天津医药

天津医药 ›› 2021, Vol. 49 ›› Issue (7): 769-.doi: 10.11958/20203656

• 诊断技术 • 上一篇    下一篇

尿液microRNA-21表达联合三维超声成像技术对膀胱尿路上皮癌的诊断价值

王琳，宣之东，张颖，刘永容，李超，姜利宁   

  1. 1河北省沧州市中心医院超声三科（邮编061000），2泌尿外科
  • 出版日期:2021-07-15 发布日期:2021-07-12
  • 作者简介:王琳（1987），女，硕士，主治医师，主要从事泌尿系肿瘤超声诊断研究。E-mail：337890823@qq.com

The diagnostic value of urine microRNA-21 expression combined with 3D-US for bladder urothelial carcinoma

WANG Lin, XUAN Zhi-dong, ZHANG Ying, LIU Yong-rong, LI Chao, JIANG Li-ning   

  1. 1 The Third Department of Ultrasonography, 2 Department of Urology, Cangzhou Central Hospital, Cangzhou 061000, China
  • Published:2021-07-15 Online:2021-07-12

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摘要： 目的　探讨尿液中microRNA-21（miR-21）表达联合三维超声成像技术（3D-US）在膀胱尿路上皮癌（BUC）诊断中的临床应用价值。方法　收集78例BUC患者（病例组）和80例良性泌尿系疾病患者（对照组）的尿液，应用实时荧光定量PCR（qPCR）检测尿液中miR-21的表达水平；分析miR-21水平与临床病理学特征的关系；行3D-US检查，并采用受试者工作特征（ROC）曲线分析尿液miR-21及miR-21联合3D-US对BUC诊断的临床价值。结果　病例组患者尿液miR-21的相对表达量显著高于对照组（P＜0.01）。尿液miR-21在浸润组中含量明显高于非浸润组，在高级别组中表达明显高于低级别组（P＜0.05）；ROC曲线分析显示：尿液miR-21表达联合3D-US诊断BUC的敏感度为94.87%，特异度为96.25%，约登指数接近于膀胱镜检查。结论　BUC患者尿液中miR-21表达上调可能与肿瘤进展有关。尿液miR-21联合3D-US可作为筛查BUC的一项有效且无创的诊断方法。

关键词: 尿, 微RNAs, 膀胱肿瘤, microRNA-21, 三维超声成像, 尿路上皮癌

Abstract: Objective　To investigate the clinical value of microRNA-21 (miR-21) expression in urine combined with three-dimensional ultrasound imaging (3D-US) in the diagnosis of urinary urothelial carcinoma (BUC). Methods　The urine samples of 78 BUC patients (the case group) and 80 patients with benign urinary tract diseases (the control group) were collected. The expression level of miR-21 in urine was detected by real-time quantitative PCR (qPCR). The relationship between miR-21 level and clinicopathological characteristics was analyzed. The 3D-US examination was performed, and receiver operating characteristic (ROC) curve was used to analyze the clinical value of urine miR-21 and miR-21 combined with 3D-US for BUC diagnosis. Results　The relative expression level of miR-21 in urine was significantly higher in case group than that of control group (P＜0.01). The content of urine miR-21 was significantly higher in the infiltrating group than that in the non-infiltrating group, and the expression of urine miR-21 was significantly higher in the high-grade group than that in the low-grade group (P＜0.05). ROC curve analysis showed that the sensitivity and specificity of urine miR-21 expression combined with 3D-US were 94.87% and 96.25% in the diagnosis of urothelial carcinoma, and Youden index was close to the result of cystoscopy. Conclusion　The upregulated expression of miR-21 in urine of BUC patients may be related to tumor progression. Urine miR-21 combined with 3D-US can be used as an effective and non-invasive diagnostic method for screening BUC.

Key words: urine, microRNAs, urinary bladder neoplasms, microRNA-21, three-dimensional ultrasonography, urothelium carcinoma