期刊目录

2023年, 第51卷, 第3期
刊出日期：2023-03-15 上一期    下一期
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细胞与分子生物学
实验研究
临床研究
应用研究
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细胞与分子生物学

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血管细胞外基质胶促进骨髓CD34+祖细胞分化为内皮细胞的实验研究 左芯萌, 王振华, 高利平 2023, 51 (3):  225-229.  doi: 10.11958/20221138 摘要 ( 686 )   HTML ( 21 )   PDF(1293KB) ( 1098 )   Objective To investigate the role of vascular extracellular matrix (ECM) gel in promoting the differentiation of bone marrow CD34+ progenitor cells into endothelial cells. Methods ECM was obtained from the decellularized aortic tissue, and vascular ECM gel was prepared by enzyme digestion of vascular ECM. HE staining and DAPI staining were used to observe the decellularization effect of aortic tissue. Mouse bone marrow CD34+ progenitor cells were divided into two groups: the fibronectin (FN) group was cultured in plates coated with FN, and the ECM group was cultured in plates coated with human aorta ECM gel at the bottom of the plate. The mRNA expression of CD31, endothelial nitric oxide synthase (eNOS) and Von willebrand factor (VWF) were detected by RT-PCR in both groups. Flow cytometry was used to detect the percentage rates of positive CD31, CD144, vascular endothelial growth factor receptor 2 (VEGFR2), CD133 and CD45 positive cells. The number of phagocytic fluorescein labeled acetylated low-density lipoprotein (DiI-acLDL) cells was observed under fluorescence microscope. The content of proangiogenic factors secreted by cells was detected by protein microarray. Results Both HE and DAPI staining showed that human aortic tissue contained a large number of nuclei before decellularization, while the vascular ECM obtained after decellularization did not contain nuclei. Compared with the FN group, the expression levels of CD31, eNOS and VWF mRNA in CD34+ progenitor cells were increased, the positive rates of CD31, CD144 and VEGFR2 expressing cells and the number of phagocytosing Dil-acLDL cells were also increased in the ECM group, while the positive rates of CD133 and CD45 expressing cells decreased (P<0.05). The secretion of angiopoietin, fibroblast growth factor 7, granulocyte macrophage colony stimulating factor, leptin and platelet-derived growth factor BB were significantly higher in the ECM group than those of the FN group (P<0.05). Conclusion Vascular ECM gel promotes the differentiation of bone marrow CD34+ progenitor cells into endothelial cells, which may provide a new strategy for in situ endothelialization of cardiovascular implants. 数据和表 | 参考文献 | 相关文章 | 计量指标

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硫利达嗪对胰腺癌细胞增殖及凋亡的影响及机制探讨 罗娜, 倪猛, 刁云辉 2023, 51 (3):  230-234.  doi: 10.11958/20220932 摘要 ( 510 )   HTML ( 16 )   PDF(786KB) ( 916 )   Objective To explore the effect of thioridazine (TDZ) on proliferation and apoptosis of pancreatic cancer cells SW1990 and its regulatory effect on LINC00470. Methods Different concentrations of TDZ (5, 10 and 20 μmol/L) were used to treat human pancreatic cancer cells SW1990. si-NC and si-LINC00470 were transfected into SW1990 cells (the si-NC group and the si-LINC00470 group). After transfecting pcDNA and pcDNA-LINC00470 into SW1990 cells, TDZ were added to treat cells (the TDZ+pcDNA group and the TDZ+pcDNA-LINC00470 group). MTT, plate clone formation test were used to detect cell proliferation and clone formation ability. Flow cytometry was used to detect the apoptosis rate. The real-time fluorescent quantitative PCR was used to detect the expression level of LINC00470. Western blot assay was used to detect expression levels of activated aspartate specific cysteine protease (cleaved-caspase) 3 and cleaved-caspase9. Results Compared with the control group, the cell proliferation inhibition rate, apoptosis rate, cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased, the number of cell clone formation was decreased, and the expression level of LINC00470 was decreased in the different dose TDZ groups (P<0.05). Compared with the si-NC group, the cell proliferation inhibition rate, apoptosis rate, cleaved-caspase3 and cleaved-caspase9 protein levels of SW1990 cells were increased in the si-LINC00470 group, and the cell clone formation was reduced (P<0.05). The transfection of pcDNA-LINC00470 could reverse the effect of TDZ on cell proliferation, apoptosis and colony formation of SW1990 cells. Conclusion TDZ can inhibit the proliferation, clone formation and induce apoptosis of pancreatic cancer cells by inhibiting the expression of LINC00470. 数据和表 | 参考文献 | 相关文章 | 计量指标

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金合欢素对肝癌HepG2细胞增殖、凋亡和迁移的影响及机制研究 吴琼, 李锦源, 黄文涛, 安娜 2023, 51 (3):  235-239.  doi: 10.11958/20221115 摘要 ( 630 )   HTML ( 16 )   PDF(1571KB) ( 1126 )   Objective To investigate the effect of acacetin (Aca) on the proliferation, apoptosis and migration of liver cancer HepG2 cells by regulating PTEN induced kinase 1 (PINK1)/E3 ubiquitin protein ligase (Parkin) pathway mediated mitochondrial autophagy. Methods Liver cancer HepG2 cells were divided into the control group (normally cultured HepG2 cells), the Aca group (10 μmol/L Aca), the PINK1 small interfering RNA negative control (si-NC) group (transfected with si-NC), the PINK1 small interfering RNA (si-PINK1) group (transfected with si-PINK1), the Aca+si-NC group (treated with 10 μmol/L Aca after transfection of si-NC) and Aca+si-PINK1 group (treated with 10 μmol/L Aca after transfection of si-PINK1). Cell counting kit-8 (CCK-8) method was used to detect cell proliferation. Flow cytometry was used to detect cell apoptosis. Transwell test was used to detect cell migration. Transmission electron microscope was used to observe the formation of autophagosomes. Western blot assay was used to detect expression levels of autophagy related proteins [Beclin-1, microtubule associated protein 1 light chain 3 (LC3)-Ⅰ and LC3-Ⅱ] and PINK1/Parkin pathway related proteins in cells. Results Compared with the control group, the survival rate and the number of migrating of HepG2 cells were significantly decreased in the Aca group, and the apoptosis rate, number of autophagy bodies, expression levels of Beclin-1, LC3-Ⅱ/LC3-Ⅰ, PINK1 and Parkin protein were increased significantly (P<0.05). The survival rate and the number of migrating of HepG2 cells were significantly increased in the si-PINK1 group, and the apoptosis rate, number of autophagy bodies, expression levels of Beclin-1, LC3-Ⅱ/LC3-Ⅰ, PINK1 and Parkin protein were decreased significantly (P<0.05). Compared with the Aca group and the Aca+si-NC group, the survival rate and the number of migrating of HepG2 cells were significantly increased in the Aca+si-PINK1 group, and the apoptosis rate, number of autophagy bodies, expression levels of Beclin-1, LC3-Ⅱ/LC3-Ⅰ, PINK1 and Parkin protein were decreased significantly (P<0.05). Conclusion Aca may inhibit the proliferation and migration and promote cell apoptosis of liver cancer HepG2 cells by activating mitochondrial autophagy mediated by PINK1/Parkin pathway. 数据和表 | 参考文献 | 相关文章 | 计量指标

