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应用组织芯片探讨hCTR1在子宫颈鳞癌、CIN中的表达及意义

刘洪博   

  1. 河北省衡水市哈励逊国际和平医院 病理科
  • 收稿日期:2012-04-24 修回日期:2012-08-24 出版日期:2013-01-15 发布日期:2013-01-15
  • 通讯作者: 刘洪博

Detecting the expression and significance of hCTR1 in cervical carcinoma and CIN by using tissue microarray

  • Received:2012-04-24 Revised:2012-08-24 Published:2013-01-15 Online:2013-01-15

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摘要：

【摘要】 目的探讨人类铜转运蛋白(hCTR1)在子宫颈鳞癌、宫颈上皮内瘤样病变(CIN)中的表达及其临床病理意义。方法利用组织芯片技术和免疫组化染色方法,检测正常宫颈组织23例、CIN组织94例及子宫颈鳞癌组织52例中hCTR1蛋白的表达。结果 (1)自制组织芯片切片免疫组化染色成功。(2)子宫颈鳞癌组(69.2%)、CINⅡ～Ⅲ组(73.1%)的hCTR1阳性率均高于正常宫颈组(17.4%)及CINⅠ组(29.6%),差异有统计学意义(P<0.001)。(3)hCTR1表达在不同年龄、临床分期、肿瘤大小、病理分级及有无淋巴结转移子宫颈鳞癌患者之间差异均无统计学意义(P>0.05)。结论应用组织芯片技术减少了实验误差,hCTR1蛋白的阳性表达率可辅助宫颈病变诊断,术后检测hCTR1蛋白对指导子宫颈癌患者的治疗有一定的帮助。 

关键词: 人类铜转运蛋白, 子宫颈鳞癌, 顺铂, 组织芯片技术

Abstract: Abstract:Purpose To investigate the expression and clinical pathological significance of hCTR1 in the cervical carcinoma and cervical intraepithelial neoplasia. Methods Using tissue microarray and immunohistochemical staining to detect the expression of hCTR1 in 52 cases of cervical carcinoma, 94 cases of CIN and 23 cases of normal cervical squamous epithelia. Results ①The manual tissue microarray was satisfied in IHC staining,and it satisfied the requirement of our studying multiple samples,the detetectable rate was 89.4%.②The positive retas of hCTR1 in SCC(69.2%) and in the level CIN(CIN 2-3, 73.1%) were significantly higher than those of normal cervical squamous epithelium(17.4%) and CIN 1(29.6%) (P<0.05). Other groups are no significant different(P>0.05). ③No significant relationships were found between the expression of hCTR1 and clinical pathological features. Conclusions Using manual tissue microarray made all the cases under the same experiment standard, could minimize the experimental error. hCTR1 expression rates became higher and higher from normal cervical squamous epithelia, CIN to cervical squamous cell carcinoma.hCTR1 regulates the cisplatin concentration in tumor cells so that it may be used as a potential biomarker of cervical carcinoma.

Key words: hCTR1, cervical squamous cell carcinoma, cisplatin, tissue microarray techniques