天津医药

天津医药 ›› 2022, Vol. 50 ›› Issue (7): 757-761.doi: 10.11958/20220359

• 临床研究 • 上一篇    下一篇

呼吸道合胞病毒毛细支气管炎婴幼患儿外周血miR-155与PDCD4表达的相关性研究

王刚，王琼，姜昕，郑静   

  1. 1上海浦滨儿童医院儿内科（邮编200120）；2上海曲阳医院大白安心儿科特诊部；3上海中医药大学基础医学院免疫学与病原生物学教研室；4上海中医药大学基础医学院生物化学教研室
  • 收稿日期:2022-03-09 修回日期:2022-04-08 出版日期:2022-07-15 发布日期:2022-07-15
  • 基金资助:
    上海市科研计划项目（18ZR1439700）

Correlation between miR-155 and PDCD4 expression in peripheral blood of infants with respiratory syncytial virus bronchiolitis

WANG Gang, WANG Qiong, JIANG Xin, ZHENG Jing   

  1. 1 Department of Pediatrics, Shanghai Pubin Children's Hospital, Shanghai 200120, China; 2 Dabaidoc Pediatrics, Shanghai Quyang Hospital; 3 Department of Immunology and Pathogen Biology, Shanghai University of Traditional Chinese Medicine; 4 Department of Biochemistry, Shanghai University of Traditional Chinese Medicine
  • Received:2022-03-09 Revised:2022-04-08 Published:2022-07-15 Online:2022-07-15

PDF (PC)

2109

摘要： 目的　探究呼吸道合胞病毒（RSV）毛细支气管炎婴幼儿外周血miR-155与程序性细胞死亡因子4（PDCD4）表达的关系。方法　选取106例RSV毛细支气管炎婴幼儿为研究组，根据病情严重程度分为轻症组36例、中症组41例和重症组29例。选择健康体检婴幼儿65例为对照组。采用实时荧光定量PCR（qPCR）法检测婴幼儿外周血血清miR-155水平；采用酶联免疫吸附试验（ELISA）检测PDCD4、超敏C反应蛋白（hs-CRP）、白细胞介素（IL）-6、IL-10、肿瘤坏死因子（TNF）-α水平；Pearson相关分析RSV毛细支气管炎婴幼儿血清miR-155和PDCD4的关系，以及两者与炎性因子的相关性。结果　与对照组相比，研究组患儿血清miR-155水平升高，PDCD4水平降低（P＜0.05）。且随着RSV毛细支气管炎严重程度的加重，血清miR-155水平上升，PDCD4水平下降（P＜0.05）。与对照组相比，研究组的血清炎性因子hs-CRP、IL-6、IL-10、TNF-α水平均上升（P＜0.05）。Pearson相关性分析显示，miR-155和PDCD4呈负相关（r=-0.790，P＜0.01）。且miR-155与hs-CRP、IL-6、IL-10、TNF-α均呈正相关，PDCD4与hs-CRP、IL-6、IL-10、TNF-α均呈负相关（均P＜0.01）。结论　RSV毛细支气管炎婴幼儿血清miR-155、PDCD4水平异常表达，两者呈负相关关系，且其水平与炎性因子密切相关。

关键词: 呼吸道合胞病毒, 毛细支气管炎, 病毒性, miR-155, 程序性细胞死亡因子4, 婴幼儿

Abstract: Objective　To explore the relationship between the expression of miR-155 and programmed cell death 4 (PDCD4) in peripheral blood of infants with respiratory syncytial virus (RSV) bronchiolitis. Methods　A total of 106 infants and young children with RSV bronchiolitis accepted by our hospital were collected as the research group. According to the severity of the disease, patients were separated into the mild group (n=36), the moderate group (n=41), and the severe group (n=29). Sixty-five infants who underwent physical examination in the child care clinic of our hospital were selected as the control group (n=55). The serum level of miR-155 in peripheral blood of infants and young children was measured by real-time quantitative PCR (qPCR) method. Levels of PDCD4, high sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assay (ELISA). The relationship between serum miR-155 and PDCD4 and their correlation with inflammatory factors in infants with RSV bronchiolitis were analyzed by Pearson correlation. Results　Compared with the control group, the serum level of miR-155 was significantly increased in the study group, and the level of PDCD4 was significantly decreased (P＜0.05). In addition, the serum miR-155 level increased with the aggravation of the severity of RSV bronchiolitis, and the PDCD4 level reduced significantly (P＜0.05). Compared with the control group, serum levels of inflammatory factors hs-CRP, IL-6, IL-10 and TNF-α increased significantly in the study group (P＜0.05). Pearson correlation analysis showed that miR-155 and PDCD4 were negatively correlated (r=-0.790, P＜0.01), miR-155 was positively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01), and PDCD4 was negatively correlated with hs-CRP, IL-6, IL-10, and TNF-α (P＜0.01). Conclusion　RSV bronchiolitis in infants and young children has abnormal expression of serum levels of miR-155 and PDCD4, and the two levels are negatively correlated. Serum levels of miR-155 and PDCD4 are tightly related to inflammatory factors.

Key words: respiratory syncytial viruses, bronchiolitis, viral, miR-155, programmed cell death factor 4, infants and young children