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基于PI3K/Akt/mTOR信号通路探讨黄芪多糖对结直肠癌自噬的影响 郅强, 张楠, 冯光玲, 孙维义, 赵媛媛, 杨世发 2023, 51 (3):  240-245.  doi: 10.11958/20220463 摘要 ( 500 )   HTML ( 13 )   PDF(1289KB) ( 1081 )   Objective To investigate the effect and mechanism of astragalus polysaccharides (APS) on autophagy in colorectal cancer. Methods The effects of various concentrations of APS (0.25 g/L, 0.5 g/L, 0.75 g/L and 1 g/L) on the proliferation of human colorectal cancer HCT-116 cells were detected by CCK-8 test. The effect of APS on the migration of HCT-116 cells was detected by cell scratch assay. The effect of APS on autophagy of colorectal cancer cells was detected by dansylcadaverine staining technique. Effects of APS on autophagy and PI3K/Akt/mTOR signaling pathway related proteins LC3B, p62, p-PI3K, p-Akt, p-mTOR, PI3K, Akt and mTOR in HCT-116 cells and colorectal cancer tumor-bearing nude mice were detected by Western blot assay. The expression of autophagy-related protein p62 in colorectal cancer tumors was detected by immunohistochemistry. Results APS inhibited HCT-116 cell proliferation in a dose-dependent manner (P<0.05). APS could inhibit the proliferation and migration of HCT-116 cells by inducing autophagy (P<0.05). The autophagy inhibitor 3-MA (2 mmol/L) not only attenuated the autophagy induction effect of APS on HCT-116 cells, but also attenuated the inhibitory effect of APS on the proliferation and migration of HCT-116 cells (P<0.05). APS could increase the expression ratio of autophagy-related protein LC3BⅡ/Ⅰ and reduce the expression level of p62 protein in HCT-116 cells and colorectal cancer mice (P<0.05). 3-MA could attenuate the increasing effect of APS on the expression ratio of LC3BⅡ/Ⅰ protein and reduce the inhibitory effect of APS on the expression of p62 protein in HCT-116 cells (P<0.05). APS could reduce the expression ratio of PI3K/Akt/mTOR signaling pathway related proteins p-PI3K/PI3K, p-Akt/Akt and p-mTOR/mTOR in HCT-116 cells and colorectal cancer tumor (P<0.05). The pathway agonist 740Y-P (10 μmol/L) could attenuate the inhibitory effect of APS on expression levels of PI3K/Akt/mTOR signaling pathway-related proteins in HCT-116 cells (P<0.05). Conclusion APS can induce autophagy activation in colorectal cancer tumor tissue and cells, and inhibit cell proliferation and migration of HCT-116 cells by inducing autophagy. The mechanism of action may be related to the inhibition of PI3K/Akt/mTOR signaling pathway. 数据和表 | 参考文献 | 相关文章 | 计量指标

实验研究

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转录因子EB在衰老心肌细胞自噬中的作用机制研究 盛思琪, 谢琳, 姜怡邓, 熊建团, 杨安宁, 吴凯, 杨勇, 杨晓明 2023, 51 (3):  246-251.  doi: 10.11958/20220920 摘要 ( 519 )   HTML ( 12 )   PDF(928KB) ( 1045 )   Objective To explore the mechanism of transcription factor EB (TFEB) in autophagy of aging cardiomyocytes. Methods Animal experiment: Twenty aged Wistar rats were randomly divided into the sham operation group (Sham group) and the ischemia-reperfusion injury group (I/R group). Cell experiments: (1) Aging cardiomyocytes were cultured in vitro, incubated with 8 g/L D-galactose for 8 days, and then divided into the normoxia group and the hypoxia/reoxygenation group (H/R group). (2) Aging cardiomyocytes transfected by adenovirus overexpressing and interfering with TFEB were divided into the Ad-GFP group, the Ad-GFP+H/R group, the Ad-TFEB group, the Ad-TFEB+H/R group, the sh-NC group, the sh-NC+H/R group, the sh-TFEB group and the sh-TFEB+H/R group. (3) Aging cardiomyocytes treated with specific inhibitors of DNMT1, DNMT3a and DNMT3b after hypoxia/reoxygenation were divided into the H/R group, the DNMT1 specific inhibitor (DC-05) group, the DNMT3a specific inhibitor (TFD) group and the DNMT3b specific inhibitor (NA) group. (4) Aging cardiomyocytes transfected by adenovirus interfering with DNMT3b were divided into the sh-NC group, the sh-NC+H/R group, the sh-DNMT3b group and the sh-DNMT3b+H/R group. Quantitative real-time PCR (qPCR) was used to detect the mRNA level of TFEB, and Western blot assay was used to detect the autophagy related proteins TFEB, LC3B and p62 in aging cardiomyocytes. The DNA methylation levels of TFEB promoter in aging myocardium and cardiomyocytes were detected by nested methylation specific PCR (nMS-PCR). Results Compared with the Sham group or the normoxia group, the mRNA and protein expression of TFEB were decreased in the I/R group and the H/R group (P<0.01). The protein expression of LC3B-Ⅱ/Ⅰ was decreased in aging cardiomyocytes after overexpression of TFEB in the Ad-TFEB group compared with the Ad-GFP group, while the protein expression of p62 was increased (P<0.01). The opposite results were obtained after interfering with TFEB (P<0.01). Compared with the Sham group or the normoxia group, the DNA methylation level of the TFEB promoter was increased in the I/R group and the H/R group (P<0.05). Compared with the H/R group, it was found that the mRNA and protein expression level of TFEB were increased in the NA group (P<0.01). And the TFEB mRNA and protein expression were increased in aging cardiomyocytes after overexpressed interference with DNMT3b (P<0.01). Conclusion DNMT3b inhibits the TFEB expression by regulating DNA methylation of TFEB promoter, thus to promote autophagy of aging cardiomyocytes. 数据和表 | 参考文献 | 相关文章 | 计量指标

