天津医药

• 论著 •    

肝细胞癌患者外周血CD95和OX40L的表达

李克秋1,王玉亮2,苗绪红3,李健3,李光4   

  1. 1. 天津医科大学基础医学院生物学教研室
    2. 天津市第一中心医院
    3.
    4. 天津医科大学医用生物学教研室
  • 收稿日期:2009-12-30 修回日期:2010-01-13 出版日期:2010-03-15 发布日期:2010-03-15
  • 通讯作者: 李克秋

Expression of CD95 and OX40L in hepatocellular carcinoma

  • Received:2009-12-30 Revised:2010-01-13 Published:2010-03-15 Online:2010-03-15

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摘要： 目的:研究肝细胞癌（HCC）患者外周血CD95和OX40L mRNA的表达并探讨其临床意义。 方法：用双色流式细胞术定量分析T淋巴细胞表面CD95+CD3+ 的表达量，采用荧光定量逆转录聚合酶链反应（RT-PCR）方法检测共刺激分子OX40L mRNA的表达水平。结果 ：HCC患者外周血CD95+CD3+的表达水平(34±20%)明显高于健康对照组(20±7%) （P <0.05）。 HCC患者外周血OX40L mRNA表达水平(0.46±0.36)明显低于健康对照组(0.86±0.70) （P <0.05）。结论：CD95及OX40L分子的异常表达在HCC的发生中起重要作用。

关键词: 肝细胞癌, CD95, OX40L, mRNA

Abstract: Aim: To assess the effects of the expression of CD95 and OX40 ligand (OX40L) messenger RNA (mRNA) in hepatocellular carcinoma (HCC), and to explore the clinical values. Methods: CD95 expression by CD3 positive T cells was quantitatively measured by dual color flow cytometry. Expression of OX40L mRNA were detected by TaqMan real-time reverse transcriptase- polymerase chain reaction (RT-PCR). Results: CD95 expression by CD3 positive T cells was significantly higher in patients with HCC (34±20%) compared with normal controls (20±7%) (P<0.05). The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with normal controls (P<0.05). Conclusion: These results suggest that abnormal expressions of CD95 and OX40L in patients with HCC play an oncogenic role at stage of human hepatocarcinogenesis.

Key words: Hepatocellular carcinoma, CD95, OX40L, mRNA