天津医药

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rTMS对颞叶癫痫大鼠海马c-FOS和NGF表达的影响

宋毅军1,田小冰1,李鑫1,张雅西1,田心2   

  1. 1. 天津医科大学总医院
    2. 天津医科大学
  • 收稿日期:2010-06-18 修回日期:2010-08-05 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 田心

Effects of repetitive transcranial magnetic stimulation on the expression differences of c-FOS and NGF in the hippocampus of temporal lobe epilepsy rats

  • Received:2010-06-18 Revised:2010-08-05 Published:2011-01-15 Online:2011-01-15

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摘要： 目的：研究低频重复经颅磁刺激（rTMS）对颞叶癫痫大鼠海马结构c-FOS和NGF表达的影响。方法：Wistar大鼠共20只，随机选取5只为对照组。制备成功氯化锂匹罗卡品化学诱导的TLE模型大鼠10只，随机分为癫痫（TLE）组和重复经颅磁刺激（rTMS）组，每组5只。rTMS组给予0.5 Hz，0.4 T场强，刺激时程0.2 ms，每天20次的1周磁刺激，TLE组和对照组则给予同样次数的声音刺激(“假性”刺激)，利用免疫组织化学方法观察对照组、TLE组和rTMS组海马中c-FOS和NGF的表达情况。结果：TLE组大鼠海马区c-FOS表达明显高于对照组（P<0.01），rTMS组大鼠海马区c-FOS表达较TLE组明显降低（P<0.01），但仍高于对照组（P<0.01）。TLE组大鼠海马区NGF表达低于对照组（P<0.01），rTMS组大鼠海马区NGF表达较TLE组增高（P<0.01）。结论：适量的rTMS可降低颞叶癫痫大鼠海马中c-FOS表达，提高NGF表达，对癫痫造成的病理损伤具有神经保护作用。

关键词: 电磁场, 诱发电位, 运动, 癫痫, 颞叶, 海马, 原癌基因蛋白质c-fos, 神经生长因子类

Abstract: Objective To study the effects of low-frequency repetitive transcranial magnetic stimulation (rTMS) on the expression of NGF and c-FOS in the hippocampus of temporal lobe epilepsy (TLE) rats. Methods Lithium-pilocarpine induced TLE rat models were randomly divided into TLE group and rTMS group. Control group consist of normal rats which were littermate with the other two groups. TMS was performed at 0.5 Hz, 0.4T for 0.2ms and 20 times per day for one week on rTMS group rats. TLE group and control group rats were treated with sonic stimulation as pseudo stimuli for the same times as rTMS group. With immunohistochemical technique, we investigated the differences expression of c-Fos and NGF in the hippocampus among the three groups. Results c-Fos and NGF was positively expressed in the hippocampus of all groups. c-Fos expression level of TLE group was higher than cotrol group (p<0.01). With appropriate rTMS treatment, the expression of c-Fos of TLE rats was significantly decreased (P <0.01) but was still significantly higher than control group (p<0.01). The expression of NGF of TLE group was significantly lower than control group (P <0.01). With appropriate rTMS treatment, NGF expression of TLE group was significantly increased (P <0.01). Conclusion Appropriate rTMS can reduce the c-FOS expression while increase the NGF expression in the hippocampus of TLE rats, which plays a neuroprotective role in improving the pathological damage of epilepsy.

Key words: Electromagnetic Fields, Evoked Potentials, Motor, Epilepsy, Temporal Lobe, Hippocampus, Proto-Oncogene Proteins c-fos, 神经生长因子类