天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (3): 248-252.doi: 10.11958/20202682

• 实验研究 • 上一篇    下一篇

艾灸减轻新生小鼠缺氧缺血性脑病的作用及其机制研究

欧阳昕1，徐优慧2，陈明人3，肖爱娇2△   

  1. 1江西中医药大学研究生院（邮编330004），2中医学院，3针灸推拿学院
  • 收稿日期:2020-09-29 修回日期:2020-11-25 出版日期:2021-03-15 发布日期:2021-03-15
  • 通讯作者: 肖爱娇 E-mail:xaj527@163.com
  • 作者简介:欧阳昕（1997），男，硕士在读，主要从事中医药治疗神经系统疾病的效应及机制研究。E-mail：oyx53@163.com
  • 基金资助:
    国家自然科学基金资助项目（81660819）；江西省科技厅重点研发计划项目（20161BBH80053）；江西省教育厅科学技术研究重点项目（GJJ201208）

Study on the mechanism of moxibustion in attenuating hypoxia-ischemic brain injury in neonatal mice

OUYANG Xin1, XU You-hui2, CHEN Ming-ren3, XIAO Ai-jiao2△   

  1. 1 Graduate School, 2 School of Traditional Chinese Medicine, 3 School of Acupuncture and Moxibustion, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • Received:2020-09-29 Revised:2020-11-25 Published:2021-03-15 Online:2021-03-15

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摘要： 目的　研究艾灸减轻新生小鼠缺氧缺血性脑病（HIE）的作用及其机制。方法　55只出生7 d的ICR小鼠按照随机数字表法分为假手术组（Sham组）、模型组（HI组）和艾灸组（MOX组）。HI组和MOX组采用右侧颈总动脉结扎联合缺氧法制备HIE模型，MOX组建模后艾灸刺激大椎穴，35 min/次，1次/d，连续治疗3 d。处理结束后麻醉取脑，DAPI染色观察脑组织形态结构，TUNEL染色检测脑组织细胞凋亡；免疫荧光染色检测脑组织caspase-9表达；放射免疫分析法测定脑组织caspase-3含量。结果　与Sham组相比，HI组小鼠脑组织排列疏松，脑组织凋亡细胞数和caspase-9表达显著增多（P＜0.05）；与HI组相比，MOX组小鼠脑组织排列较致密，脑组织凋亡细胞数、caspase-9和caspase-3表达显著减少（P＜0.05）。结论　艾灸可能通过降低脑组织caspase-9和caspase-3的表达来减少细胞凋亡，从而减轻新生小鼠缺氧缺血性脑损伤。

关键词: 艾条灸；缺氧缺血, 脑；脑损伤；细胞凋亡；半胱氨酸天冬氨酸蛋白酶9；半胱氨酸天冬氨酸蛋白酶3；新生小鼠

Abstract: Objective　To investigate the mechanism of moxibustion on hypoxia-ischemic encephalopathy (HIE) in neonatal mice. Methods　A total of 55 neonatal ICR mice (aged 7 days) were randomly divided into sham-operated group (Sham group), HI group and moxibustion-treated (MOX) group according to the method of random digital table. Mice in both HI group and MOX group were induced HIE model by ligation of the right common carotid artery combined with hypoxia in a closed transparent chamber. After the model was established, mice in MOX group were given moxibustion treatment by heating Dazhui points 35 min/d, once a day for 3 days. The morphological structure of brain tissue was examined by DAPI staining. The apoptotic cells of brain tissue were detected using TUNEL assay. The expression of caspase-9 was detected by immunofluorescent staining, and the content of caspase-3 was determined by radioimmunoassay. Results　Compared with the Sham group, mice in the HI group showed that the arrangement of brain tissues was loose, and the number of apoptotic cells, the expression of caspase-9 positive cells in brain tissues increased (P＜0.05). Compared with the HI group, mice in the MOX group exhibited that the arrangement of brain tissues was relatively compact, and the number of apoptotic cells, the expression of caspase-9 and the content of caspase-3 in brain tissues decreased significantly (P＜0.05). Conclusion　Moxibustion may decrease caspase-9 expression and caspase-3 content of brain tissues and then reduce apoptosis, thus reducing hypoxic-ischemic brain injury in neonatal mice.

Key words: Moxa Stick Moxibustion, hypoxia-ischemia, brain, brain injuries, apoptosis, caspase-9, caspase-3, neonatal mouse