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胰岛素抵抗对血小板内磷脂酰肌醇3-激酶活性影响的研究

皮淑芳1,邸阜生2,王怀祯3,王禹3,李鑫3   

  1. 1. 天津市第三中心医院 心脏中心
    2. 天津市第三中心医院内分泌科
    3. 天津市第三中心医院
  • 收稿日期:2010-02-08 修回日期:2010-03-10 出版日期:2010-07-15 发布日期:2010-07-15
  • 通讯作者: 皮淑芳

Influence of Insulin Resistance on Activity of PI-3-K in Platelet

  • Received:2010-02-08 Revised:2010-03-10 Published:2010-07-15 Online:2010-07-15

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摘要： 摘要 目的：探讨胰岛素抵抗对血小板内磷脂酰肌醇3-激酶（PI3-K）活性的影响。方法：比格犬24只，随机分为对照组及试验组，对照组动物给予普通喂养，试验组动物给予高脂喂养建立胰岛素抵抗动物模型，并以正常血糖-高胰岛素钳夹试验确认。6个月后确认胰岛素抵抗模型建立成功后检测两组动物血小板内磷脂酰肌醇3-激酶（PI3-K）活性及血小板粘附率、聚集率。结果：实验组动物血小板内PI3-K活性高于对照组（P＜0.05），血小板黏附率、聚集率增加（P＜0.001）。结论：胰岛素抵抗增加血小板内PI-3-K活性，是胰岛素抵抗导致血小板活性增强的关键环节。

关键词: 胰岛素抵抗, 血小板内磷脂酰肌醇3-激酶

Abstract: Abstract Objective: To study the influence of insulin resistance on activity of PI3-K in platelet. Methods: 24 beagle dogs were randomly divided into control group and test group .The animal's diet was normal in control group and high-fat in test group to establish the animal models of insulin resistance. Normal blood sugar–high insulin clamp test was performed after six months to confirm the animal models of insulin resistance. The PI3-K activity, aggregation rate and adhension rate of platelet were measured. Results: PI3-K activity of test group is higher than normal control group (P<0.05) and platelet adhesion rate, aggregation rate were increased in test group(P<0.001). Conclusion: Insulin resistance increased platelet PI3-K activity, which was the key to enhanced platelet activity.

Key words: insulin resistance, the activity of PI3-K in platelet