天津医药

• 论著 • 上一篇    下一篇

Musashi-2、CD133在结肠腺癌中的表达

王娅菲,林中   

  1. 桂林医学院附属医院消化内科
  • 收稿日期:2013-01-11 修回日期:2013-07-30 出版日期:2013-12-15 发布日期:2013-12-15
  • 通讯作者: 王娅菲

The Expressions of Musashi-2and CD133Protein in Colonic Adenocarcinoma

WANG Ya fei,LIN Zhong   

  1. Affiliated Hospital of Guilin Medical University
  • Received:2013-01-11 Revised:2013-07-30 Published:2013-12-15 Online:2013-12-15
  • Contact: WANG Ya fei

PDF (PC)

2712

摘要：

【摘要】  目的  研究Musashi-2、CD133在结肠腺癌组织和正常结肠黏膜组织中的表达及其与结肠腺癌发生发展的关系。  方法  采用免疫组织化学法检测并比较40例结肠癌组织（结肠腺癌组）及15例正常结肠黏膜组织（对照组）中Musashi-2和CD133蛋白的表达，分析结肠腺癌组Musashi-2、CD133蛋白的表达与临床病理参数的关系。  结果   结肠腺癌组Musashi-2、CD133蛋白的阳性表达率分别是60%、27.5%，对照组分别是26.7%和0，结肠腺癌组均高于对照组（χ2分别为4.850、5.156，均P＜0.05）。组织分化程度越低、TNM分期越高，Musahi-2蛋白的阳性表达率越高；组织分化程度越低、出现淋巴结转移，CD133蛋白的阳性表达率越高（均P＜0.05）。  结论  Musashi-2和CD133可能跟结肠腺癌的发生发展有关，可将其做为结肠腺癌干细胞标志物进一步研究。

关键词: 结肠肿瘤, 腺癌, 免疫组织化学, Musashi-2, CD133

Abstract:

[Abstract]   Objective   To investigate the expression of Musashi-2and CD133in colonic adenocarcinoma，and their
correlation with the occurrence and development of colonic adenocarcinoma thereof.  Methods   The expressions of Musashi-2and CD133protein were detected by immunohistochemical method in40colonic adenocarcinoma samples and15normal colonic structure samples. The different histological types, TNM staging, lymph node metastasis, serous infiltration, distant metastasis and expressions of Musashi-2and CD133proteins in colonic adenocarcinoma tissues were analyzed.  Results   The positive expression rates of Musashi-2and CD133protein were60% and27.5% in40colonic adenocarcinoma samples and26.7% and0in15normal colonic samples, which showed the significantly higher expression rates in colonic adenocarcinoma group than those of control group (χ2=4.850and5.156,P<0.05). There were significant differences in expressions of Musashi-2proteins between different histological stages and TNM staging (P<0.05). The positive expression rate of Musahi-2protein increased as the degree of differentiation decreased and TNM stage increased. There were significant differences in expressions of CD133proteins between different histological stages and lymph node metastasis (P<0.05). The positive expression rate of CD133protein increased as the degree of differentiation decreased and lymph node metastasis occurred.    Conclusion   The expression of Musashi-2and CD133may be related with the initiation and development of colonic cancer, which can be used as the stem cell markers of colonic adenocarcinoma for the further study.

Key words: colonic neoplasms, adenocarcinoma, immunohistochemistry, Musashi-2, CD133