天津医药

• 细胞与分子生物学 • 上一篇    下一篇

微小RNA-1在缺氧心肌细胞凋亡中的作用

郑君毅1,王霁翔1,肖建勇1,丛洪良2   

  1. 1. 天津市胸科医院
    2. 天津胸科医院
  • 收稿日期:2013-08-26 修回日期:2014-01-09 出版日期:2014-07-15 发布日期:2014-07-15
  • 通讯作者: 郑君毅

Apoptotic Effects of MicroRNA-1on Hypoxemic Cardiomyocytes

  • Received:2013-08-26 Revised:2014-01-09 Published:2014-07-15 Online:2014-07-15

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摘要：

【摘要】目的 研究微小RNA（miR）-1在缺氧心肌细胞凋亡中的作用。方法 将培养的H9C2心肌细胞分为5 组，正常对照组、阴性对照组、H2O2组、miR-1组和H2O2+miR-1组。用实时荧光定量PCR方法证实miR-1转染成功后，MTT法和流式细胞术检测细胞存活率和凋亡情况，用PCR和Western Blot的方法检测抗凋亡因子bcl-2的mRNA 和蛋白表达情况。结果与正常对照组相比，阴性对照组各个指标的变化差异均无统计学意义。其他3组细胞的 miR-1表达水平显著升高，细胞存活率下降，凋亡率增加，Bcl-2的mRNA和蛋白表达水平均明显降低。结论 微小 RNA-1可通过降低抗凋亡因子Bcl-2的表达水平诱导缺氧心肌细胞的凋亡。

关键词: 缺氧, 心肌细胞, 细胞凋亡, 微小RNA, miR-1

Abstract: [Abstract] Objective To investigate the apoptotic effect of microRNA-1(miR-1) on hypoxemic cardiomyocytes. Methods The cultured H9C2cells were divided into5groups: normal control group, negative control group, H2O2group, miR-1group and H2O2+miR-1group. After verified the success of transfection by real time PCR, MTT and flow cytometry methods were used to test the cell vitality and apoptotic rate, while the mRNA and protein expression level of Bcl-2were de? tected by real time PCR and Western blot methods. Results Compared with normal control group, there were no significant differences in all indexes in negative control group. The application of H2O2 and miR-1respectively or together significantly increased the miR-1level and apoptotic rate, and reduced the cell vitality and Bcl-2expression level.Conclusion mi? croRNA-1can induce cardiomyocyte apoptosis by downregulating anti-apoptosis factor Bcl-2.

Key words: hypoxemic, cardiomyocyte, cell apoptosis, microRNA, miR-1