天津医药

• 实验研究 •    

奥扎格雷对大鼠肢体缺血再灌注所致肺损伤的保护作用

孟庆春1,王玲玲2,孔小燕3   

  1. 1. 河北秦皇岛港口医院 急诊科
    2. 河北秦皇岛港口医院
    3. 河北联合大学基础医学院病理生理学教研室
  • 收稿日期:2011-04-01 修回日期:2011-07-10 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 孔小燕

The Protective Effect of Sodium Ozagrel Lung Ischemia-Reperfusion Injury in Rat

  • Received:2011-04-01 Revised:2011-07-10 Published:2012-01-15 Online:2012-01-15

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摘要： 目的：探讨奥扎格雷钠(Ozagrel sodium)对大鼠肢体缺血再灌注后肺损伤的影响。方法：将60只雄性SD大鼠随机分成正常对照组、缺血再灌注组和奥扎格雷组，建立大鼠肢体缺血再灌注损伤模型。利用光镜和电镜观察肺组织形态学改变和超微结构改变，利用竞争放射免疫分析法测定血浆血栓素A2（TXA2)代谢产物血栓烷素B2(TXB2)和前列环素(PGI2)代谢产物6-酮-前列腺素F1a(6-Keto-PGF1a)，比色法测定肺组织中丙二醛（MDA）、超氧化物歧化酶(SOD)、黄嘌呤氧化酶（XOD），双抗体夹心ABC-ELISA法测定肺组织中白细胞介素(IL)-8、肿瘤坏死因子(TNF )-α，并进行比较。结果：与缺血再灌注组相比，奥扎格雷组肺血管扩张、炎性细胞聚集、组织水肿等均减轻，线粒体水肿、空泡变性，内膜下基底膜水肿、内皮细胞肿胀等情况明显减轻；血浆TXB2和6-keto-PGFla含量显著降低；肺组织MDA、XOD活力明显降低，SOD活力升高，IL-8、TNF-α水平均明显降低。结论：奥扎格雷钠可减轻大鼠肢体缺血再灌注后肺部的损伤。

关键词: 肺, 缺血再灌注, 白介素-8, 肿瘤坏死因子-α, 奥扎格雷钠, 大鼠

Abstract: Objective:To investigate protective effect of Ozagrel sodium on limb ischemia-reperfusion injury of lung in rats.Methods:60 SD rats were randomly divided into 3 groups:the normal control group,the limb-ischemia-reperfusion group,the Ozagrel sodium group. The model rats which underwent 4 hours ischemia and 2 hours reperfusion of hind limbs were made.The histological and ultra-structure changes were observed under light microscope and electron microscope, respectively. TXB2 and PGI2 levels in serum were separately measured by radio-immunoassay. We observed changes of MDA SOD XOD in lung tissue of different groups by colorimetry and observed changes of IL-8 and TNF-α in lung tissue of different groups by avidinbiotincomplex-ELISA. Results: Compared with the limb-ischemia-reperfusion group, The damage in lung tissues of the Ozagrel sodium group alleviate under optical microscopy and electron microscope.The levels of TXB2 and 6-keto-PGFla in serum decreased very significantly. The level of SOD was increased during reperfusion and the values of XOD and MDA were all decreased significantly. The levels of IL-8 and TNF-α in lung tissue were much lower. Conclusions: Ozagrel sodium can alleviate limb ischemia-reperfusion injury of lung in rats.

Key words: lung, ischemia reperfusion, IL-8, TNF-α, Ozagrel, rats