天津医药

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代谢综合征未成年鼠肝脏5α-R1表达及其对肾上腺皮质功能的调控

魏莹1,刘戈力2,杨箐岩1,郑荣秀1,王彤3,鲍鹏丽1,杨林洁1   

  1. 1. 天津医科大学总医院
    2. 天津医科大学总医院儿科
    3. 天津医科大学代谢病医院
  • 收稿日期:2010-06-24 修回日期:2010-07-28 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 刘戈力

The study for 5α-R1 and adrenal cortex function in juvenile rat with metabolic syndrome

  • Received:2010-06-24 Revised:2010-07-28 Published:2010-10-15 Online:2010-10-15

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摘要： [摘要]目的:探讨代谢综合征未成年鼠肝脏中皮质醇代谢关键酶5α-还原酶1表达及其对肾上腺皮质功能的影响。方法: 方法:50克左右雄、雌性SD大鼠随机分为3组:普通饮食组(NC组);高脂组(FC组);高脂十高盐组(FSC组)。实验四周末测体重、体长、腹围、血压，观测内脏脂肪重量、血脂、血皮质醇、促肾上腺皮质激素释放激素等，行口服葡萄糖耐量试验及胰岛素释放试验。取肾上腺称重，取新鲜肝组织，测5α-还原酶1mRNA表达水平。结果: 高脂高盐饮食使未成年鼠体重、腹围、血压、内脏脂肪、血糖、胰岛素水平均比普通饮食组显著增加,血脂紊乱加重并表现出显著的胰岛素抵抗。该组肝脏5α-还原酶1 mRNA增加，并与肾上腺重量、血皮质醇及促肾上腺皮质激素释放激素水平显著相关。 结论: 我们成功以高脂高盐饮食诱导了代谢综合征未成年鼠模型，肝脏组织高表达5α-还原酶1可正性调控肾上腺皮质功能。提示针适时适当干预5α-还原酶1表达和活性可能是代谢综合征干预策略之一。

关键词: 高脂高盐饮食, 代谢综合征, 未成年鼠, 5α- 还原酶1, 肾上腺皮质功能

Abstract: ABSTRACT Objective: To investigate the Gene expression of hepatic 5α-reductase type 1 (5α-R1)and its effects for adrenal cortex in juvenile rat with metabolic syndrome. Methods: 50 grams of male, female SD rats (3 weeks, just weaned) were randomly divided into 3 groups, 12-14 animals in each group, were given routine diet (NC) and high fat diet(FC) and high fat-salt diet (FSC). Then the body weight,, body length, abdominal circumference, blood pressure, visceral fat weight, plasma lipids, plasma cortisol and corticotropin-releasing hormone were measured 4 weeks later, at the same time oral glucose tolerance test and insulin release test were performed. Hepatic 5α-R1 mRNA were assessed . Results: In the FSC group, body weight, abdominal circumference, blood pressure, visceral fat, plasma glucose and insulin lever significantly increased than the NC group, plasma lipid disorders increased and significant insulin resistance occurred, hepatic 5α-R1 mRNA were significantly increased , serum cortisol,corticotropin-releasing hormone level and adrenal weight had the significant relationship with the5α-R1 mRNA expression . Conclusions: We successfully induced metabolic syndrome in juvenile rat by giving high fat-salt diet .High liver 5α-R1 expression may increase GC metabolic disorder and be related to the occurrence and development of children metabolic syndrome. Targeted regulation prompted 5α-R1 expression and activity may be a early intervention for children metabolic syndrome.

Key words: high fat-salt diet, metabolic syndrome, juvenile rat, 5α-reductase type 1, adrenal cortex function