium was collected for insulin assay. Results: Alanine at concentration of 0.1-20mM potentiated the glucose (16.7mM) induced insulin secretion in a concentration dependent manner with a maximal effect obtained at 20mM. Glucose at concentration of 1.1mM--25mM enhanced insulin secretion in a concentration dependent manner with a maximal effect obtained at 25mM. 10mM L-alanine with 1.1mM—3.3mM glucose did not enhance insulin secretion; 10mM L-alanine with 6.7mM--25mM glucose enhanced insulin secretion in a glucose concentration dependent manner and the maximal effect obtained at 10mM L-alanine plus 25mM glucose. Conclusion: The present study shows that L-alanine can enhance glucose-induced insulin secretion of INS-1E cells, The higher glucose, the more insulin secreted.

Key words: L-alanine, beta-cell, Insulin release