关键词: 碳酸饮料, 牙乳头干细胞, 骨保护素, 维生素D受体

Abstract: Abstract Objective:To research for the mechanism of carbonated drinks affecting health of the children primary and permanent teeth replacement. Methods:The dogs tooth enamel samples were soaked in coca-cola, sprite and pure soda, and the calcium, phosphorus concentrations were detected. Dental papilla stem cells were separated and cultured in the conditioned medium by adding three drinks. PCR and western blot method were used to detect the mRNA and protein expression levels of activator of nuclear factor-k B receptor ligand (RANKL), osteoprotegerin (OPG) and vitamin D receptor (VDR) , then the possible role of each gene and interactions relationship were analyzed. Results:Compared with saline, coca-cola and sprite showed their significantly decalcification and dephosphorization role, while plain soda water showed calcium and phosphorus protective effect. The three drinks had no effect on the mRNA and protein levels of RANKL gene (P> 0.05). Coca-cola and sprite can reduce OPG mRNA and protein levels, and at the same time increase the expression of the VDR gene. Plain soda water has no effect on the OPG gene expression, but can significantly reduce the level of the VDR gene. Conclusion:Carbonated drinks may affect the dental health of the children's primary and permanent teeth replacement by regulating bone related gene expression and vitamin D receptor family.

Key words: Carbonated drinks, Dental papilla stem cells, O P G, Vitamin D receptor