天津医药

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一个新的TSHβ亚单位剪接变体在Balb/c鼠骨髓等组织中的表达

刘浩   

  1. 天津医科大学内分泌研究所
  • 收稿日期:2010-11-18 修回日期:2010-12-15 出版日期:2011-03-15 发布日期:2011-03-15
  • 通讯作者: 刘浩

A novel thyroid stimulating hormone β-subunit splice variant in bone marrow and other tissues in Balb/c mice

  • Received:2010-11-18 Revised:2010-12-15 Published:2011-03-15 Online:2011-03-15

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摘要： 【摘要】 目的：检测Balb/c鼠的垂体、甲状腺、骨髓、脾、外周血和淋巴结中TSHβ剪接变体的表达情况。最新研究发现在人和小鼠表达一种TSHβ剪接变体，它是首次被发现含有部分内含子的TSHβ剪接体，并体外实验初步证实小鼠的TSHβ剪接变体具有生物学活性。但是，目前为止，对于它的表达部位、生理及病理学意义还不清楚。方法：本实验分别提取Balb/c鼠的垂体、甲状腺、骨髓(bone marrow, BM)、脾、外周血(peripheral blood leukocytes, PBL)和淋巴结总RNA，用DNA 酶处理后，应用RT-PCR方法检测TSHβ剪接变体的表达情况。结果：Balb/c鼠的垂体、甲状腺、骨髓、脾、外周血和淋巴结中均表达TSHβ剪接变体，其中以垂体和外周血中表达量较高。经测序证实它含有部分内含子4和整个外显子5的编码区但是没有第4外显子。结论：本实验通过确定TSHβ剪接变体在Balb/c鼠的不同组织中的表达情况，为进一步研究其生理及病理学意义奠定了基础。

关键词: 促甲状腺激素, TSHβ剪接变体, 自身免疫性甲状腺疾病

Abstract: 【Abstract】 Objective:To determine whether the novel TSHβ splice variant was expressed in pituitary, thyroid tissues，bone marrow(BM）, spleen , peripheral blood leukocytes (PBL) and Lymph nodes. It is reported that thyroid stimulating hormone β (TSHβ) splice variant is expressed in human and mouse, which is the first splice variant of TSHβ including portions of the intron. Its biology activity has been identified by mean of the assay for cAMP activity in FRTL-5 cells. However, the expression sites and the pathophysiological significance of the TSHβ splice variant remain unclear. Methods :The total RNA were isolated respectively in pituitary, thyroid tissues,BM, spleen, peripheral blood leukocytes (PBL) and Lymph nodes of Balb/c mice. The samples were treated with an RNase-Free DNase. RT-PCR was performed to detect whether TSHβ splice variant express in that tissues. Results :The TSHβ splice variant was expressed in pituitary, thyroid tissues, BM, spleen, PBL and Lymph nodes in Balb/c mice. The lever of the TSHβ splice variant expression is higher in pituitary and PBL. The sequence analysis confirms that it consists of a portion of intron 4 and all of the coding region of exon 5, but none of exon 4 codons. Conclusion: This experiment lays the foundation for the further investigation on its biological significanc through determine the expression of the TSHβ splice variant in the different tissues in Balb/c mice.

Key words: Thyroid stimulating hormone, TSHβ splice variant, Autoimmune thyroiditis