摘要： Objective To explore the effect of thioridazine (TDZ) on proliferation and apoptosis of pancreatic cancer cells SW1990 and its regulatory effect on LINC00470. Methods Different concentrations of TDZ (5, 10 and 20 μmol/L) were used to treat human pancreatic cancer cells SW1990. si-NC and si-LINC00470 were transfected into SW1990 cells (the si-NC group and the si-LINC00470 group). After transfecting pcDNA and pcDNA-LINC00470 into SW1990 cells, TDZ were added to treat cells (the TDZ+pcDNA group and the TDZ+pcDNA-LINC00470 group). MTT, plate clone formation test were used to detect cell proliferation and clone formation ability. Flow cytometry was used to detect the apoptosis rate. The real-time fluorescent quantitative PCR was used to detect the expression level of LINC00470. Western blot assay was used to detect expression levels of activated aspartate specific cysteine protease (cleaved-caspase) 3 and cleaved-caspase9. Results Compared with the control group, the cell proliferation inhibition rate, apoptosis rate, cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased, the number of cell clone formation was decreased, and the expression level of LINC00470 was decreased in the different dose TDZ groups (P<0.05). Compared with the si-NC group, the cell proliferation inhibition rate, apoptosis rate, cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased in the si-LINC00470 group, and the cell clone formation was reduced (P<0.05). The transfection of pcDNA-LINC00470 could reverse the effect of TDZ on cell proliferation, apoptosis and colony formation of SW1990 cells. Conclusion TDZ can inhibit the proliferation, clone formation and induce apoptosis of pancreatic cancer cells by inhibiting the expression of LINC00470.

中图分类号: 

• R735.9，R979.1