天津医药

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腺性膀胱炎IL-6、IL-17、TGF-β的表达及意义

刘敏1,2,韩瑞发1,雷铭德1,杨宇明3,谢林国2   

  1. 1. 天津市泌尿外科研究所
    2. 天津医科大学第二医院
    3. 天津医科大学第二医院泌尿外科天津市泌尿外科研究所
  • 收稿日期:2011-09-07 修回日期:2012-01-12 出版日期:2012-06-15 发布日期:2012-06-15
  • 通讯作者: 韩瑞发

Expression of IL-6, IL-17, TGF-β in Cystitis Glandularis and their significances

  • Received:2011-09-07 Revised:2012-01-12 Published:2012-06-15 Online:2012-06-15

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摘要： 摘要 目的：研究IL-6、IL-17和TGF-β在腺性膀胱炎(CG)组织的表达，探讨非可控炎症在CG发病及进展中的作用。 方法：将15例正常膀胱黏膜定为对照（A组），43例未复发CG定为治愈（B组），13例复发和癌变的CG定为进展（C组）；采用免疫组化SABC法测定三组中IL-6、IL-17和TGF-β的表达情况，χ2检验分析三组阳性表达率有无差异；Spearman相关性分析上述因子对CG术后进展情况的相关性。P<0.05为差别具有统计学意义。 结果：各组中IL-6、IL-17和TGF-β的阳性表达率分别为A组：20%（3/15）、6.7%（1/15）、33.3%（5/15）；B组：46.5%（20/43），41.9%（18/43），39.5%（17/43）；C组：76.9%（10/13），76.9%（10/13），69.3%（9/13）。表达率差异有统计学意义(χ2=15.824、23.036、14.274；P=0.003、0.000、0.006);各组年龄与性别差异无统计学意义(P >0.05)。3种因子与CG进展具有相关性（r =0.728、0.785、0.659；P=0.000、0.000、0.000）；IL-17分别与IL-6、TGF-β成明显正相关（r=0.997、0.761；P=0.000、0.000）。结论：IL-6、IL-17和TGF-β的持续过表达及三者之间的相互作用可能在CG发病及进展中起重要作用，更进一步提示非可控炎症可能在CG的发病及进展中起重要作用。

关键词: 腺性膀胱炎, 白细胞介素类, β型转化生长因子, 非可控炎症, 免疫组织化学

Abstract: Abstract Objective: To study the expression of IL-6, IL-17 and TGF-β in the tissue of cystitis glandularis(CG), investigate the role of nonresolving inflammation in the pathogenesis and development of CG. Methods: Collect 15 specimens of normal mucous membrane of bladder as control group (Group A)，43 specimens of curative cystitis glandularis as (Group B); 13 specimens of recurrent and cancerate cystitis glandularis as progression (Group C); Immunohistochemical (SABC) method was used to detect the expression of interleukin-6(IL-6), interleukin-17(IL-17) and TGF-β in the 3 groups. χ2 test was used to analyze the positive rates of the 3 factors in the 3 groups; Spearman rank correlation test was used to analyze the dependability of the 3 factors. Results: Positive rates of IL-6, IL-17, TGF-β in Group A、B、C: Group A were 20%（3/15）、13.3%（2/15）、33.3%（5/15）; Group B were 60.5%（26/43），62.8%（27/43），51.2%（22/43）; Group C were 92.3%（12/13），100%（13/13），100%（13/13）.There were significant differences between the 3 groups (χ2=15.824、23.036、14.274；P=0.003、0.000、0.006); there were no significant differences in expression levels of 3 groups between the different age and gender(P >0.05). Positive correlation exited between IL-6, IL-17,TGF-β and progression（r =0.728、0.785、0.659；P=0.000、0.000、0.000）；positive correlation existed between IL-17 and IL-6,TGF-β（r=0.997、0.761；P=0.000、0.000）. Conclusion: Positive expression of IL-6, IL-17, TGF-β and the synergetic effect of the 3 factors may play important roles in the pathogenesis and development of CG, further more nonresolving inflammation may play an important role in the pathogenesis and development of CG.

Key words: cystitis glandularis interleukins transforming growth factor, β type nonresolving inflammation immunohistochemistry