天津医药

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黄芩苷对结核分枝杆菌抑菌作用的初步研究

邵世峰1,孙婉蓉2,张丽霞1,肖红侠1,张妍1,张立1,吴琦1   

  1. 1. 天津市海河医院
    2. 天津医科大学总医院
  • 收稿日期:2012-01-17 修回日期:2012-04-24 出版日期:2012-08-15 发布日期:2012-08-15
  • 通讯作者: 邵世峰

Study of antituberculosis action of baicalin on Mycobacterium tuberculosis

  • Received:2012-01-17 Revised:2012-04-24 Published:2012-08-15 Online:2012-08-15

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摘要： 目的：通过体外抑菌试验探讨黄芩苷对结核分枝杆菌的体外抑菌活性。方法：采用改良罗氏培养基绝对浓度法检测黄芩苷对结核分枝杆菌的抑菌作用。结果：黄芩苷对37株结核分枝杆菌临床分离株的最低抑菌浓度(MIC)最低为1.5 g/L，最高＞48 g/L，MIC90为12g /L， MIC75和MIC50均为6g /L。全敏感菌株在MIC为6 g/L时数量比例最大，随着MIC值向两侧递减；异烟肼(INH)低耐菌株的数量与MIC值无明显关联；乙胺丁醇(EMB)低耐菌株数量随着MIC（3-9g/L范围内）由低到高的变化而显著增多；利福平(RFP)低耐菌株数量随着MIC（2-8g/L范围内）由低到高的变化而逐步减少。结论：黄芩苷在体外对结核分枝杆菌具有一定的抑菌作用。

关键词: 黄芩苷, 结核分枝杆菌, 抑菌作用

Abstract: object: To analyze the antituberculosis action of baicalin on Mycobacterium tuberculosis by Antimicrobial Susceptibility Testing in vitro. methods: Detecting Bacteriostatic Action of baicalin on Mycobacterium tuberculosis by absolute consistence method of Lowenstein-Jensen. results: MIC minimum of baicalin on 37 strains Mycobacterium tuberculosis was 1.5 g/L, MIC maximum was ＞48 g/L.MIC90 was 12 g/L, MIC75 and MIC50 was 6g/L.The number of drug sensitive Mycobacterium tuberculosis was most in 6 g/L MIC, and decrease progressively with MIC value reduce or increase. there was not relevance between the strain number of INH mild drug resistance Mycobacterium tuberculosis and the MIC value. The number of EMB mild resistance strain was increase progressively with MIC value increase（MIC 3-9g /L）. The number of RFP mild resistance strain was decrease progressively with MIC reduce（MIC 2-8 g/L）.conclusion: there is antituberculosis action of baicalin on Mycobacterium tuberculosis.

Key words: baicalin, Mycobacterium tuberculosis, Bacteriostatic Action