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杜仲叶总黄酮通过RhoA/ROCK信号通路参与脑出血大鼠神经功能修复 张秀峰, 李小菲, 刘明, 王慧静 2023, 51 (3):  252-258.  doi: 10.11958/20221033 摘要 ( 582 )   HTML ( 10 )   PDF(1203KB) ( 2322 )   Objective To explore the possible mechanism of eucommia ulmoides leaves total flavonoids promoting neurological repair in rats with cerebral hemorrhage from the Ras homolog gene family member A (RhoA)/Rho-associated coiled-coil protein kinase (ROCK) pathway. Methods SD male rats were used to establish cerebral hemorrhage model by the modified double injection method, and they were randomly divided into the sham operation group, the model group, the eucommia ulmoides leaves total flavonoids group, the RhoA inhibitor group, the RhoA agonist group, and the eucommia ulmoides leaves total flavonoids + RhoA agonist group, with 20 rats in each group. The eucommia ulmoides leaves total flavonoids group was intragaically treated with 200 mg/kg eucommia ulmoides leaves total flavonoids. The RhoA inhibitor group was intraperitoneally injected with 1 mg/kg RhoA inhibitor Y27632. The RhoA agonist group was intraperitoneally injected with 30 μg/kg RhoA agonist U-46619, and the eucommia ulmoides leaves total flavonoids + RhoA agonist group was intraperitoneally injected with 200 mg/kg eucommia ulmoides leaves total flavonoids and 30 μg/kg RhoA agonist U-46619. The model group and sham operation group were given 10 mL/kg normal saline once a day for 7 consecutive days. After the administration, the behavioral changes of rats were observed with the naked eye and the neurological deficit symptoms were assessed by the modified neurological severity score (mNSS). Brain tissue was taken and weighed, and the water content of the brain tissue was calculated according to the formula to evaluate the cerebral edema, then the brain tissue slices were prepared and the volume percentage of the cerebral hematoma was calculated according to the formula. Changes in the structure of nerve synapses in perihematoma tissue were observed by transmission electron microscope. Changes of neuronal apoptosis of perihematoma tissue were observed by TUNEL staining method. Changes in neuron skeleton of perihematoma tissue were detected by phalloidin staining. The expression levels of RhoA, ROCK, cytoskeleton protein (F-actin), filament protein (cofilin), phosphorylated cofilin (p-cofilin), neuron and synapse growth promoting proteins [nerve growth factor (NGF), neurotrophic factor 3 (NT3), postsynaptic dense protein-95 (PSD-95), synaptophysin (SYP)] in perihematoma tissue were detected by Western blot assay. Results Compared with the sham operation group, the neurological deficit scores, water content of brain tissue and the percentage of cerebral hematoma volume were increased in the model group, the neuronal apoptosis, skeleton structure damage, and changes in the structure of nerve synapses in the surrounding tissue of the hematoma were severer, the activation of RhoA/ROCK pathway increased, and the expression of neuron and synapse growth promoting proteins decreased (P<0.05). Eucommia ulmoides leaves total flavonoids or RhoA inhibitor intervention treatment could inhibit changes in neuron skeleton structure mediated by RhoA/ROCK pathway activation, increase the expression of neuron and synapse growth promoting proteins, alleviate neuronal damage and apoptosis in surrounding tissues of the hematoma after cerebral hemorrhage, and promote nerve function repair (P<0.05). RhoA agonist could promote the activation of RhoA/ROCK pathway, aggravate the neurological damage after cerebral hemorrhage, and weaken the nerve function repairing effect of eucommia ulmoides leaves total flavonoids (P<0.05). Conclusion Eucommia ulmoides leaves total flavonoids can improve the symptoms of cerebral hemorrhage in rats and promote the nerve function repair by inhibiting the activation of RhoA/ROCK pathway. 数据和表 | 参考文献 | 相关文章 | 计量指标

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紫杉醇对实验性自身免疫性脑脊髓炎（EAE）小鼠细胞焦亡的影响及其机制的研究 潘婷, 李作孝, 晚丽 2023, 51 (3):  259-262.  doi: 10.11958/20221146 摘要 ( 575 )   HTML ( 12 )   PDF(1285KB) ( 1052 )   Objective To investigate the effect of taxol (PTX) on pyroptosis of experimental autoimmune encephalomyelitis (EAE) mice by inhibiting NOD-like receptor thermoprotein domain-associated protein 3 (NLRP3)/caspase-1 pathway. Methods Fifty female C57BL/6 mice were randomly divided into the normal control group, the EAE model group, the PTX low-dose, medium-dose and high-dose groups according to the random number table method, with 10 mice in each group. EAE model was established in other groups except the normal control group. PTX low-dose, medium-dose and high-dose groups were intraperitoneally injected with 1, 2 and 4 mg/kg PTX everyday, respectively, and normal control group and EAE model group were intraperitoneally injected with the same amount of normal saline for 14 consecutive days. From the day of immunization, changes of body weight, mental state, activity state and neurological dysfunction score of mice were observed and recorded regularly every day. Nissl staining and LFB staining were used to observe pathological changes. The mRNA expression levels of NLRP3 and caspase-1 in crushed tissues were detected by quantitative fluorescence PCR (qPCR). Levels of interleukin (IL) -18 and IL-1β in peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA). Results There were no obvious symptoms in mice of the normal control group. Mice in the EAE model group and the PTX low-, medium-, and high-dose groups showed decreased body weight, decreased activity and sluggish response. Compared with the EAE model group, the latency and peak period were delayed in the PTX dose groups, the neurological dysfunction score at peak period was decreased (P<0.05). The number of Nyssome in spinal cord tissue was increased and relatively regular, demyelination and vacuolar changes were alleviated, and the mRNA expression levels of NLRP3 and caspase-1 were decreased. The higher the PTX dose, the more obvious the latency and peak delay. The number of Nyssome in spinal cord increased, the arrangement and morphology were more regular, the demyelination and vacuolation changes were lighter, the mRNA expression level of caspase-1 and the contents of IL-1β and IL-18 in peripheral blood were lower (P<0.05). Conclusion PTX may reduce the damage of pyroptosis in EAE mice by inhibiting NLRP3/caspase-1 pathway related inflammatory factors. 数据和表 | 参考文献 | 相关文章 | 计量指标

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逍遥散通过调节肿瘤免疫微环境抑制肺转移前微环境形成 汤晓霞, 邓皖利, 张勇, 陈彬, 柴妮, 张澍, 谢曼丽 2023, 51 (3):  263-268.  doi: 10.11958/20220136 摘要 ( 450 )   HTML ( 6 )   PDF(1114KB) ( 1361 )   Objective To investigate the mechanism of XiaoYaoSan in anti-tumor metastasis. Methods A mouse model of CT26.WT colon cancer xenograft and lung metastasis was established. In vivo imaging of small animals, lung tissue anatomy and HE staining were performed to evaluate the lung metastasis under different doses (200, 400 and 800 mg) of XiaoYaoSan intervention. Tumor M2 macrophage markers were detected by immunofluorescence and Western blot assay. Serum levels of transforming growth factor (TGF) -β and tumor necrosis factor (TNF) -α were detected by enzyme-linked immunosorbent assay (ELISA). The recruitment of VEGFR1+ myeloid cells in lung tissue was detected by flow cytometry. The expression levels of phosphatidylinositol 3-kinase (STAT3), vascular endothelial growth factor (VEGF) and pre-metastasis microenvironment markers matrix metalloproteinase (MMP) -9, S100A8 and Fibronectin were detected by Western blot assay. Results After XiaoYaoSan intervention, the lung metastasis was significantly reduced, expression levels of MMP-9, S100A8 and Fibronectin in lung tissue were significantly decreased, M1 polarization occurred in tumor-associated macrophages, the serum level of TGF-β was significantly decreased, and TNF-α level was significantly increased. The recruitment of VEGFR1+ myeloid cells was significantly decreased in lung tissue, STAT3 phosphorylation was inhibited, and VEGF expression was significantly decreased. Conclusion The antimetastatic activity of XiaoYaoSan is related to its ability to inhibit the pre-metastatic microenvironment, which may be realized by the phenotypic transformation of tumor-associated macrophages and inhibition of STAT3 signal transduction. 数据和表 | 参考文献 | 相关文章 | 计量指标

