大鼠缺血性急性肾损伤对海马CA1区的形态学影响

大鼠缺血性急性肾损伤对海马CA1区的形态学影响

朱立志¹,李郭锦¹,田鹤²,郭敏²

1. 辽宁医学院附属第三医院
2. 辽宁医学院组织胚胎学教研室

收稿日期:2013-08-27 修回日期:2013-10-11 出版日期:2014-02-15 发布日期:2014-02-15
通讯作者: 郭敏

Morphological effects of ischemic acute kidney injury on neurons of hippocampus CA1 in rats

Received:2013-08-27 Revised:2013-10-11 Published:2014-02-15 Online:2014-02-15

朱立志1 ,李郭锦1 ,田鹤2 ,郭敏2 . 大鼠缺血性急性肾损伤对海马CA1区的形态学影响[J]. .

摘要/Abstract

摘要： 目的观察大鼠急性缺血性肾损伤对海马CA1区神经元的形态学影响。方法成功制造缺血性急性肾损伤大鼠模型后应用光镜电镜技术、免疫组织化学及免疫印迹技术对大鼠缺血性急性肾损伤的海马CA1区进行了观察和分析。结果肾缺血60 min再灌注24 h后光镜下海马CA1区锥体细胞层出现了固缩性神经元，经电子显微镜观察发现细胞萎缩，基质电子密度增高，细胞核萎缩但无染色质固缩表现，内质网扩张、线粒体肿胀变性。免疫组化染色及免疫印迹结果揭示急性肾损伤的海马caspase-3阴性表达而 PARP-1表达明显增强。结论大鼠缺血性急性肾损伤可引起海马CA1区锥体细胞层神经元固缩性损伤，既细胞质性死亡，推测这种损伤可能属于PARP-1介导的细胞死亡。

关键词: 缺血性急性肾损伤, 海马神经元, 固缩性损伤, PARP-1介导的细胞损伤

Abstract: Objective To observe neuron changes of hippocampus CA1 induced by ischemic acute kidney injury (IAKI) in rats. Methods Based on successful established IAKI animal models light and electron microscope, immunohistochemical (IHC)staining and Western blot were used for the present observations. Results Pyknotic neurons in CA1 of hippocampus were found after renal 60 min ischemia and 24 h reperfusion. The examinations of electron microscope provided facts that swollen mitochondria and dilated endoplasmic reticule in cytoplasm and shrinkage nucleus with no pyknotic chromatin were found in the pyknotic neurons. IHC staining observations revealed that the pyknotic neuron showed negative caspase-3 staining but positive PARP-1 staining. In addition, immunoblot analysis provided that only PARP-1 expressed much stronger in IAKI associated hippocampus. Conclusion The pyknotic neurons induced by IAKI might be of PARP-1 mediated cell death and of cytoplasmic death morphologically.