天津医药

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二黄汤对哮喘大鼠气道重塑中转化生长因子-β1及体内白介素-33表达的影响

聂艳辉   

  1. 辽宁医学院
  • 收稿日期:2013-07-11 修回日期:2013-12-02 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 聂艳辉

The Effect of Erhuang Decoction on Transforming Growth Factor-β1of Lung Tissues and the Expression of Leukotriene-33in Asthmatic Rats

yanhui NIE   

  • Received:2013-07-11 Revised:2013-12-02 Published:2014-04-15 Online:2014-04-15
  • Contact: yanhui NIE

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摘要： 摘要 目的：观察二黄汤对哮喘大鼠气道重塑中转化生长因子β1(TGF-β1)及体内白介素-33(IL-33)的影响。方法：50 只SD大鼠随机均分为正常组（A）、哮喘组（B）、布地奈德组（C）、高剂量二黄汤组（D）和低剂量二黄汤组（E）。以卵清白蛋白（VOA）致敏与激发建立哮喘大鼠气道重塑模型。用免疫组化法检测肺组织TGF-β1蛋白的表达，酶联免疫法检测血清及肺泡灌洗液（BALF）中IL-33的浓度。结果：与A组比较，B组支气管壁厚度（Wat）、平滑肌厚度（Wam）、血清、BALF中IL-33的浓度和肺组织中TGF-β1的蛋白表达均增高（P<0.05）。经布地奈德和二黄汤干预后，与B组比较，C组、D组和E组以上指标均下降（P<0.05）；与C组比较，C组、D组各检测结果无差异（P>0.05）,C组、E组各检测结果有差异，但不显著（0.01

关键词: 气道重塑, 二黄汤, 转化生长因子-β1, 白介素-33

Abstract: [Abstract] Objective To investigate the effect of Erhuang decoction on TGF-β1expression of lung tiusses and the concentrations of IL-33in asthmatic rats. Methods Fifty Sprague- Dawley rats were randomly divided into five groups equally: Control group, Asthmatic group, Budesonide aerosol group, High-dose Erhuang decoction group (68g/kg)and Low dose Erhuang decoction group(17g/kg). The model of asthma was established by ovalbumin (OVA) sensitizing and challeng? ing. Then Erhuang decoction and budesonide aerosol was used respectively for intervention therapy. Histologic HE staining were used to observe the general pathologic alteration and to analyze the total bronchial wall area (Wat) and the muscle wall area(Wam). The protein expressions of TGF-β1 in the lung tissues were detected by immunohistochemistry. The concentra? tions serum IL-33 and BALF were tested by sandwich ELISA. Results There was significant reduction in the infiltrated inflammatory cells in all drug intervention groups compared with asthma group; The Wat and Wam in asthmatic group was significantly higher in than those in Budesonide aerosol group，High-dose Erhuang decoction group and Low-dose Erhuang decoction group ( Watμm 2 /μm :54.99±8.82, 52.28±7.61, 58.53±7.63vs79.50±5.64, P＜0.05; Wam μm 2 /μm :22.74±2.73, 20.63±1.72, 21.20±4.50vs 30.16±1.68, P＜0.05); Compared with control group, BALF and serum IL-33concentration were significantly higher in asthmatic group. Compared with asthmatic group, all the indicators were significantly decrease in the treatment groups after drug intervention (P＜0.05). Andthere was no significant difference between the treatment groups in all the indicators. TGF-β1expression in lung tissues in asthmatic group were significantly higher than that in control group (12.60±2.25vs1.67±0.17). Compared with asthmatic group, there was significantly reduction of TGF-β1 expression in the Budesonide aerosol group (5.51±2.48), High-dose Erhuang decoction group (5.22±2.52) and Low-dose Erhuang decoction group (6.92±2.18) (P＜0.05). There were no significant difference between the treatment groups. TGF-β1 expression and se? rum IL-33concentration in asthmatic rats were positively correlated with Wat and Wam.Conclusion The effects of Er? huang decotion on ameliorating the progression of airway remodeling about asthmatic rats may be partially by regulating TGF-β1and IL-33.