天津医药

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丙泊酚对胎儿型-和a7-烟碱型乙酰胆碱受体的作用

赵雪莲1,庄心良2,安广权2,高雁2,徐国辉2,邢玉英3   

  1. 1. 河北医科大学第四医院麻醉科
    2.
    3. 河北医科大学第四医院
  • 收稿日期:2009-11-09 修回日期:2010-01-09 出版日期:2010-07-15 发布日期:2010-07-15
  • 通讯作者: 邢玉英

Effects of propofol on embryonic- and α7- nicotinic acetylcholine receptors

  • Received:2009-11-09 Revised:2010-01-09 Published:2010-07-15 Online:2010-07-15

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摘要： 目的 通过重组受体，观察丙泊酚对胎儿型乙酰胆碱受体和神经型α7乙酰胆碱受体的作用。方法 通过脂质体转染法在HEK293细胞表达胎儿型和α7乙酰胆碱受体。分别研究不同浓度丙泊酚对两种受体亚型的作用。结果 临床相关浓度丙泊酚对100μmol/l乙酰胆碱激发胎儿型和α7乙酰胆碱受体的电流均有抑制作用。不同浓度丙泊酚对胎儿型和α7乙酰胆碱受体电流抑制大小无统计学差异（P>0.05）。相同浓度丙泊酚对胎儿型乙酰胆碱受体抑制大于对α7乙酰胆碱受体的抑制（P<0.05）。结论 从乙酰胆碱受体水平研丙泊酚的作用，结果证明丙泊酚对γ-nAChR和a7-nAChR都有抑制作用，抑制程度与丙泊酚的浓度无关。

关键词: 胎儿型烟碱型乙酰胆碱受体, a7乙酰胆碱受体, 丙泊酚

Abstract: Objective: This study was designed to examine the effects of propofol on embryonic- and α7- nicotinic acetylcholine receptors with whole cell clamp patch. Methods: The embryonic- and α7-nicotinic acetylcholine receptors were heterologously expressed in HEK293 cells and activated to maximal currents by the application of acetylcholine (100μmol/l). Peak currents were recorded in HEK293 cells treated by propofol using whole cell patch clamp technique. Results: Propofol produced rapid and readily reversible, concentration-dependent inhibition. The potence of propofol is greater to embryonic-nicotinic acetylcholine receptor compared to α7-nicotinic acetylcholien receptor(P<0.05), Propofol wasn’t result in dose-dependent inhibition of the embryonic- and α7- nicotinic acetylcholine receptors(P>0.05). Conclusion: the interactions of combination of muscle relaxants with dissimilar structure, rocuronium and atracurium, were synergistic to both adult- and embryonic- nicotinic acetylcholine receptors and with similar structure, rocuronium and vecuronium, were synergistic to adult- nicotinic acetylcholine receptors and additive to embryonic- nicotinic acetylcholine receptors.

Key words: embryonic- nicotinic acetylcholine receptor, alpha-acetylcholine receptor, propofol