天津医药

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凋膜止崩液对ADUB模型大鼠子宫内膜组织ER及α、β亚型表达的影响

贺丰杰,李小宁   

  1. 陕西中医学院
  • 收稿日期:2011-12-20 修回日期:2012-03-17 出版日期:2012-08-15 发布日期:2012-08-15
  • 通讯作者: 贺丰杰

The Effects of Diaomozhibeng liquid Uterine Cavity Target Intervention on Uteri Endometrial tissue of ADUB in Model Rats on the Expression of ER、 ERα、ERβ

Feng-Jie HE 2   

  • Received:2011-12-20 Revised:2012-03-17 Published:2012-08-15 Online:2012-08-15
  • Contact: Feng-Jie HE

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摘要： 目的 探讨凋膜止崩液宫腔靶向给药治疗无排卵功血(ADUB)子宫内膜增生症的可能机制，为临床治疗ADUB提供一种新方法。方法 卵巢去势后利用雌激素负荷法建立ADUB子宫内膜增生症大鼠模型，凋膜止崩液宫腔内给药后处死，留取子宫标本，采用免疫组化技术检测各组大鼠子宫内膜组织中雌激素受体（estrogen receptor,ER）、ERα、β亚型的表达变化。结果 模型对照组与空白组比较，ER、ERα、β表达明显升高，差异有统计学意义（P＜0.05）。实验大、小剂量组与模型对照组比较，ER、ERα、β表达下调，差异有统计学意义（P＜0.05），而实验大、小剂量组间比较无显著性差异（P＞0.05）。结论 凋膜止崩液使ADUB模型大鼠子宫内膜组织ER、ERα、β表达下调，阻断了雌激素发挥效应的通路，从而抑制子宫内膜的持续性增生，达到了治疗子宫内膜增生症的目的。

关键词: 凋膜止崩液, ADUB, 靶向治疗, 雌激素受体, 雌激素受体亚型

Abstract: Objective: To explore the role and the possible mechanism of Diaomo Zhibeng liquid on the rats with the endometrial hyperplasia. Methods: The model of endometrial hyperplasia was induced with estrogen load after the induction of model in rats with EH. The medicine of Diaomo Zhibeng liquid were administrated in uterine cavity of rats.The expression levels of ER、ERα、β were checked by immunohistochemistry. Results: Compared with control group, the expression of ER、ERα、β increased in the rats of the model group (P<0.05). However, after treatment of Diaomo Zhibeng liquid, the expression of ER、ERα、β decreased, (P<0.05). There is no difference between small and big dose of Diaomo zhibeng liquid groups (P>0.05). Conclusion: Diaomo Zhibeng liquid plays a role in the endometrial hyperplasia by accelerate the ecclasis of uterine endometrial. One of mechanisms is maybe related to inhibiting the expression of ER、ERα、β.

Key words: Diaomozhibeng liquid, ADUB, Target treatment, ER, ERα、β