天津医药

• 论著 •    下一篇

CRISP2基因在不同类型精子发生障碍中的表达及其意义

江莉,李慕军,覃莉   

  1. 广西医科大学第一附属医院
  • 收稿日期:2012-12-05 修回日期:2013-04-19 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 江莉

The Expression and Significance of CRISP2Gene in Different Types of Spermatogenic Failure

JIANG Li ,LI Mu jun,QIN Li   

  1. Reproductive Center, the First Affiliated Hospital of Guangxi Medical University
  • Received:2012-12-05 Revised:2013-04-19 Published:2013-11-15 Online:2013-11-15
  • Contact: JIANG Li

PDF (PC)

2257

摘要：

【摘要】 目的  探讨不同类型精子发生障碍男性精液中CRISP2mRNA的表达水平及其意义。方法  150例男性不孕患者分为正常组、弱精子组、少精子组各50例，提取精液细胞总RNA，通过实时荧光定量聚合酶链式反应（qRT-PCR）测定3组精子中CRISP2基因的mRNA转录水平并进行比较。 结果  CRISP2在正常组中的相对表达量（10.281±2.173）显著高于弱精组（2.092±0.969），差异有统计学意义（P<0.05），正常组与少精子组（9.420±2.794）差异无统计学意义（P>0.05），而弱精子组的CRISP2相对表达量也显著低于少精组（P<0.05）。  结论  CRISP2在不同精子发生障碍男性中的表达显著不同，CRISP2表达的减少可能导致精子活力下降。

关键词: 精子发生, RNA, RNA, 信使, 基因表达, CRISP2

Abstract:

[Abstract]   Objective  To study sperm mRNA transcript expression of CRISP2gene in the semen of patients with
different types of spermatogenetic failure. Methods  A total of150male infertility patients were divided into normal group,asthenozoospermia group and oligozoospermia group（n=50for each group）. Total RNA was extracted from sperm cells. Re-al-time quantitative reverse transcription polymerase chain reaction (qRT- PCR) method was used to detect CRISP2gene mRNA levels in three groups.   Results  There was a significantly higher expression of CRISP2in normal group (10.281±2.173) than that of asthenozoospermia group (2.092±0.969, P<0.05). There was no significant difference in the expression of CRISP2between normal group and oligozoospermia group (9.420±2.794, P>0.05). The relative expression of CRISP2was significantly lower in asthenozoospermia group than that of oligozoospermia group (P<0.05).   Conclusion  The CRISP2 expression was significantly different in patients with different types of spermatogenic failure. The reduced expression of CRISP2may lead to the decreased sperm motility.

Key words: Spermatogenesis, RNA, RNA, messenger, gene expression, cysteine-rich secretory protein2