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Bax、Livin和Survivin在非小细胞肺癌组织中的表达

韩宝林   

  1. 天津市宝坻区人民医院
  • 收稿日期:2011-07-06 修回日期:2011-11-04 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 韩宝林

The Expression of Bax、Livin and Survivin in Non-Small Cell Lung Cancer

  • Received:2011-07-06 Revised:2011-11-04 Published:2012-05-15 Online:2012-05-15

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摘要： 目的：研究Bax、Livin和Survivin蛋白在非小细胞肺癌（NSCLC）组织中的表达情况及其与NSCLC病理因素的关系，探讨Bax、Livin和Survivin蛋白表达的相关性。方法：采用免疫组化非生物素二步法（PV）检测肺癌组织及正常肺组织中Bax、Livin和Survivin蛋白的表达情况，根据肿瘤组织细胞的染色率和染色强度进行判定。结果：正常肺组织及NSCLC组织中的阳性表达率Bax分别为80.00%和42.50%，Livin分别为10.00%和65.00%，Survivin分别为0和50.00%；有和无淋巴结转移患者的表达率Bax分别为23.81%、63.16%，Livin分别为85.71%、42.11%；Ⅰ、Ⅱ期和Ⅲ、Ⅳ期患者的表达率Bax分别为55.56%、15.38%，Livin分别为51.85%、92.31%；中高分化与低分化患者的Survivin表达率分别为39.29%、75.00%，以上差异均有统计学意义（P＜0.05）；NSCLC组织中Bax和Livin的表达呈负相关性（r＝－0.4294，P＜0.05）；Bax和Survivin，Livin和Survivin的表达均无相关性（P＞0.05）。结论：Bax在NSCLC中表达下调，Livin和Survivin在NSCLC中表达上调；Bax与Livin在NSCLC组织中的表达呈负相关性， Survivi与Bax、Survivi与Livin的表达均无明显相关性；检测Bax、Livin和Survivin的表达对NSCLC的诊断和预后评估有一定价值。

关键词: 非小细胞肺癌, Bax, Livin, Survivin

Abstract: Objective: To investigate the expression of Bax, Livin and Survivin and relationship between their expression and clinicopathological factors in non-small cell lung cancer, in order to provide further theoretical basis for clinical diagnosis and treatment of lungcancer. Methods：The expression of Bax, Livin and Survivin protein was detected by immunohistochemistry two-step techniques (PV). Results：The rates of positive expression of Bax、Livin and Survivin were respectively 42.50%、80.00%，65.00%、10.00% and 50.00%、0% in the non-small cell lung cancer tissues and in the normal lung tissues. The rates of positive expression of Bax and Livin were 23.81%、63.16% and 85.71%、42.11% in patients with lymph node metastasis and in patients with no lymph node metastasis.The rates of positive expression of Bax and Livin were 55.56%、15.38% and 51.85%、92.31% inⅠ, Ⅱstage patients and Ⅲ, Ⅳstage patients.The positive expression rate of Survivin in patients with well- middle-differentiated (39.29%) was significantly lower than that in patients with poorly differentiated(75.00%).The differences were all statistically significant (P <0.05).There was negative correlation between the expression of Bax and Livin in the non-small cell lung cancer（r＝－0.4294，P＜0.05）.There was no correlation between the expression of Bax and Survivin,and with no correlation between the expression of Livin and Survivin in the non-small cell lung cancer(P> 0.05). Conclusion: The increase of the expression of Bax in non-small lung cancer inhibited the invasiveness of tumors cell and tumors metastasi -s.Livin could be correlated with carcinogenesis of non-small cell lung cancer. The high-expression of Livin may enhanced the invasiveness of tumors cell and promoted tumors metastasis.Survivin could be correlated with tumors histologic -al differentiation degree and carcinogenesis of non-small cell lung cancer.There was negative correlation between the expression of Bax and Livin in the non-small cell lung cancer.It indicated that there could be the same pathway in the proceeding of non-small cell lung cancer. There was no correlation between the expression of Bax and Survivin, Livin and Survivin in the non-small cell lung cancer.It suggested that there could be a different pathway in the proceeding of non-small cell lung cancer.

Key words: non-small cell lung cancer, Bax, Livin, Survivin