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丹参多酚酸对免疫性不孕大鼠的治疗作用及对下丘脑-垂体-卵巢轴的影响 柳艾霞, 张庆玲, 武志娟 2023, 51 (3):  269-273.  doi: 10.11958/20220990 摘要 ( 445 )   HTML ( 7 )   PDF(1194KB) ( 1053 )   Objective To investigate the therapeutic effect of salvia miltiorrhiza polyphenolic acid on immune infertility rats and its effect on hypothalamus-pituitary-ovarian axis (HPOA). Methods Fifty non-pregnant female SD rats were selected. A rat model of immune infertility with anti-sperm antibody (+) was established by human semen injection immunization. The model rats were divided into the model group (n=9), the prednisone group (n=10), the alvia miltiorrhiza polyphenolic acid low dose group (n=9), the salvia miltiorrhiza polyphenolic acid medium dose group (n=10) and the salvia miltiorrhiza polyphenolic acid high dose group (n=10) by random array table method. Another 10 non-pregnant female rats were selected as the normal control group. The prednisone group was given 5 mg/kg prednisone by intraperitoneal injection, and the salvia miltiorrhiza polyphenolic acid low, medium and high dose groups were given 10, 20 and 40 mg/kg salvia miltiorrhiza polyphenolic acid by intraperitoneal injection. The normal control group and model group were given the same amount of normal saline, once a day for 14 days. The female rats and normal male rats in each group were caged in a ratio of 3∶1 for 2 weeks, and the pregnancy rate of rats in each group was observed. The serum levels of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) were determined by radioimmunoassay. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of rat ovarian tissue. Real-time quantitative reverse transcription polymerase chain reaction (qPCR) was used to detect rat ovarian follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and estrogen receptor alpha (ERα) messenger ribonucleic acid (mRNA) expression. Western blotting (WB) was used to detect rat ovarian FSHR, LHR and ERα protein expressions. Results Compared with the normal control group, the conception rate and the serum levels of GnRH, FSH, LH and E2 were decreased in the model group (P<0.05), the corpus luteum and mature follicles in the ovary were significantly reduced, the number of granulosa cell layers was reduced, and there were more cystic follicles, and the mRNA and protein expressions of FSHR, LHR and ERα were decreased (P<0.05). Compared with the model group, the conception rate and the serum levels of GnRH, FSH, LH, and E2 were increased in the prednisone group and the salvia miltiorrhiza polyphenolic acid medium and high dose groups (P<0.05), the number of cystic follicles was significantly decreased, the number of granulosa cell layers was increased, and mature follicles and corpus luteum were formed. The mRNA and protein expressions of FSHR, LHR and ERα were increased (P<0.05). Conclusion Salvia miltiorrhiza polyphenolic acid can promote follicle maturation by regulating HPOA in the treatment of immune infertility. 数据和表 | 参考文献 | 相关文章 | 计量指标

临床研究

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氢吗啡酮复合罗哌卡因骶管阻滞在儿童尿道下裂手术中的应用效果 张萌, 覃道锐, 杨博, 谢敏, 刘云飞, 苏鹏 2023, 51 (3):  274-276.  doi: 10.11958/20221210 摘要 ( 504 )   HTML ( 12 )   PDF(681KB) ( 1052 )   Objective To evaluate the efficacy and safety of ropivacaine combined with hydromorphone in caudal block for children undergoing hypospadias surgery. Methods A total of 110 male children aged 0-4 years old who underwent hypospadias surgery were randomly divided into the R group (received caudal block with 0.2% ropivacaine 1 mL/kg) and the HR group (received caudal block with 0.2% ropivacaine 1 mL/kg and hydromorphone 10 μg/kg) with 55 children for each group. Anesthesia time, operation time, postoperative Children's Hospital of Eastern Ontario Pain Scale (CHEOPS) pain scores and complications were recorded and compared between the two groups. Results Compared with the R group, the duration of analgesia time was longer in the HR group (P<0.05), and the 12 h and 18 h CHEOPS pain scores after surgery were lower in the HR group (P<0.05). There were no significant differences in the postoperative complications between the two groups (P>0.05). Conclusion The utilization of ropivacaine combined with hydromorphone in caudal block is safe and effective in children undergoing hypospadias surgery. This method could significantly prolong analgesic time of caudal block and improve the postoperative recovery quality. 数据和表 | 参考文献 | 相关文章 | 计量指标

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不同降压模式在老年高血压合并冠心病患者PCI术后的应用效果研究 陆荣臻, 黄江南, 潘兴寿, 陆婷, 李近都, 李天资, 谭舒韩 2023, 51 (3):  277-281.  doi: 10.11958/20221100 摘要 ( 430 )   HTML ( 16 )   PDF(711KB) ( 1097 )   Objective To investigate the efficacy and safety of intensive antihypertensive therapy and standard antihypertensive therapy after percutaneous coronary intervention (PCI) in elderly hypertensive patients with coronary heart disease. Methods A total of 86 elderly patients with hypertension complicated with coronary heart disease were randomly divided into the control group and the study group with 43 cases in each group. The control group was given standard antihypertensive therapy [target systolic blood pressure (SBP)<135 mmHg], and the study group was given intensive antihypertensive therapy target SBP<120 mmHg). Follow-up was conducted for 12 months from the beginning of treatment. The variation rate of blood pressure before and after treatment was recorded by wearing a home blood pressure monitor. Color Doppler ultrasound was used to evaluate the cardiac function indexes before and after treatment. The visual analogue scale of the European Five-Dimensional Health Assessment Scale (EQ-5D-3L) was used to evaluate the health status of patients. The patient Health Questionnaire Depression Scale (PHQ-9) was used to evaluate the depression mood of the patients. The Self-rating Anxiety Scale (SAS) was used to evaluate anxiety mood, and the fall Efficacy Scale International version (FES-I) was used to evaluate fall risk. Results There were no significant differences in 24 h SBP and 24 h diastolic blood pressure (DBP) coefficient of variation, left ventricular ejection fraction (LVEF), left ventricular mass index (LVMI), left atrial volume index (LAVI), EQ-5D-3L score, PHQ-9 score, SAS score and FES-I score before treatment between the two groups (P>0.05). The coefficients of variation of 24 h SBP and 24 h DBP were lower in the study group than those in the control group (P<0.05). Data of LVEF, LVMI and EQ-5D-3L were higher in the study group than those in the control group (P<0.05). LAVI, PHQ-9, SAS and FES-I were lower than those in the control group (P<0.05). There was no significant difference in the incidence of total adverse reactions between the study group and the control group (P>0.05). The total incidence of new cardiovascular and cerebrovascular events were lower in the study group than those in the control group (P<0.05). Conclusion Intensive antihypertensive therapy is better than standard antihypertensive therapy in controlling blood pressure (24 h) rhythm variability and cardiac function after PCI in elderly hypertensive patients with coronary heart disease and can reduce the risk of cardiovascular and cerebrovascular adverse events, improve the quality of life with good safety. 数据和表 | 参考文献 | 相关文章 | 计量指标

