天津医药

• 细胞与分子生物学 • 上一篇    下一篇

香加皮杠柳苷对人肺癌QG56细胞抑制作用的研究

张静1,杨光1,赵学涛1,单保恩2,刘江惠1   

  1. 1. 河北医科大学第四医院
    2. 河北医科大学第四医院 河北省肿瘤研究所
  • 收稿日期:2013-07-02 修回日期:2013-10-28 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 张静

Inhibitory Effects of Periplocin from Cortex Periplocae in Human Lung Cancer Cell Line QG56

  • Received:2013-07-02 Revised:2013-10-28 Published:2014-03-15 Online:2014-03-15
  • Contact: Jing ZHANG

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摘要： 　　　[摘要] 目的：研究香加皮杠柳苷（CPP）对人肺癌QG56细胞的抑制作用及其作用机理。方法：体外培养人肺癌QG56细胞，采用MTT法检测不同浓度CPP（1.25、2.50、5.00、10.00、20.00 ng/ml）处理24 h、48 h、72 h对人肺癌QG56细胞的增殖抑制作用；应用倒置显微镜观察CPP处理前后细胞形态变化；应用流式细胞术分析CPP对细胞周期和细胞凋亡的影响；采用RT-PCR法检测CPP作用后QG56细胞中凋亡相关基因bax的mRNA表达情况；应用免疫细胞化学法检测CPP对QG56细胞bax蛋白表达的影响。结果：MTT结果显示，CPP能显著抑制人肺癌QG56细胞的增殖。显微镜下可见CPP处理后的QG56细胞变圆，皱缩，呈悬浮状态。流式细胞术检测结果显示，与对照组相比，CPP组G0/G1期细胞显著增多，而S期和G2/M期细胞显著减少，且QG56细胞的凋亡率明显升高(P<0.05)。RT-PCR结果显示，CPP处理后QG56细胞中bax mRNA表达增加。免疫细胞化学结果显示，CPP组细胞中bax蛋白表达明显增强。结论：香加皮杠柳苷（CPP）具有明显的抑制人肺癌QG56细胞增殖作用，其机制可能与阻滞细胞周期和诱导细胞凋亡有关。

关键词: 香加皮杠柳苷, 肺癌, 细胞周期, 凋亡

Abstract: 　　　[ABSTRACT] Objective: To investigate the inhibitory effects of Periplocin from Cortex Periplocae (CPP) in human lung cancer cell line QG56 and to discuss its mechanism. Methods: Inhibitory effect of CPP in different concentrations (1.25, 2.50, 5.00, 10.00, 20.00 ng/ml) and different time (24 h, 48 h, 72 h) on QG56 proliferation was tested by MTT method. The morphological changes of the QG56 cells treated with CPP were observed by inverted microscope. The effects of CPP on cell cycle and cell apoptosis were determined by flow cytometry (FCM). Expression of apoptosis associated genes bax on cells was assessed by RT-PCR and SP immunocytochemistry method respectively before and after treatment of CPP. Results: Marked inhibitory effect of CPP on QG56 cells was observed. The morphological changes were displayed in QG56 exposed to CPP. The results of flow cytometry showed that the proportion of tumor cells in the G0/G1 phase was increased markedly, which of S and G2/M phase cells decreased, after treatment with CPP. The apoptosis rate of QG56 cells in CPP groups increased significantly compared to the control group (P0.05). The expression of bax mRNA was increased in QG56 exposed to CPP. The result of immunocytochemistry indicated that bax protein showed stronger positive staining in cells with CPP treatment. Conclusion: CPP has significant inhibitory effect on human lung cancer cell lines QG56, which is probably related to blocking cell cycle and induction of apoptosis.

Key words: Periplocin from Cortex Periplocae(CPP), human lung cancer, cell cycle, apoptosis