天津医药

• 细胞与分子生物学 • 上一篇    下一篇

周细胞在子宫内膜异位症血管生成中的作用

赵采云   

  1. 天津医科大学第二医院
  • 收稿日期:2013-09-03 修回日期:2013-12-22 出版日期:2014-04-15 发布日期:2014-04-15
  • 通讯作者: 赵采云

Roles of Pericyte in the Angiogenesis of Endometriosis

ZHAO cai yun   

  1. Department of Gynaecology, the Second Hospital of Tianjin Medical University, Tianjin300211, China
  • Received:2013-09-03 Revised:2013-12-22 Published:2014-04-15 Online:2014-04-15
  • Contact: ZHAO cai yun

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摘要： 摘要：目的 探讨周细胞在子宫内膜异位症（EMs）血管生成中的作用。方法 采用免疫组化方法（SP法）检测60例子宫内膜异位症患者（EMs组）在位内膜（增殖期、分泌期内膜各20例）、异位内膜20例及40例非子宫内膜异位症患者（对照组）子宫内膜（增殖期、分泌期各20例），分别选用α-SMA标记平均周细胞数（PN）和CD34标记微血管密度（MVD），并对二者比值（PN/MVD）进行比较，分析周细胞与EMs血管生成之间的关系。结果 EMs组异位内膜MVD高于正常增殖期和正常分泌期内膜（P<0.05），在位内膜无论增殖期还是分泌期的MVD与相应的正常内膜比较差异均无统计学意义，在位内膜及正常内膜组内比较均为分泌期高于增殖期（P<0.05）。EMs组异位内膜PN低于正常增殖期内膜（P<0.05），与正常分泌期内膜相比差异无统计学意义，在位内膜无论增殖期还是分泌期的PN与相应的正常内膜相比差异均无统计学意义，组内比较均分泌期低于增殖期（P<0.05）；EMs组异位内膜的PN/MVD低于正常增殖期和正常分泌期内膜（P<0.05）。在位内膜无论增殖期还是分泌期的PN/MVD与相应的正常内膜比较，均为在位内膜低于正常内膜（P<0.05），组内比较均分泌期低于增殖期（P<0.05）。结论 周细胞对子宫内膜异位症血管生成起着重要作用，周细胞覆盖率是判定子宫内膜血管成熟度和稳定性的重要指标。

关键词: 子宫内膜异位症, 血管生成, 微血管密度, 周细胞

Abstract: Abstract: Objective To study the expression of pericyte and make further discussion about the association between pericyte and angiogenesis in the endometriosis. Methods the main group consisted of 20 ectopic endometrium 、20 eutopic endometrium during proliferation phase,20 eutopic endometrium during secretory phase of patient with endometriosis .The control group presented 20 normal endometrium during proliferation phase and 20 normal endometrium during proliferation phase of women without entometriosis.The histological morphology ,mean density of microvessel(MVD),pericytes number（PN） as well as the average ratio of pericyte to endothelial cells in microvessels were measured by immunohistochemical and morphological assesses .Results The expression of MVD was increased from the ectopic endometrum tissue ,eutopic endometrum to normal endometrum,while the expression of PN and PN/MVD was opposite.we also found that the PN in eutopic endometrum was slightly lower than it in normal endometrium ,no mater in secretory stage or proliferativa stage,there was no statistical difference between them.But The difference of the PN/MVD had significant meaning (P<0.01). Conclusion Pericytes play an important role in the angiogenesis of the endometriosis ,pericyte coverage contributing to a mature functional vasculature of endometriosis.

Key words: endometriosis, angiogenesis, microvessel, pericyte