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允许性高碳酸血症对老年胸腔镜食管癌根治术患者术后谵妄的影响 邵艳梅, 宋洁, 杨梦思, 黄孝慈, 汤昕宇, 胡宪文 2023, 51 (3):  282-285.  doi: 10.11958/20220699 摘要 ( 493 )   HTML ( 13 )   PDF(700KB) ( 1080 )   Objective To investigate the effect of permissible hypercapnia (PHY) on postoperative delirium (POD) in elderly patients undergoing thoracoscopic radical resection of esophageal cancer. Methods A total of 92 elderly patients (≥65 years old) who underwent thoracoscopic radical resection of esophageal cancer were randomly divided into the normal arterial partial pressure of carbon dioxide [p(CO2) was controlled at 35-45 mmHg] group (N group, 46 cases) and the mild PHY [p(CO2) was controlled at 46-55 mmHg] group (H group, 46 cases). Data of mean arterial pressure (MAP), heart rate (HR) and cerebral oxygen saturation (rSO2) were recorded when patients were awake (T0), intubated (T1), pneumothorax 0.5 h (T2), pneumothorax 1 h (T3) and vented for 10 min (T4). Intraoperative fluid volume, urine volume, blood loss and ephedrine dosage were recorded in the two groups of patients. Visual analogue scale (VAS) was recorded for three consecutive days after operation, and the occurrence of POD was assessed by Delirium Diagnostic Scale (CAM). Results Compared with T0, MAP, HR and rSO2 were decreased at T1-T4 in the N group and the H group (P<0.05). Compared with the group N, MAP and rSO2 were higher at T2-T4 in the group H (P<0.05). Correlation analysis showed that rSO2 was positively correlated with p(CO2) at T2-T4 in the two groups of patients (rs=0.438, 0.646 and 0.554, all P<0.01). There were no significant differences in infusion volume, urine volume and blood loss between the two groups of patients (P>0.05). Compared with the group N, the amount of ephedrine was lower in the group H [(5.8±3.2) mg vs. (4.0±2.5) mg, t=3.014, P<0.01]. The VAS score were gradually decreased 3 days after operation in the two groups (P<0.05), and there was no significant difference in VAS scores between the two groups (P>0.05). Compared with the group N, the incidence of POD was lower in the group H (8.7% vs. 23.9%, χ2=3.903, P<0.05). Conclusion PHY ventilation strategy in elderly patients undergoing thoracoscopic radical resection of esophageal cancer is helpful to optimize the balance of cerebral oxygen supply and demand, increase rSO2, and reduce the incidence of POD. 数据和表 | 参考文献 | 相关文章 | 计量指标

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改良胸腰筋膜间平面阻滞对腰椎减压融合术患者术后恢复质量的影响 孙高悦, 钟晓倩, 张倩倩, 李云 2023, 51 (3):  286-289.  doi: 10.11958/20221185 摘要 ( 445 )   HTML ( 5 )   PDF(697KB) ( 1174 )   Objective To evaluate the effect of ultrasound-guided modified thoracolumbar interfascial plane (mTLIP) block on the quality of recovery (QoR) in patients undergoing lumbar decompression and fusion. Methods Sixty patients undergoing lumbar decompression and fusion were divided into two groups (n=30) by a random number table method: the mTLIP block group (group T) and the control group (group C). The patients in the group T were injected with 0.4% ropivacaine 20 mL+20 μg dexmedetomidine between longissimus and iliocostalis muscles at the third lumbar spine vertebral level on each side under ultrasound guidance, while patients in the group C were not received nerve block. All patients received patient controlled intravenous analgesia (PCIA) after operation. When patients had a postoperative pain numerical rating scale (NRS) ≥4, flurbiprofen ester 50 mg was given intravenously for remedial analgesia. The QoR-15 scores of patients in both groups were recorded before and 24 hours after operative. The NRS scores for pain in both groups were recorded at 0.5, 2, 6, 12, 24 and 48 hours postoperatively. The area under curve (AUC) of NRS scores at rest and at activity at 24 hours after surgery, the patient satisfaction scores, PCIA press times and the use of remedial analgesia at 24 h after operation, postoperative nausea and vomiting were also recorded. Results Compared with the group C, patients in the group T had higher QoR-15 scores at 24 hours postoperatively, higher patient satisfaction scores, lower NRS scores at rest and at activity at 0.5 h, 2 h, 12 h, 24 h and 48 h postoperatively, lower AUC, fewer PCIA press times and lower rate of remedial analgesia at rest and at activity (P<0.05). Conclusion Ultrasound-guided dexmedetomidine compounded with ropivacaine for modified thoracolumbar fascial plane nerve block can improve the quality of postoperative recovery in patients undergoing lumbar decompression and fusion, relieve postoperative pain, improve patient satisfaction and promote early postoperative recovery. 数据和表 | 参考文献 | 相关文章 | 计量指标

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预后营养指数联合NLR、PLR在腹膜透析患者全因死亡中的预测价值 刘志超, 徐可, 布海霞, 窦现凤 2023, 51 (3):  290-293.  doi: 10.11958/20221230 摘要 ( 519 )   HTML ( 8 )   PDF(875KB) ( 1114 )   Objective To investigate the clinical value of prognostic nutritional index (PNI), neutrophil to lymphocyte ratio (NLR) and platelet to lymphocyte ratio (PLR) in all-cause mortality of patients with peritoneal dialysis (PD). Methods A total of 189 patients who received PD were divided into the death group (n=71) and the survival group (n=118) according to whether they died during follow-up. The differences of clinical indicators between the two groups were compared. The clinical value of PNI score, NLR and PLR in predicting the death of PD patients was analyzed by receiver operating characteristic (ROC) curve. Multivariate Cox regression model was used to analyze the risk factors for death. Kaplan-Meier method was used to compare the survival differences of patients with different PNI scores, NLR and PLR levels. Results Compared with the survival group, patients were older, the proportion of patients with hypertension and diabetes increased, the NLR and PLR were increased, and the PNI was decreased in the death group (P<0.05). The ROC curve results showed that the area under the ROC curve (AUC) of NLR, PLR and PNI in predicting all-cause death in peritoneal dialysis patients were 0.684, 0.689 and 0.729, respectively, and the sensitivity were 57.75%, 67.61% and 57.75%, respectively. The specificity were 79.66%, 68.64%, 80.51%, and the optimal cut-off values were NLR=4.91, PLR=152.92, PNI=43.74, respectively. Cox regression analysis showed that increased NLR (HR=2.271, 95%CI: 1.375-3.753), increased PLR (HR=2.846, 95%CI: 1.672-4.844) and decreased PNI (HR=2.802, 95% CI: 1.701-4.616) were risk factors for all-cause mortality in PD patients. Survival analysis showed that the cumulative survival rates of patients with NLR≥4.91, PLR≥152.92 and PNI≤43.74 were significantly decreased (Log rank χ2= 23.299, 20.625, 28.183, P<0.01). Conclusion PNI combined with NLR and PLR has a good predictive effect on the prognosis of PD patients. 数据和表 | 参考文献 | 相关文章 | 计量指标

