天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (7): 892-895.doi: 10.11958/20150122

• 临床研究 • 上一篇    下一篇

hsa-mir-144在食管鳞癌中的表达及临床意义

刘尚国 秦秀广 赵宝生 王天云 齐博 李汉臣   

  1. 1 新乡医学院第一附属医院胸外科（邮编 453100）；2新乡医学院
  • 收稿日期:2015-09-02 修回日期:2016-02-03 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 赵宝生 E-mail:liushangguo@xxmu.edu.cn
  • 作者简介:刘尚国（1969）， 男， 硕士， 主要从事食管癌疾病的研究
  • 基金资助:
    新乡医学院重点研究领域招标课题资助项目（ZD2011-8）

The expression and clinical significance of hsa-miR-144 in esophageal squamous cell carcinoma

LIU Shangguo, QIN Xiuguang, ZHAO Baosheng, WANG Tianyun, QI Bo， LI Hanchen   

  1. 1 Department of Thoracic Surgery, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang 453100, China; 2 Xinxiang Medical University
  • Received:2015-09-02 Revised:2016-02-03 Published:2016-07-15 Online:2016-07-15
  • Contact: ZHAO Baosheng E-mail:liushangguo@xxmu.edu.cn

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摘要： 目的 检测hsa-mir-144在食管鳞癌中的表达，探讨其与食管鳞癌病理特征及预后的关系。方法 采用实时荧光定量逆转录聚合酶链反应(RT-qPCR)法，对46例食管鳞癌及癌旁正常组织中的hsa-mir-144进行检测。结合随访资料，采用Kaplan-Meier法进行生存分析。结果 相对内参U6，hsa-mir-144在食管鳞癌组织中的表达量0.97(0.22–24.48)×10-6低于癌旁正常组织中的表达量8.60(0.089–258.2)×10-6，差异有统计学意义(Z= –2.221，P<0.05)。hsa-mir-144表达与食管鳞癌的淋巴结转移(Z= –2.758，P<0.05)、病理分期明显相关(Z=7.737，P<0.05)，而与性别、年龄、肿瘤大小、肿瘤部位、肿瘤分化程度、肿瘤浸润深度无明显相关(P均＞0.05)。生存分析显示，hsa-mir-144表达、淋巴结转移、肿瘤浸润深度、病理分期为食管鳞癌预后影响因素(P均<0.05)。结论 hsa-mir-144在食管鳞癌组织中表达下调，与食管癌发生、发展及预后相关，可作为新的食管癌分子生物标记物。

关键词: hsa-mir-144, 实时聚合酶链反应, 食管鳞癌

Abstract: Objective The aim of this study was to detect the of hsa-mir-144 in esophageal squamous cell carcinoma, and to investigate the relationship between clinicopathological features and prognosis. Methods RT-qPCR(Real-time quantitative reverse transcription polymerase chain reaction) method was used to detect the of hsa-mir-144 in 46 esophageal squamous cell carcinoma tissue and adjacent normal tissue. Combining with the clinical follow-up data, Kaplan–Meier method was used to analyze the survival．Results Comparing with internal reference U6，the amount 0.97(0.22–24.48)×10-6 of hsa-mir-144 in esophageal squamous cell carcinoma was lower than that 8.60(0.089–258.2)×10-6 in adjacent normal tissue, the difference was statistically significant (Z= –2.221, P<0.05). Expression of hsa-mir-144 was correlated with the lymph node metastasis (Z= –2.758，P<0.05)and pathologic stage(Z=7.737，P<0.05)，and it was not correlated with the sex, age of the patients and the size, location, differentiation degree, invasion depth of the tumor (all P＞0.05). Survival analysis indicated that lymph node metastasis, invasion depth of the tumor and pathologic stage were the influencing factors of prognoses (all P<0.05), but the of hsa-mir-144 was not correlated with the prognoses(P＞0.05). Conclusion The of hsa-mir-144 was lower in esophageal squamous cell carcinoma tissue, there was significant influence on the occurrence， development and prognosis of esophageal squamous cell carcinoma，it may serve as a new molecular marker.

Key words: reverse transcriptase polymerase chain reaction, prognosis , factor analysis , statistical , hsa- miR- 144, esophageal squamous cell carcinoma