天津医药

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天津地区志贺菌耐药性及整合子携带调查

王俊1,郭文学2,祁伟3,马艳红4,吴瑾4,王淑香4   

  1. 1. 天津医科大学第二医院感染性疾病研究所
    2. 天津医科大学第二医院 天津市感染性疾病研究所
    3. 天津医大二院感染性疾病研究所
    4. 天津医科大学第二医院
  • 收稿日期:2010-06-01 修回日期:2010-08-10 出版日期:2011-05-15 发布日期:2011-05-15
  • 通讯作者: 祁伟

Changes in Patterns of Antimicrobial Susceptibility and Integron Carriage among Shigella Isolates from Tianjin

  • Received:2010-06-01 Revised:2010-08-10 Published:2011-05-15 Online:2011-05-15

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摘要： 目的：了解本地区志贺菌抗生素耐药性变迁及1、2类整合子携带状况。方法：K-B纸片法测定1981-1983年及2009年本地区临床分离的57株志贺菌药敏情况；PCR方法扩增1、2类整合子整合酶及可变区并测序分析。结果：1981-1983年组1类整合子阳性率为87.88%(29/33)，27株可变区含氨基糖苷类药物耐药基因aadA；1株宋内志贺菌可变区含甲氧苄啶耐药基因dfrA17和氨基糖苷类药物耐药耐药基因aadA5；未发现2类整合子阳性株;2009年组1类整合酶阳性率为79.17%（19/24），可变区及3'末端扩增均阴性;2类整合子阳性率87.50%（21/24），可变区含dfrA1+sat1+aadA1，介导甲氧苄啶、链丝菌素和链霉素耐药;其中17株1、2类整合酶均阳性。结论：2009年分离的志贺菌抗生素耐药较上世纪80年代分离的志贺菌增强，多重耐药菌株增加。1、2类整合子广泛存在于志贺菌中。

关键词: 整合子, 志贺菌, 耐药

Abstract: Objectives: To Investigate the change in Antibiotic resistance and carriage of class 1 and class 2 integrons of shigella isolates in Tianjin,China.Methods: A total of 57 clinical isolates of shigella identified in Tianjin during the years 1981–1983 and 2009 were studied.Antibiotic susceptibility were detected by K-B disk diffusion method .Carriage of class 1 and class 2 integrons was investigated by polymerase chain reaction (PCR) with specific primers and confirmed by restriction endonuclease analysis of amplicons.Results:In the group duing the years 1981–1983 ,Class 1 integrons were found in 87.88%(29/33) of Shigella isolates.27 of these integron 1-positive isolates contained gene cassette aadA which confer resistance to streptomycin.Only the Shigella sonnei isolates contained gene cassette dfrA17+aadA5 which confer resistance to trimethoprim and streptomycin.None integron 2-positive isolates were found;In the other group duing the years 2009,Class 1 integrase were found in 79.17%(19/24) of Shigella isolates,however,variable region and 3'conserved segment of class 1 integron were negative in all isolates. Class 2 integrons were present in 87.50%（21/24）of the Shigella isolates. All of these integron 2-positive isolates contained constant gene cassette arrays of dfrA1+sat1+aadA1 which confer resistance to trimethoprim,streptothricin and streptomycin.Both class 1 and class 2 integrase were present in 17 shigella isolates.Conclusions: Antibiotic resistance have been developed in the evolution of Shigella strains;Integron carriage were very common among the shigella isolates.

Key words: Integrons, shigella, drug-resistance