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sTIM-3及其配体Gal-9、HMGB1与2型糖尿病并发冠心病的相关性研究 刘艳秋, 范海迪, 侯海燕, 孙健, 林宁 2023, 51 (3):  294-298.  doi: 10.11958/20221307 摘要 ( 452 )   HTML ( 8 )   PDF(776KB) ( 1044 )   Objective To investigate the relationship between serum soluble T cell immunoglobulin and mucin domain-3 (sTIM-3), galectin-9 (Gal-9) and high mobility group protein B1 (HMGB1) levels in patients with type 2 diabetes mellitus (T2DM) complicated with coronary heart disease (CHD). Methods Fifty patients with T2DM were used as the T2DM group, and fifty-two patients with T2DM complicated with CHD were selected as the T2DM+CHD group. Forty-eight healthy physical examination subjects were selected as the control group (Con group). Serum levels of sTIM-3, Gal-9 and HMGB1 were detected by enzyme-linked immunosorbent assay (ELISA). Spearman correlation analysis was used to analyze the correlation between serum sTIM-3, Gal-9, HMGB1, fasting blood glucose (FBG) and high-sensitivity C-reactive protein (hs-CRP). Receiver operating characteristic curve (ROC) was used to analyse the diagnostic capability of sTIM-3, Gal-9 and their combination in T2DM complicated with CHD. Logistic regression was used to investigate the risk factors of T2DM complicated with CHD. Results Compared with the Con group, sTIM-3, Gal-9 and HMGB1 were significantly increased in the T2DM group and the T2DM+CHD group (P<0.05). Compared with the T2DM group, sTIM-3 and Gal-9 were significantly increased in the T2DM+CHD group (P<0.05), and there was no significant difference in HMGB1. FBG and hs-CRP were positively correlated with sTIM-3, Gal-9 and HMGB1, sTIM-3 was positively correlated with Gal-9 and HMGB1, and Gal-9 was positively correlated with HMGB1 (P<0.05). ROC curve analysis showed that the area under the curve of serum sTIM-3 and Gal-9 in the diagnosis of T2DM complicated with CHD was 0.812 (95%CI: 0.743-0.882). Logistic regression analysis showed that increased body mass index, sTIM-3 and Gal-9 were risk factors for T2DM complicated with CHD. Conclusion Increased serum levels of sTIM-3 and Gal-9 are risk factors for T2DM complicated with CHD. 数据和表 | 参考文献 | 相关文章 | 计量指标

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急性时相反应蛋白SAA和CRP对川崎病静脉注射免疫球蛋白无应答的预测价值 黄晓碧, 赵胜, 郑丽云, 祁晓慧 2023, 51 (3):  299-302.  doi: 10.11958/20221011 摘要 ( 500 )   HTML ( 12 )   PDF(735KB) ( 1078 )   Objective To evaluate the predictive value of acute phase proteins serum amyloid A protein (SAA) and C-reactive protein (CRP) for non-response to intravenous immunoglobulin (IVIG) in children with Kawasaki disease (KD). Methods According to the IVIG resistant definition, a total of 358 KD patients were assigned into the IVIG resistant group (n=41) and the IVIG responsive group (n=317). Serum levels of SAA and CRP were tested, and SAA/CRP ratio was calculated. Clinical and laboratory data before and after treatment were compared between the two groups. Binary Logistic regression analysis was used to identify influencing factors for resistance to IVIG. The diagnostic value of SAA and CRP in predicting IVIG resistance in KD was investigated by the receiver operating characteristic (ROC) curve. Results The levels of CRP, SAA, total bilirubin, alanine aminotransferase and asparate aminotransferase were significantly higher in the IVIG resistant group than those of the IVIG responsive group (P<0.05), whereas levels of lymphocyte count, platelet count, serum sodium and duration of fever before IVIG were significantly lower in the IVIG resistant group (P<0.05). The binary Logistic regression analysis showed that CRP (OR=1.008, 95%CI: 1.001-1.015), SAA (OR=1.002, 95%CI: 1.001-1.003), total bilirubin (OR=1.030, 95%CI: 1.009-1.051), serum sodium (OR=0.862, 95%CI: 0.762-0.975), lymphocyte count (OR=0.733, 95%CI: 0.567-0.947) and duration of fever before IVIG (OR=0.688, 95%CI: 0.513-0.922) were the independent influencing factors for IVIG resistance (P<0.05). The incidence of coronary artery lesion was significantly higher after therapy in the IVIG resistant group than that in the IVIG responsive group (P<0.05). ROC curve showed that the Youden index of SAA (cut-off value 252.45 mg/L), CRP (cut-off value 82.80 mg/L) and combined SAA and CRP were 0.325, 0.382 and 0.423. The area under the ROC curve of SAA, CRP and two items were 0.681, 0.703 and 0.761, respectively. The predictive efficiency of combined application was larger. Conclusion The increased SAA and CRP levels are independent risk factors of IVIG resistance, which can be utilized as combined biomarkers for the prediction of IVIG resistance in KD patients. 数据和表 | 参考文献 | 相关文章 | 计量指标

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血清Sestrin2在急性ST段抬高型心肌梗死中的表达及其与缺血再灌注损伤的关系 滕丽峰, 陈茜茜, 叶丛, 黄玉冰 2023, 51 (3):  303-306.  doi: 10.11958/20220813 摘要 ( 523 )   HTML ( 14 )   PDF(729KB) ( 1039 )   Objective To investigate the expression of serum Sestrin2 in acute ST-segment elevation myocardial infarction (STEMI) and its relationship with ischemia-reperfusion injury (IRI). Methods A total of 124 STEMI patients were selected as the STEMI group, and they were divided into the IRI group (72 cases) and the non-IRI group (52 cases) according to whether IRI occurred after surgery. Another 50 healthy volunteers were selected as the control group. Serum levels of Sestrin2, malondialdehyde (MDA) and superoxide dismutase (SOD) were detected in all subjects. Pearson's method was used to analyze the correlation between serum Sestrin2 and other indicators in STEMI patients. Multivariate Logistic regression analysis was used to analyze the risk factors of IRI in STEMI patients. The receiver operating characteristic curve (ROC) was used to analyze the predictive value of relevant indicators for the occurrence of IRI in STEMI patients. Results The serum levels of Sestrin2 and MDA were significantly higher in the STEMI group than those in the control group, and the serum level of SOD was significantly lower than that in the control group (P<0.01). The serum levels of Sestrin2, MDA and cardiac troponin (cTnI) were significantly higher in the IRI group than those of the non-IRI group, and the serum SOD level was significantly lower than that of the non-IRI group (P<0.01). Serum Sestrin2 level was positively correlated with MDA level in STEMI patients, and it was negatively correlated with serum SOD level (P<0.05). Multivariate Logistic regression analysis showed that high serum levels of Sestrin2 and MDA were risk factors for IRI in STEMI patients, while high SOD level was protective factors (P<0.05). The ROC analysis showed that the areas under the curve of serum Sestrin2, MDA and SOD for predicting the risk of IRI in STEMI patients were 0.759 (95%CI: 0.673-0.845), 0.654 (95%CI: 0.557-0.751) and 0.671 (95% CI: 0.575-0.767), respectively, and serum Sestrin2 had the highest predictive value. Conclusion The expression of Sestrin2 is abnormally high in STEMI patients, and its expression level is closely related to the occurrence of IRI in patients. 数据和表 | 参考文献 | 相关文章 | 计量指标

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血清CTRP1、CTRP5和CTRP1/CTRP5水平与不稳定型心绞痛的关系 杨宏秀, 袁宝军, 刘丽, 赵莹, 张菲菲, 佟艳艳, 张会芬 2023, 51 (3):  307-310.  doi: 10.11958/20220680 摘要 ( 321 )   HTML ( 13 )   PDF(760KB) ( 1152 )   Objective To investigate the relationship between serum complement- C1q/TNF related protein 1 (CTRP1), CTRP5 and ratio of CTRP1 to CTRP5 (CTRP1/CTRP5) and unstable angina pectoris (UAP). Methods A total of 103 patients with UAP were selected as subjects. Patients were divided into the mild coronary stenosis group (GS≤39, n=53) and the severe coronary stenosis group (GS>39, n=50) according to the median of Gensini (GS) score. Patients were divided into the single-vessel lesion group (n=20), the double-vessel lesion group (n=46) and the multi-vessel lesion group (n=37), according to the number of coronary artery lesions. Forty-seven patients with cardiac neurosis whose coronary stenosis was less than 50% were selected as the control group. Enzyme linked immunosorbent assay was used to detect serum levels of CTRP1 and CTRP5, and then the level of CTRP1/CTRP5 was calculated. Results The levels of CTRP1, CTRP5 and CTRP1/CTRP5 were higher in the UAP group than those in the control group (P<0.05). The levels of CTRP1 and CTRP5 were higher in the severe coronary stenosis group than those of the mild coronary stenosis group (P<0.05). The level of CTRP1 was higher in the multi-vessel lesion group than that in the single-vessel lesion group among UAP with different vascular lesions (P<0.05). Results of multivariate Logistic analysis showed that CTRP1 was an independent predictor of UAP. ROC curve analysis showed that the area under the ROC curve (AUC) of serum CTRP1, CTRP5 and CTRP1/CTRP5 for UAP assisted screening were 0.757, 0.631 and 0.625, and the cut-off values were 870.33 μg/L, 43.04 μg/L and 18.41, respectively. The sensitivity of CTRP1/CTRP5 was higher (84.5%), and the specificity of CTRP1 was higher (78.7%). Conclusion CTRP1 and CTRP5 are involved in the occurrence and development of UAP. Detection of serum levels of CTRP1 and CTRP5 and calculation of CTRP1/CTRP5 levels are helpful for UAP screening and disease assessment. 数据和表 | 参考文献 | 相关文章 | 计量指标

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脓毒症所致的ALI/ARDS患者血浆SIRT-1、syndecan-1的表达水平及其对预后的影响 冯颂乔, 何业伟, 王妍 2023, 51 (3):  311-314.  doi: 10.11958/20221108 摘要 ( 540 )   HTML ( 10 )   PDF(726KB) ( 1098 )   Objective To analyze plasma expression levels of silent information regulation 1 (SIRT-1) and syndecan-1 in patients with acute lung injury (ALI)/ associated acute respiratory distress syndrome (ARDS) and their correlation with prognosis. Methods A total of 106 patients with ALI/ARDS were selected as the ALI/ARDS group. The survival status of all patients within one month after admission was counted, and they were divided into the survival group (66 cases) and the death group (40 cases) according to their survival status. In addition, 50 healthy volunteers were selected as the control group. Expression levels of plasma SIRT-1 and syndecan-1 of all subjects were detected by enzyme-linked immunosorbent assay. Clinical data of acute physiology and chronic health score Ⅱ (APACHE Ⅱ) and lung injury score (LIS) were collected. Results The plasma SIRT-1 level was lower in the ALI/ARDS group than that in the control group, and the plasma syndecan-1 level was higher than that in the control group (P<0.05). The plasma SIRT-1 level was lower in the death group than that in the surviva group, and APACHE Ⅱ score, LIS score and plasma syndecan-1 level were significantly higher than those in the survival group (P<0.05). The plasma level of SIRT-1 in patients with ALI/ARDS was negatively correlated with syndecan-1, APACHE Ⅱ score and LIS score, the plasma level of syndecan-1 was positively correlated with APACHEⅡ score and LIS score (P<0.05). Higher APACHEⅡ score, LIS score and plasma syndecan-1 were the risk factors of death in patients with ALI/ARDS, while higher plasma SIRT-1 was the protective factor of death in patients with ALI/ARDS (P<0.05). Values of plasma SIRT-1 and syndecan-1 in evaluating the prognosis of patients with ALI/ARDS were high, and the areas under the curve were 0.860 (95%CI: 0.791-0.930) and 0.870 (95%CI: 0.800-0.940) respectively. Conclusion The plasma SIRT-1 level is abnormally low and syndecan-1 level is abnormally high in patients with ALI/ARDS. The expression levels of SIRT-1 and syndecan-1 are closely related to the prognosis of patients. 数据和表 | 参考文献 | 相关文章 | 计量指标

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母血及羊水中TIM-3、GATA-3水平对胎膜早破合并宫内感染的诊断价值 王思思, 谢双双, 孟玥秀, 张翔云, 刘云春 2023, 51 (3):  315-319.  doi: 10.11958/20221201 摘要 ( 477 )   HTML ( 7 )   PDF(773KB) ( 1426 )   目的 分析母血及羊水中T细胞免疫球蛋白黏蛋白分子-3（TIM-3）、GATA结合蛋白3（GATA-3）水平对胎膜早破（PROM）合并宫内感染的诊断价值。方法 选择PROM患者89例作为研究对象，发生宫内感染者42例作为感染组，未发生宫内感染者47例作为未感染组，并选择同期正常足月孕妇45例作为对照组。根据宫内感染严重程度将PROM合并宫内感染患者分成轻度组16例、中度组17例、重度组9例。检测血、羊水中TIM-3、GATA-3 mRNA及蛋白水平，比较对照组、未感染组、感染组和不同感染程度孕妇血、羊水中TIM-3、GATA-3 mRNA及蛋白水平，受试者工作特征曲线分析血清及羊水中TIM-3、GATA-3蛋白单独及联合诊断PROM孕妇发生宫内感染的效能。结果 对照组、未感染组、感染组血及羊水中TIM-3 mRNA、蛋白水平依次升高（P<0.05），GATA-3 mRNA、蛋白水平依次降低（P<0.05）；轻度组、中度组、重度组血及羊水中TIM-3 mRNA、蛋白水平依次升高（P<0.05），GATA-3 mRNA、蛋白水平依次降低（P<0.05）；血清TIM-3、GATA-3蛋白单独及联合，羊水TIM-3、GATA-3蛋白单独及联合诊断PROM孕妇发生宫内感染的曲线下面积分别为0.854、0.813、0.937，0.834、0.850、0.922。结论 PROM合并宫内感染患者母血及羊水中TIM-3呈高表达，GATA-3呈低表达，两者均对PROM孕妇发生宫内感染有一定的诊断价值，联合诊断效能更高。 数据和表 | 参考文献 | 相关文章 | 计量指标

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慢性阻塞性肺疾病患者HRCT定量指标与肺功能的相关性研究 张凤, 方著, 袁艺, 周芸慧, 张国晋, 蒲红, 刘欢 2023, 51 (3):  320-324.  doi: 10.11958/20221162 摘要 ( 463 )   HTML ( 10 )   PDF(867KB) ( 1051 )   Objective To analyze quantitative indexes of emphysema and airway remodeling in patients with chronic obstructive pulmonary disease (COPD) by using high resolution CT (HRCT), and to explore the correlation between the above indexes and pulmonary function. Methods A total of 109 COPD patients (the COPD group) and 33 normal controls (the control group) were collected. According to the COPD Global Initiative guidelines, COPD patients were divided into the GOLD class Ⅰ group (n=29), the GOLD classⅡ group (n=38) and the GOLD class Ⅲ+Ⅳgroup (n=42). All patients underwent HRCT examination and pulmonary function examination. The post-processing software was used to automatically measure HRCT quantitative emphysema indexes of each lung lobe, including emphysema index (EI), mean lung density (MLD) of each lobe and airway quantitative indexes [bronchial wall area (WA), bronchial lumen area (LA) and the percentage of the wall area in the total bronchial section area (WA%)]. The above parameters were compared between the normal group and the different severity COPD subgroups. Spearman method was used to analyze the correlation between the above CT quantitative indexes and pulmonary function. Results Compared with the normal group, EI in upper lobe of both lungs was increased in the GOLD class Ⅰ group, EI in all lobes was increased in the GOLD class Ⅱ group, MLD values in upper lobe of both lungs and lower lobe of left lung were decreased, EI in all lobes was increased in the GOLD class Ⅲ+Ⅳ group, and MLD was decreased (P<0.05). Compared with the normal group, the WA of grade 5 bronchus and WA% of grade 4-6 bronchus were increased in the GOLD class Ⅰ group, and LA of grade 5 and 6 bronchus decreased. Compared with the GOLD class Ⅰ group, only WA and WA% of grade 6 bronchi were increased in the GOLD class Ⅱ group, and LA was decreased. Compared with the GOLD class Ⅱ group, WA% of grade 5 bronchial was decreased in the GOLD class Ⅲ+Ⅳ group (P<0.05). The measured and predicted percentages of forced expiratory volume in the first second (FEV1%) were negatively correlated with the EI of each lung lobe, and the MLD was positively correlated with FEV1% (P<0.01). The WA% of 5-6 bronchi was negatively correlated with FEV1% (P<0.05). Conclusion HRCT emphysema quantitative indexes and airway quantitative indexes have certain correlation with pulmonary function. It varies with different COPD grades, which can provide reference value for COPD evaluation. 数据和表 | 参考文献 | 相关文章 | 计量指标

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颅脑术后中枢神经系统感染的预测模型构建及初步应用 俞岚, 周林玲, 蒋伟 2023, 51 (3):  325-328.  doi: 10.11958/20221010 摘要 ( 473 )   HTML ( 6 )   PDF(722KB) ( 1065 )   Objective To establish an effective prediction model to evaluate the risk of central nervous system infection (CNSI) after craniotomy and to verify its feasibility. Methods A total of 1 020 patients with craniocerebral surgery in our hospital were selected. The indexes of postoperative infection were compared between the infection group (n=61) and the non infection group (n=959). Multivariate Logistic regression was used to establish risk prediction model and area test model under ROC curve to predict the effect. The effectiveness of the prediction model was preliminarily verified by 500 patients with craniocerebral operation. Results CNSI occurred in 61 cases (5.98%) of 1 020 patients undergoing craniocerebral surgery. Multivariate Logistic regression analysis showed that six risk factors including postoperative hospital stay, number of external ventricular drainage (EVD) use ≥1, EVD indwelling duration, operation duration, indwelling permanent implant and graft operation were included in the prediction model. The formula of the prediction model was as follows: postoperative CNSI=-3.025+1.354× postoperative hospital stay +1.225× number of EVD use +1.625×EVD indwelling time +1.427× operation time +1.221× implantation of permanent implants +1.218× consecutive surgery. The AUC under the ROC curve was 0.849 (95%CI: 0.761-0.915), the sensitivity was 81.56% and the specificity was 65.78%. In the preliminary validation cohort, 34 patients developed postoperative CNSI (6.8%), and the model predicted postoperative CNSI in 30 patients (6.0%), with a sensitivity of 91.48% and a specificity of 91.53%. Conclusion This model is suitable for the perioperative evaluation of patients with craniocerebral surgery, and can identify the high-risk population of postoperative CNSI in time. 数据和表 | 参考文献 | 相关文章 | 计量指标

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横纹肌溶解症常见并发症及治疗进展 靳衡, 刘起辉, 孙可可, 宋洁, 吕琪, 张艳 2023, 51 (3):  329-332.  doi: 10.11958/20221284 摘要 ( 752 )   HTML ( 15 )   PDF(705KB) ( 1487 )   横纹肌溶解症是一种由多种因素引起肌细胞破裂，细胞内容物释放入血而造成组织和器官损伤的综合征。最常见并发症为急性肾损伤，其他并发症有肝损伤、筋膜室综合征、电解质平衡紊乱以及弥散性血管内凝血等。目前常用治疗方法是液体复苏和碱化尿液，出现急性肾衰竭、高钾血症和代谢性酸中毒时，可采取血液透析治疗。新的治疗方面包括生物制剂和干细胞治疗，但多处于研究阶段，最终的临床疗效有待观察。因此明确横纹肌溶解症并发症机制及制定有效治疗方案可能是未来亟待解决的问题。就横纹肌溶解症相关常见并发症及治疗进展进行综述。 参考文献 | 相关文章 | 计量指标

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脓毒症相关肠道功能障碍发病机制的研究进展 胡英山, 王晶晶, 高红梅 2023, 51 (3):  333-336.  doi: 10.11958/20221492 摘要 ( 589 )   HTML ( 6 )   PDF(703KB) ( 1078 )   脓毒症相关肠道功能障碍是脓毒症患者较常见，但易被忽视的并发症。其发病机制尚未完全明确，目前可能的机制包括：肠道微生态失衡、异常的肠道细胞死亡、线粒体功能障碍等。由于此并发症的发病机制不清，且现有的治疗对患者预后的改善程度有限。该文就对脓毒症相关肠道功能障碍发病机制的研究现状进行综述，以期为脓毒症相关肠道功能障碍寻找新的临床治疗方案。 参考文献 | 相关文章 | 计量指标